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化学进展 2012, Vol. Issue (9): 1656-1664 前一篇   后一篇

• 特约稿 •

基于电化学技术的microRNA生物传感器

闻艳丽1, 林美华1, 裴昊1, 鲁娜1,2, 樊春海*1   

  1. 1. 中国科学院上海应用物理研究所 上海 201800;
    2. 中国科学院上海微系统与信息技术研究所 上海 200050
  • 收稿日期:2012-03-01 修回日期:2012-06-01 出版日期:2012-09-24 发布日期:2012-09-27
  • 通讯作者: 樊春海 E-mail:fchh@ sinap.ac.cn
  • 基金资助:

    国家自然科学基金项目(No.20725516)资助

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导出 被引次数 ( 21 | 2 )

Electrochemical-Based MicroRNA Sensors

Wen Yanli1, Lin Meihua1, Pei Hao1, Lu Na1,2, Fan Chunhai1   

  1. 1. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;
    2. Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China
  • Received:2012-03-01 Revised:2012-06-01 Online:2012-09-24 Published:2012-09-27
MicroRNA(miRNA)是一种内源性的非编码单链RNA,通过与mRNA的3'端非翻译区(UTR)的不完全互补或完全互补结合抑制靶mRNA的翻译或促使靶mRNA的降解来调控基因的表达,参与细胞的增殖、凋亡、分化和代谢等重要过程。MiRNA表达的变化可以起到癌基因和抑癌基因的作用,是一种潜在的肿瘤标志物,因此,miRNA的检测技术引起了人们的关注。由于电化学检测方法具有灵敏、快速、低成本和低能耗等特点,研究者广泛开展了应用电化学技术来发展miRNA检测的研究。本文将对基于电化学技术的miRNA检测方法进行综述。
关键词： 电化学, microRNA, 生物传感器
MicroRNAs (miRNAs) are endogenous, non-coding single-stranded RNAs that regulate gene expression via degradation or translational repression of their targeted mRNAs. MiRNAs control cell growth, differentiation and apoptosis at a post-transciptional level. Recent evidence has shown that impaired miRNAs expression correlates with various human cancers and indicates that miRNAs can function as tumour suppressors and oncogenes, which provides a type of promising biomarkers for cancer diagnositics. Therefore, more and more researchers are interested in developing novel miRNAs detection methods. Because electrochemical methods are rapid, sensitive and electrochemical detectors are inexpensive and portable, there has been intense interest in developing new electrochemical sensors for miRNA detection. Here, studies on electrochemical miRNA biosensors are reviewed which contains the principle of DNA biosensor, several signal amplification technologies for biosensor, design of probe for miRNA biosensor and existing electrochemical methods for miRNA detection. Contents 1 Introduction
2 Principle and signal amplification technology for DNA biosensors
3 Design of capture probe for miRNA biosensor
3.1 Locked nucleic acids (LNA) probe
3.2 Peptide nucleic acids (PNA) probe
3.3 DNA nanostructural probe
4 MiRNA direct labeled detection technology
5 Label-free miRNA detection technology
5.1 Direct electrochemistry of miRNA based detection
5.2 Enzyme-based electrocatalytic amplification detection
5.3 Nano-electrode based detection
6 Conclusions and outlook
Key words: electrochemistry, microRNA, biosensors

中图分类号: 

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