English
新闻公告
More
化学进展 2010, Vol. 22 Issue (12): 2408-2419 前一篇   后一篇

• 综述与评论 •

双光子荧光探针

黄池宝1,2, 易道生1, 冯承浩1, 任安祥1, 孙世国2   

  1. 1. 韶关大学英东生物工程学院农业工程系 韶关 512005;
    2. 大连理工大学化工学院精细化工国家 重点实验室 大连 116012
  • 出版日期:2010-12-24 发布日期:2010-11-04
  • 作者简介:e-mail:renanxiang@163.com
  • 基金资助:

    国家自然科学基金项目(No.20872012)资助

Two-Photon Fluorescence Sensors

Huang Chibao1,2, Yi Daosheng1, Feng Chenghao1, Ren Anxiang1, Sun Shiguo2   

  1. 1. Department of Agricultural Engineering, Yingdong Bioengineering College, Shaoguan University, Shaoguan 512005, China;
    2. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China
  • Online:2010-12-24 Published:2010-11-04

双光子吸收是指在强光激发下,介质分子同时吸收两个光子,从基态跃迁到两倍光子能量的激发态的过程。荧光显微成像是研究活体生物的重要工具,而最通常的细胞成像方法则是使用单光子激发荧光团的单光子显微成像。近红外光源激发的双光子荧光探针克服了单光子荧光探针的光漂白与光致毒而更适于生物检测与成像,为生命科学研究提供了更为锐利的工具。双光子荧光探针的作用机理包括分子内电荷迁移(ICT)、荧光共振能量迁移(FRET)、光诱导电子迁移(PET)与基团转换(GC) 4种方式。该文综述了双光子阳离子探针(Mg2+, Ca2+, Pb2+, Hg2+, Ag+, Fe3+, Zn2+, Na+, Cr3+)、双光子阴离子探针(F-)、pH探针、双光子葡萄糖示踪器、双光子脂筏探针、双光子巯基探针、双光子半胱氨酸探针和双光子生物标记探针,以及双光子荧光探针在生物成像方面的应用,展望了双光子荧光探针的发展趋势与应用前景。

Two-photon absorption is a process in which a molecule in medium simultaneously absorbs two photons, and performs transition from ground state to excited state with the energy of two photons. Optical imaging with fluorescence microscopy is a vital tool in the study of living cells and tissues, whereas the most common method for cell imaging, one-photon microscopy (OPM), uses a single photon of higher energy to excite the fluorophore. By using NIR (near infrared) photons as excitation source, two-photon fluorescence sensors which can avoid photodamage and photobleaching induced by one-photon fluorescence sensors are quite suitable for bioassay and bioimaging, and become powerful tools for the study on life science. The recognition mechanisms of two-photon fluorescence probes are comprised of the intramolecular charge transfer (ICT), the fluorescence resonance energy transfer (RET), the photoinduced electron transfer (PET) and the group conversion (GC). Two-photon probes for cation ions (Mg2+, Ca2+, Pb2+, Hg2+, Ag+, Fe3+, Zn2+, Na+, Cr3+), two-photon anion ion probes (F-), two-photon pH probes, two-photon glucose tracers, two-photon lipid raft probes, two-photon sulfhydryl group probes, two-photon cysteine probes and two-photon biolabelling probes, and the applications of two-photon fluorescence probes in bioimaging are reviewed. In particular, the research progress, developing trends and prospects in the future are also discussed.

Contents
1 Introduction
2 Two-photon probes for cation ions
2.1 Two-photon probes for magnesium ions
2.2 Two-photon probes for calcium ions
2.3 Two-photon probes for lead ions
2.4 Two-photon probes for mercury ions
2.5 Two-photon probes for silver ions
2.6 Two-photon probes for ferric ions
2.7 Two-photon probes for zinc ions
2.8 Two-photon probes for sodium ions
2.9 Two-photon probes for chromium ions
3 Two-photon probes for anion ions
3.1 Two-photon probes for fluorine ions
4 Two-photon probes for pH
4.1 Phenolic hydroxyl group ligand
4.2 Arylamino group ligand
4.3 Pyridine acid ligand
5 Two-photon glucose tracer
6 Two-photon probes for lipid rafts
7 Two-photon probes for sulfhydryl group
8 Two-photon probes for cysteine
9 Two-photon probes for biolabelling
10 Conclusion and outlook

中图分类号: 

()


[1] Gppert-Mayer M. Ann. Phys., 1931, 9: 273—273

[2] Kaiser W, Garrett C G B. Phys. Rev. Lett., 1961, 123 (1): 229—231

[3] Denk W, Strickler J H, Webb W W. Science, 1990, 248 (4951): 73—76

[4] Albota M, Beljonne D, Brédas J L, Ehrlich J E, Fu J Y, Heikal A A, Hess S E, Kogej T, Levin M D, Marder S R, McCord-Maughon D, Perry J W, Rckel H, Rumi M, Subramaniam G, Webb W W, Wu X L, Xu C. Science, 1998, 281 (5383): 1653—1656

[5] Shen Y Z, Jakubczyk D, Xu F, Swiatkiewicz J, Prasad P N. Appl. Phys. Lett., 2000, 76 (1): 1—3

[6] Ventelon L, Charier S, Moreaux L, Mertz J, Blanchard-Desce M. Angew. Chem. Int. Ed., 2001, 40 (11): 2098—2101

[7] Helmchen F, Denk W. Curr. Opin. Neurol., 2002, 12 (5): 593—601

[8] Huang Z L, Li N, Lei H, Qiu Z R, Wang H Z, Zhong Z P, Zhou Z H. Chem. Commun., 2002, (20): 2400—2401

[9] Liu Z Q, Fang Q, Wang D, Xue G, Yu W T, Shao Z S, Jiang M H. Chem. Commun., 2002, (23): 2900—2901

[10] Pond S J K, Tsutsumi O, Rumi M, Kwon O, Zojer E, Bredas J L, Marder S R, Perry J W. J. Am. Chem. Soc., 2004, 126 (30): 9291—9306

[11] Kim H M, Jung C, Kim B R, Jung S Y, Hong J H, Ko Y G, Lee K J, Cho B R. Angew. Chem. Int. Ed., 2007, 46: 3460—3463

[12] Kim H M, Yang P R, Seo M S, Yi J S, Hong J H, Jeon S J, Ko Y G, Lee K J, Cho B R. J. Org. Chem., 2007, 72 (6): 2088—2096

[13] Kim H M, Jeong M Y, Ahn H C, Jeon S J, Cho B R. J. Org. Chem., 2004, 69 (17): 5749 —5751

[14] Kim H M, Kim B R, Hong J H, Park J S, Lee K J, Cho B R. Angew. Chem. Int. Ed., 2007, 46: 7445—7448

[15] Dong X, Yang Y, Sun J, Liu Z, Liu B F. Chem. Commun., 2009, 3883—3885

[16] Mohan P S, Lim C S, Tian Y S, Roh W Y, Leeb J H, Cho B R. Chem. Commun., 2009, 5365—5367

[17] Ahn H C, Yang S K, Kim H M, Li S, Jeon S J, Cho B R. Chem. Phys. Lett., 2005, 410 (4/6): 312—315

[18] Kim J S, Kim H J, Kim H M, Kim S H, Lee J W, Kim S K, Cho B R. J. Org. Chem., 2006 , 71 (21): 8016—8022

[19] 崔景强 (Cui J Q), 樊江莉 (Fan J L), 彭孝军 (Peng X J), 孙世国 (Sun S G), 陈贵财 (Chen G C), 郭柯馨 (Guo K X). 中国科学 B辑: 化学 (Science in China Series B:Chemistry), 2009, 39 (6): 541—547

[20] Huang C, Fan J, Peng X, Lin Z, Guo B, Ren A, Cui J, Sun S. J. Photochem. Photobiol. C, 2008, 199: 144—149

[21] Huang C, Peng X, Lin Z, Fan J, Ren A, Sun D. Sensors and Actuators B: Chemical, 2008, 133: 113—117

[22] 黄池宝 (Huang C B), 任安祥 (Ren A X), 黄志宏 (Huang Z H), 郑立军 (Zheng L J), 李海渤 (Li H B), 冯承浩 (Feng C H). 分析化学 (Chinese Journal of Analytical Chemistry), 2010, 38 (4): 464—468

[23] Taki M, Wolford J L, OHalloran T V. J. Am. Chem. Soc., 2004, 126: 712—713

[24] Bhaskar A, Ramakrishna G, Twieg R J, Goodson T. J. Phys. Chem. C, 2007, 111 (40): 14607—14611

[25] Sumalekshmy S, Henary M M, Siegel N, Lawson P V, Wu Y, Schmidt K, Brédas J L, Perry J W, Fahrni C J. J. Am. Chem. Soc., 2007, 129 (39): 11888—11889

[26] Kim H M, Seo M S, An M J, Hong J H, Tian Y S, Choi J H, Kwon O, Lee K J, Cho B R. Angew. Chem. Int. Ed., 2008, 47: 5167—5170

[27] Kim M K, Lim C S, Hong J T, Han J H, Jang H Y, Kim H M, Cho B R. Angew. Chem. Int. Ed., 2010, 49: 364—367

[28] Wan Y, Guo Q, Wang X, Xia A. Analytica Chimica Acta, 2010, 665: 215—220

[29] Liu Z Q, Shi M, Li F-Y, Fang Q, Chen Z H, Yi T, Huang C H. Org. Lett., 2005, 7: 5481—5484

[30] Cao D, Liu Z, Li G. Sensors and Actuators B, 2008, 133: 489—492

[31] Zhang M, Li M, Li F, Cheng Y, Zhang J, Yi T, Huang C. Dyes and Pigments, 2008, 77: 408—414

[32] Morales A R, Schafer-Hales K J, Yanez C O, Bondar M V, Przhonska O V, Marcus A I, Belfield K D. Chem. Phys. Chem., 2009, 10: 2073—2081

[33] Werts M H V, Gmouh S, Mongin O, Pons T, Blanchard-Desce M. J. Am. Chem. Soc., 2004, 126 (50): 16294—16295

[34] Kim H M, An M J, Hong J H, Jeong B H, Kwon O, Hyon J Y, Hong S C, Lee K J, Cho B R. Angew. Chem. Int. Ed., 2008, 47: 2231—2234

[35] Xiao H, Li H, Chen M, Wang L. Dyes and Pigments, 2009, 83: 334—338

[36] Tian Y S, Lee H Y, Lim C S, Park J, Kim H M, Shin Y N, Kim E S, Jeon H J, Park S B, Cho B R. Angew. Chem. Int. Ed., 2009, 48: 8027—8031

[37] Kim H M, Jeong B H, Hyon J Y, An M J, Seo M S, Hong J H, Lee K J, Kim C H, Joo T, Hong S C, Cho B R. J. Am. Chem. Soc., 2008, 130 (13): 4246—4247

[38] Lee J H, Lim C S, Tian Y S, Han J H, Cho B R. J. Am. Chem. Soc., 2010, 132 (2): 1216—1217

[39] Zhang M, Li M, Zhao Q, Li F, Zhang D, Zhang J, Yia T, Huang C. Tetrahedron Letters, 2007, 48: 2329—2333

[40] Zhang M, Yu M, Li F, Zhu M, Li M, Gao Y, Li L, Liu Z, Zhang J, Zhang D, Yi T, Huang C. J. Am. Chem. Soc., 2007, 129 (34): 10322—10323

[41] Zhang X, Ren X, Xu Q H, Loh K P, Chen Z K. Org. Lett., 2009, 11 (6): 1257—1260

[42] Morales A R, Yanez C O, Schafer-Hales K J, Marcus A I, Belfield K D. Bioconjugate Chem., 2009, 20 (10): 1992—2000

[1] 黄池宝*, 陈绍英. 双光子荧光探针[J]. 化学进展, 2017, 29(10): 1215-1227.
[2] 黄池宝,樊江莉,彭孝军,孙世国.

双光子荧光探针研究及其应用*

[J]. 化学进展, 2007, 19(11): 1806-1812.
阅读次数
全文


摘要

双光子荧光探针