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化学进展 2012, Vol. 24 Issue (01): 173-181 前一篇   后一篇

• 综述与评论 •

间充质干细胞扩增载体材料

赵爽1, 赵燕燕1, 孟恒星2, 李茜2, 尹玉姬1*   

  1. 1. 天津大学材料科学与工程学院 天津市材料复合与功能化重点实验室 天津 30007;
    2. 协和干细胞基因工程有限公司 天津 300384
  • 收稿日期:2011-05-01 修回日期:2011-06-01 出版日期:2012-01-24 发布日期:2011-11-22
  • 基金资助:

    国家自然科学基金项目(No.51073116,30670572)资助

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导出 被引次数 ( 12 | 1 )

Carrier Materials of Mesenchymal Stem Cells Expansion

Zhao Shuang1, Zhao Yanyan1, Meng Hengxing2, Li Qian2, Yin Yuji1*   

  1. 1. Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin 30007;
    2. Union Stem Cell & Gene Engineering CO. LTD., Tianjin 300384, China
  • Received:2011-05-01 Revised:2011-06-01 Online:2012-01-24 Published:2011-11-22

间充质干细胞(MSCs)具有高度自我更新能力、多分化潜能、体外易分离和培养的特性,是细胞治疗和组织工程重要的种子细胞来源,但如何大规模地获得具有可再生活性的MSCs一直是限制其临床应用的关键因素,近几年发展起来的贴壁动物细胞动态培养技术为MSCs的大规模体外扩增提供了一条重要的途径。本综述结合动物细胞扩增载体的发展现状,主要介绍了用于间充质干细胞三维动态培养的明胶载体、海藻酸盐载体、壳聚糖载体和其他多糖载体等常规载体及其表面修饰和改性方法,并进一步介绍了以非酶解途径回收扩 增细胞的新型干细胞载体的研究进展。随着新型载体材料的涌现以及人们对干细胞生长和扩增特点的了解,采用三维动态培养技术安全而有效地大规模体外扩增MSCs的必要性将得到进一步的确认。

关键词： 间充质干细胞, 扩增, 载体材料, 表面修饰, 三维动态培养

Mesenchymal stem cell (MSC) is an important cell source of cell therapy and tissue engineering because of its characteristics of self-renewal, multi-differentiation potential, easily isolated and cultured in vitro. Expansion of MSCs in vitro is a necessary step in clinical application of MSCs since it is impossible to get enough MSCs directly from donors. Namely, how to culture MSCs in large-scale is the key factor to limit its application. The methodology of 3-D dynamic culture of anchorage-dependent cells provides an important way for expansion of MSCs in vitro. This review intends to overview the current progress in the MSCs expansion field and discusses the main events that have occurred along the way. Gelatin, alginate, chitosan and some other polysaccharide carrier materials used for 3-D culture of mammalian cells and MSCs are summarized and discussed. The surface modification methodologies of the microcarriers are also presented. Furthermore, some new carrier materials used for stem cells expansion are introduced. The technical advances together with the ever increasing knowledge and experience in the field of carrier materials preparation and MSCs proliferation/expansion characteristics will lead to the realization of the full potential of 3-D dynamic MSCs culture in the future.

Contents
1 Intruduction
2 Conventional carriers of animal cell culture
2.1 Gelatin microcarriers
2.2 Alginate microcarriers
2.3 Chitosan microcarriers
2.4 Other polysaccharide carriers
3 Novel stem cells carriers
4 Prospect

Key words: mesenchymal stem cells (MSCs), expansion, carrier materials, surface modification, 3-D dynamic culture

中图分类号: 

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摘要

间充质干细胞扩增载体材料