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化学进展 2012, Vol. 24 Issue (05): 852-862 前一篇   后一篇

• 综述与评论 •

支撑磷脂双层膜的研究进展

张治磊, 王志宁, 高学理, 高从堦*   

  1. 中国海洋大学化学化工学院 海洋化学理论与工程技术教育部重点实验室 青岛 266100
  • 收稿日期:2011-09-01 修回日期:2011-12-01 出版日期:2012-05-24 发布日期:2012-04-10
  • 基金资助:
    国家自然科学基金项目(No.21106139,21006100)和山东省中青年科学家科研奖励基金(No.BS2011CL040)资助
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Progress in Supported Phospholipid Bilayers

Zhang Zhilei, Wang Zhining, Gao Xueli, Gao Congjie   

  1. Key Laboratory of Marine Chemistry Theory and Technology of Ministry of Education, College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China
  • Received:2011-09-01 Revised:2011-12-01 Online:2012-05-24 Published:2012-04-10
支撑磷脂双层膜(supported phospholipid bilayers, SPBs)是细胞膜研究中普及的模型,是固定生物活性物质的理想材料,不仅可以保持生物分子的活性,还能有效抑制其他生物分子的非特异性吸附,在跨膜蛋白、仿生膜、水处理、生物医学和生物传感器等研究领域具有广泛的应用前景。本文介绍了支撑磷脂双层膜的表征方法和制备方法,包括Langmuir Blodgett(LB)膜提拉法、囊泡融合法和LB膜提拉法与囊泡融合联合法;详细阐述了囊泡融合法制备SPBs的机理;综述了囊泡融合法制备SPBs的影响因素,包括囊泡浓度、缓冲溶液、温度、囊泡和基底表面电荷等因素;列举了支撑磷脂膜的应用,并展望了支撑磷脂双层膜的研究趋势。
关键词： 支撑磷脂双层膜, 形成机理, LB膜提拉法, 囊泡融合法
Supported phospholipid bilayers(SPBs) are popular as model systems for cell membranes and are ideal material for fixing biological active substances. In addition, SPBs can not only maintain the biological activity of molecules, but also inhibit other non-specific adsorption of biological molecules. SPBs are promising for future applications in the transmembrane protein, biomimetics, treating water, biomedicine and biosensors. The characterization and preparation methods of SPBs are reviewed, which include Langmuir-Blodgett technique, lipid vesicles fusion approach, and the combination of Langmuir-Blodgett technique and vesicles fusion approach. It is described in detail that the formation mechanisms of SPBs triggered by vesicles fusion approach. The factors of the formation of SPBs are also reviewed in this paper, including the concentration of vesicles, buffer solution, temperature, charge of vesicles and the solid surface. In the end, the applications of SPBs and the latest trends in the study of SPBs are predicted.

Contents
1 Introduction
2 Characterization methods of SPBs
3 Preparation methods of SPBs
3.1 Langmuir-Blodgett technique
3.2 Vesicle fusion
3.3 Combination of Langmuir-Blodgett monolayer transfer and vesicle fusion
4 Influence factor
4.1 Influencing of surface charge about vesicle and support
4.2 Influencing of buffer solution
4.3 Influencing of temperature
4.4 Influencing of vesicle concentration
4.5 Influencing of surface property
4.6 Influencing of osmotic pressure
5 Application
5.1 Biomembrane model
5.2 Sensors
5.3 Biomedicine
5.4 Treating water
6 Prospects

Key words: supported phospholipid bilayers(SPBs), formation mechanisms, Langmuir-Blodgett technique, vesicles fusion

中图分类号: 

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支撑磷脂双层膜的研究进展