English
往期目录
新闻公告
More
化学进展 2012, Vol. 24 Issue (01): 122-130 前一篇   后一篇

• 综述与评论 •

硅胶基质高效液相色谱填料研究进展

赵贝贝1, 张艳1, 唐涛1,2, 王风云1*, 张维冰2,3, 李彤2   

  1. 1. 南京理工大学工业化学研究所 南京210094;
    2. 大连依利特分析仪器有限公司 大连11602;
    3. 华东理工大学上海市功能性材料化学重点实验室 上海 200237
  • 收稿日期:2011-05-01 修回日期:2011-06-01 出版日期:2012-01-24 发布日期:2011-11-22
  • 基金资助:

    国家重点基础研究发展计划(973)项目(No.2007CB914100,2011CB910404),国家自然科学基金(No.21075039/B050106),江苏省科技支撑计划社会发展项目(No.BE2010731)和大连市科学技术基金计划项目(No.2009J22DW015)资助

下载PDF ( 1796 ) 引用
导出 被引次数 ( 25 | 13 )

Silica Based Stationary Phases for High Performance Liquid Chromatography

Zhao Beibei1, Zhang Yan1, Tang Tao1,2, Wang Fengyun1*, Zhang Weibing2,3, Li Tong2   

  1. 1. Chemical Engineering Institute, Nanjing University of Science and Technology, Nanjing 210094, China;
    2. Dalian Elite Analytical Instruments Co., Ltd., Dalian 11602;
    3. Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, China
  • Received:2011-05-01 Revised:2011-06-01 Online:2012-01-24 Published:2011-11-22

高效液相色谱(HPLC)不仅是一种有效的分析分离手段,也是一种重要的高效制备分离技术。色谱柱是HPLC系统的核心,不同性能的填料是HPLC广泛应用的基础。硅胶是开发最早、研究最为深入、应用最为广泛的HPLC固定相基质,其制备方法主要有喷雾干燥法、溶胶-凝胶法、聚合诱导胶体凝聚法及模板法等。近年来,亚2μm小粒径硅胶、核-壳型硅胶、双孔径硅胶、介孔性硅胶、有机杂化硅胶等新型硅胶应用于HPLC并取得了色谱分离技术的飞速发展,例如基于亚2μm填料的超高压液相色谱技术、基于核-壳型填料的快速分离技术、基于杂化硅胶填料的高温液相色谱技术等。硅胶经表面化学键合、聚合物包覆等有机改性可制得先进的大分子限进填料、温敏性填料、手性填料等,大大扩展了HPLC的应用范围。本文对液相色谱用硅胶的制备方法、改性与修饰方法以及硅胶基质固定相的评价方法加以系统综述,概述了新型硅胶在HPLC中的应用进展,并对硅胶基质填料的发展方向与应用前景进行了展望。

关键词： 高效液相色谱, 硅胶, 填料

High performance liquid chromatography is not only a useful analytical technique, but also an effective preparation method. The availability of a variety of stationary phases for column has been a key factor in the development of HPLC as a major scientific tool. With the most desirable compromise of properties that provide for effective and reproducible separations, silica has been the most widely used HPLC packing material. The silica microspheres are synthesized by various methods, including spray drying, sol-gel, polymerization induced colloid aggregation and templating methods. In recent years, atypical types of silica are prepared and applied in HPLC, such as sub-2μm silica particles, superficially porous silica particles, bimodal silica particles, mesoporous silica particles, organic/silica hybrid particles, etc. As a result, unique separation properties that enlarge the capabilities of HPLC methods have been achieved, such as ultrahigh-pressure liquid chromatography based on sub-2μm silica particles, fast liquid chromatography based on superficially porous silica particles, high temperature liquid chromatography based on organic/silica hybrid particles. Moreover, novel stationary phase can be obtained by chemical bonding or polymer modification of silica surface, such as chiral stationary phase, temperature-responsive stationary phase and restricted access materials. In this paper, the preparation methods and modification modes of silica particles are introduced, as well as the characterization methods of HPLC stationary phase. The applications of silica packing material in HPLC and its developing trends are also outlined.

Contents
1 Introduction
2 Preparation methods of silica
2.1 Spray drying method
2.2 Polymerization induced colloid aggregation method
2.3 One-step catalytic sol-gel method
2.4 Two-step catalytic sol-gel method
2.5 Templating method
2.6 Preparation methods of atypical silica
2.7 Preparation methods of organic/silica hybrid particles
3 Modification methods and characterization methods of silica
3.1 Modification of silica
3.2 Characterization of silica based packing materials
4 Application of silica based packing materials
4.1 Application of atypical silica
4.2 Application of organic/silica hybrid particles
5 Conclusions and outlook

Key words: high performance liquid chromatography (HPLC), silica, packing material

中图分类号: 

()

[1] Unger K K, Skudas R, Schulte M M. J. Chromatogr. A, 2008, 1184: 393-415
[2] Nawrocki J, Dunlap C, Mccormick A, Carr P W. J. Chromatogr. A, 2004, 1028: 1-30
[3] Paek C, McCormick A V, Carr P W. J. Chromatogr. A, 2010, 1217: 6475-6483
[4] Huai Q Y, Wang X L, Zuo Y M. Chromatographia, 2002, 55: 637-645
[5] Kirkland J J, DeStefano J J. J. Chromatogr. A, 2006, 1126: 50-57
[6] Ai F, Li L, Ng S C, Tan T T Y. J. Chromatogr. A, 2010, 1217: 7502-7506
[7] Unger K K, Kumar D, Grun M, Buchel G, Ludtke S, Adam T, Schumacher K, Renker S. J. Chromatogr. A, 2000, 892: 47-55
[8] Uzun L, Yavuz H, Say R. Ind. Eng. Chem. Res. , 2004, 43: 6507-6513
[9] Haginaka J. J. Chromatogr. B, 2008, 866: 3-13
[10] West C, Elfakir C, Lafosse M. J. Chromatogr. A, 2010, 1217: 3201-3216
[11] Davies N H, Euerby M R, McCaller D V. J. Chromatogr. A, 2008, 1178: 71-78
[12] Pelletier S, Lucy C A. J. Chromatogr. A, 2006, 1125: 189-194
[13] Pesek J J, Matyska M T. J. Sep. Sci. , 2009, 32: 3999-4011
[14] 佟巍(Tong W), 张养军(Zhang Y J), 秦伟捷(Qin W J), 钱小红(Qian X H). 色谱(Chinese of Chromatography), 2010, 28(10): 915-922
[15] Qiu H D, Liang X J, Sun M, Jiang S X. Anal. Bioanal. Chem., 2011, 399: 3307-3322
[16] Kang Y J, Shan W, Wu J Y. Chem. Mater. , 2006, 18(7): 1861-1866
[17] Zhao L, Yu J G, Guo R, Cheng B. Key Eng. Mater., 2005, 280/283: 1153-1156
[18] 张庆合(Zhang Q H). 大连化学物理研究所博士后出站报告(Post Doctoral Dissertation of Dalian Institute of Chemical Physics Chinese of Academy of Sciences), 2002
[19] Chang S M, Lee M, Kim W S. J. Colloid Interface Sci. , 2005, 286: 536-542
[20] Rahman I A, Vejayakumaran P, Sipaut C S, Ismail J, Baker M A, Adnan R, Chee C K. Ceram. Int. , 2006, 32: 691-699
[21] Wang X D, Shen Z X, Sang T, Cheng X B, Li M F, Chen L Y. J. Colloid Interface Sci. , 2010, 341: 23-29
[22] Zhao B B, Tian C H, Zhang Y, Tang T, Wang F Y. Particuology, 2011, 9: 314-317
[23] Bhagat S D, Rao A V. Appl. Surf. Sci., 2006, 252: 4289-4297
[24] Rao A V, Bhagat S D. Solid State Sci., 2004, 6: 945-952
[25] Choi D G, Yang S M. J. Colloid Interf. Sci., 2003, 261: 127-132
[26] Hohenesche C F, Ehwald V, Unger K K. J. Chromatogr. A, 2004, 1025: 177-187
[27] 赵贝贝(Zhao B B), 许婵婵(Xu C C), 唐涛(Tang T), 李彤(Li T), 张维冰(Zhang W B), 王风云(Wang F Y). 无机材料学报(Chinese of Journal of Inorganic Materials), 2011, 26: 1090-1094
[28] 杨新立(Yang X L). 中国科学院大连化学物理研究所博士论文(Doctoral Disseratation of Dalian Institute of Chemical Physics Chinese of Academy of Sciences), 2001
[29] 赵贝贝(Zhao B B), 许婵婵(Xu C C), 唐涛(Tang T), 李彤(Li T), 张维冰(Zhang W B), 王风云(Wang F Y). 分析化学(Chinese of Analytical Chemistry), 2011, 39: 1243-1246
[30] Wu Y J, Ren X Q, Wang J. Mater. Chem. Phys. , 2009, 113: 773-779
[31] Tian R J, Sun J M, Zhang H, Ye M L, Xie C H, Dong J, Hu J W, Ma D, Bao X H, Zou H F. Electrophoresis, 2006, 27: 742-748
[32] Poyraz A S, Albayrak C, Dag O. Microporous Mesoporous Mater., 2008, 115: 548-555
[33] Li Y Y, Cheng S Y, Dai P C, Liang X M, Ke Y X. Chem. Commun., 2009, 1085-1087
[34] Hao W Q, Di B, Liu H, Hang T J, Yan F, Su M X. Chromatographia, 2011, 73: 623-629
[35] Wan H H, Liu L, Li C M, Xue X Y, Liang X M. J. Colloid Interf. Sci., 2009, 337: 420-426
[36] Liu X B, Li L S, Du Y, Guo Z, Ong T T, Chen Y, Ng S C, Yang Y H. J. Chromatogr. A, 2009, 1216: 7767-7773
[37] Ai F, Li L S, Ng S C, Tan T T Y. J. Chromatogr. A, 2010, 1217: 7502-7506
[38] Yang L M, Wang Y J, Sun Y W, Luo G S, Dai Y Y. J. Colloid Interf. Sci., 2006, 299: 823-830
[39] Yang L M, Wang Y J, Luo G S, Dai Y Y. Particuology, 2008, 6: 143-148
[40] Kirkland J J, Truszkowski F A, Ricker R D. J. Chromatogr. A, 2002, 965: 25-34
[41] Kirkland J J, Truszkowski F A, Dilks J C H, Engel G S. J. Chromatogr. A, 2000, 890: 3-13
[42] Faria A M, Magalhaes D R, Collins K E, Collins C H. Anal. Chim. Acta, 2005, 550: 137-143
[43] Silva C R, Airoldi C, Collins K E, Collins C H. J. Chromatogr. A, 2008, 1191: 90-98
[44] Collins C H, Silva C R, Faria A M, Collins K E, Jardim I C S F. J. Braz. Chem. Soc., 2009, 20: 604-612
[45] Liang X, Liu S, Liu H, Liu X, Jiang S. J. Sep. Sci., 2010, 33: 3304-3312
[46] Nakanishi K, Minakuchi H, Soga N, Tanaka N. J. Sol-Gel Sci. Technol., 1998, 13: 163-169
[47] Wei J X, Shi Z G, Chen F, Feng Y Q, Guo Q Z. J. Chromatogr. A, 2009, 1216: 7388-7393
[48] Novakova L, Matysova L, Solich P. Talanta, 2006, 68: 908-918
[49] Jiang Z P, Fisk R P, Gara J O, Walter T H, Wyndham K D. US 2007/0243383, 2007
[50] Guo R R, Ding M Y. Colloids Surf. A, 2007, 292: 153-158
[51] Li Y S, Li B, Han N Y, Xu B J. J. Chromatogr. A, 2003, 1021: 183-189
[52] Kirkland J J. J. Chromatogr. A, 2004, 1060: 9-21
[53] Luo H, Carr P W. Anal. Bioanal. Chem., 2008, 391: 919-923
[54] McCalley D V. J. Chromatogr. A, 2010, 1217: 858-880
[55] Chice J, Meca S, Companyo R, Prat M D, Granados M. J. Chromatogr. A, 2008, 1181: 1-8
[56] He Z Y, Lv C G, Fan X X, Zhou Z Q. Anal. Chim. Acta, 2011, 689(1): 143-148
[57] Tello A M, Lebron-Aguilar R, Quintanilla-Lopez J E, Santiuste J M. J. Chromatogr. A, 2009, 1216: 1630-1639
[58] Tamayo F G, Titirici M M, Martin-Esteban A, Sellergren B. Anal. Chim. Acta, 2005, 542: 38-46
[59] Burdukova E, Li H, Ishida N, Shea J P O, Franks G V. J. Colloid Interf. Sci. , 2010, 342: 586-592
[60] Kanazawa H, Ayano E, Sakamoto C, Yoda R, Kikuchi A, Okano T. J. Chromatogr. A, 2006, 1106: 152-158
[61] Mallik A K, Rahman M M, Czaun M, Takafuji M, Ihara H. J. Chromatogr. A, 2008, 1187: 119-127
[62] Sadilek P, Satinsky D, Solich P. Trends Anal. Chem., 2007, 26: 375-384
[63] Kurpczynska K, Buszewski B, Jandera P. Anal. Chem., 2004, 76: 227-234
[64] Faria A M, Magalhaes D R, Collins K E, Collins C H. Anal. Chim. Acta, 2005, 550: 137-143
[65] Xiong C Q, Zhou X Y, Chen R, Zhang Y M, Peng W P, Nie Z X, Chang H C, Liu H W, Chen Y. Anal. Chem., 2011, 83: 5400-5406
[66] Buszewski B, Bocian S, Felinger A. J. Chromatogr. A, 2008, 1191: 72-77
[67] Dehouck P, Visky D, Heyden Y V, Adams E, Kovacs Z, Noszal B, Massart D L, Hoogmartens J. J. Chromatogr. A, 2004, 1025: 189-200
[68] Standard Reference Materia®869a. National Institute of Standard & Technology, 2002
[69] Standard Reference Materia®870a. National Institute of Standard & Technology, 2002
[70] Visky D, Heyden Y V, Ivanyi T, Baten P, Beer J D, Kovacs Z, Noszal B, Dehouck P, Roets E, Massart D L, Hoogmartens J. J. Chromatogr. A, 2003, 1012: 11-29
[71] Ye C, Terfloth G, Li Y, Kord A. J. Pharm. Biomed. Anal., 2009, 50: 429-431
[72] Claessens H A, Straten M A. J. Chromatogr. A, 2004, 1060: 23-41
[73] Liu Y, Grinberg N, Thompson K C, Wenslow R M, Neue U D, Morrison D, Walter T H, Gara J E O, Wyndham K D. Anal. Chim. Acta, 2005, 554: 144-151
[74] Neue U D. J. Sep. Sci., 2007, 30: 1611-1627
[75] Wu N, Liu Y, Lee M L. J. Chromatogr. A, 2006, 1131: 142-150
[76] Nguyen D T T, Guillarme D, Rudaz S, Veuthey J L. J. Sep. Sci., 2006, 29: 1836-1848
[77] Garcia-Canas V, Lorbetskie B, Girard M. J. Chromatogr. A, 2006, 1123: 225-232
[78] Klejdus B, Vacek J, Lojkova L, Benesova L, Kuban V. J. Chromatogr. A, 2008, 1195: 52-59
[79] Gosetti F, Frascarolo P, Polati S, Medana C, Gianotti V, Palma P, Aigotti R, Baiocchi C, Gennaro M C. Food Chem., 2007, 105: 1738-1747
[80] Pesek J J, Matyska M T, Sukul D. J. Chromatogr. A, 2008, 1191: 136-140
[81] Naeem H A, Sapirstein H. J. Cereal Sci., 2007, 46: 157-168
[82] Gritti F, Guiochon G. J. Chromatogr. A, 2007, 1166: 30-46
[83] Abrahim A, Al-Sayah M, Skrdla P, Bereznitsk Y, Chen Y, Wu N. J. Pharm. Biomed. Anal., 2010, 51: 131-137
[84] Lurie I S. J. Chromatogr. A, 2005, 1100: 168-175
[85] Klejdus B, Vacek J, Lojkova L, Lojkova L, Benesova L, Kuban V. J. Chromatogr. A, 2008, 1195: 52-59
[86] Liu Y, Grinberg N, Thompson K C, Wenslow R M, Neue U D, Morrison D, Walter T H, OGara J E, Wyndham K D. Anal. Chim. Acta, 2005, 554: 144-151
[87] Teutenberg T. Anal. Chim. Acta, 2009, 643: 1-12
[88] Nguyen D T T, Guillarme D, Heinisch S, Marie-Pierre B, Rocca J L, Rudaz S, Veuthey J L. J. Chromatogr. A, 2007, 1167: 76-84
[89] Lurie I, Li L. J. Liq. Chromatogr. Related Technol. , 2009, 32: 2615-2626
[1] 汤波, 王微, 罗爱芹. 新型多孔材料用作色谱手性固定相[J]. 化学进展, 2022, 34(2): 328-341.
[2] 史利娜, 胡欣, 朱宁, 郭凯. 纤维素基介电材料[J]. 化学进展, 2020, 32(12): 2022-2033.
[3] 吴亮, 沐春磊, 张群林*, 吕忱, 张晓悦. 纳米粒子参与的鲁米诺化学发光及其分析应用[J]. 化学进展, 2013, 25(07): 1187-1197.
[4] 钟亚兰 蒋序林. 高效液相色谱表征高聚物*[J]. 化学进展, 2010, 22(04): 706-712.
[5] 陈英 张锴 何锡文 张玉奎. 质谱技术鉴定细胞中组蛋白翻译后修饰的研究进展*[J]. 化学进展, 2010, 22(04): 713-719.
[6] 周瑛,叶丽,竹鑫平. HPLC-ICP-MS在食品中硒和砷形态分析及其生物有效性研究中的应用*[J]. 化学进展, 2007, 19(06): 982-995.
[7] 何永祝,庞浩,廖兵. 纤维素衍生物手性固定相研究进展[J]. 化学进展, 2006, 18(0708): 957-965.
[8] 敦惠娟,赵惠敏,陈立仁. 氧化锆基质HPLC 柱填料研究进展*[J]. 化学进展, 2005, 17(06): 1116-1122.
[9] 李来生,达世禄,冯钰锜,刘敏. 杯芳烃在液相色谱、毛细管电泳和电色谱中的应用进展**[J]. 化学进展, 2005, 17(03): 523-530.
[10] 蔡亚岐,刘稷燕,江桂斌. 固相微萃取-液相色谱联用技术研究进展*[J]. 化学进展, 2004, 16(05): 708-.