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• 综述与评论 •

蛋白功能化磁性纳米颗粒的制备及应用

黄天天, 付雁, 张金利, 李韡*   

  1. 天津大学化工学院 天津 300072
  • 收稿日期:2012-01-01 修回日期:2012-03-01 出版日期:2012-08-24 发布日期:2012-08-06
  • 通讯作者: 李韡 E-mail:liwei@tju.edu.cn
  • 基金资助:

    国家自然科学基金项目(No.20836005, 21076141)资助

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导出 被引次数 ( 9 | 2 )

Preparation and Application of Protein-Functionalized Magnetic Nanoparticles

Huang Tiantian, Fu Yan, Zhang Jinli, Li Wei   

  1. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
  • Received:2012-01-01 Revised:2012-03-01 Online:2012-08-24 Published:2012-08-06
蛋白功能化磁性纳米颗粒作为一种新型功能复合材料,已成为众多领域的研究热点。蛋白质在颗粒表面的稳定性、负载量及构象显著影响复合粒子的应用性能,而磁性纳米颗粒表面物化性质对颗粒的稳定性、分散性及磁性能对蛋白质的高效负载有重要影响。本文综述了磁性纳米颗粒表面修饰、蛋白功能化的方法以及蛋白在界面处构象变化的表征方法,介绍了蛋白功能化磁性纳米颗粒在酶催化合成、免疫分析检测及生物传感器等领域的应用,并对其未来的发展趋势进行了展望。
关键词： 磁性纳米颗粒, 表面修饰, 蛋白功能化, 吸附, 共价
Protein-functionalized magnetic nanoparticles have received considerable attention in many research areas as new functional composite materials. The stability, loading capacity and the conformation retention of the protein affect the application of the functional particles. The physicochemical properties of the surface not only affect the stability, dispersion and magnetism but also the effective loading of protein. In this paper, the surface modification of magnetic nanoparticles and the protein-functionalized methods are reviewed. Major applications of protein-functionalized magnetic nanoparticles are introduced in the field of enzymatic synthesis, detection analysis of immunoreactions, and biosensors, as well as the future developing prospects. Contents 1 Introduction
2 Preparation of protein-functionalized magnetic nanoparticles
2.1 Adsorption-based immobilization
2.2 Covalent immobilization
3 Application of protein-functionalized magnetic nanoparticles
3.1 Enzymatic synthesis
3.2 Detection analysis
3.3 Biosensors
4 Characterization of proteins immobilized on surfaces
5 Conclusion and outlook
Key words: magnetic nanoparticles, surface modification, protein-functionalization, adsorption, covalent

中图分类号: 

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