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化学进展 2011, Vol. 23 Issue (8): 1737-1746 前一篇   后一篇

• 综述与评论 •

抗氧化能力评价方法

马小媛1,2, 钱卫平1*   

  1. 1. 东南大学生物科学与医学工程学院 生物电子学国家重点实验室 南京 210096;
    2. 东南大学化学化工学院 南京 210096
  • 收稿日期:2010-10-01 修回日期:2011-01-01 出版日期:2011-08-24 发布日期:2011-07-25
  • 通讯作者: 钱卫平 E-mail:wqian@seu.edu.cn
  • 基金资助:

    国家自然科学基金重大研究计划项目 (No.90923010)、国家重点基础研究发展计划(973)项目(No.2010CB933902)、国家科技部国际合作项目(No.2008DFB50060)和教育部高等学校博士学科点专项科研基金 (No.20090092110026)资助

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Methods to Determine Antioxidant Capacity

Ma Xiaoyuan1,2, Qian Weiping1*   

  1. 1. State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;
    2. School of Chemistry and Chemical Engineering, Southeast University, Nanjing 210096, China
  • Received:2010-10-01 Revised:2011-01-01 Online:2011-08-24 Published:2011-07-25

生物体系中的氧化与抗氧化系统失衡会导致氧化应激,被认为同诸多疾病的发病机理密切相关,具有抗氧化能力的物质能有效抵御氧化应激的有害损伤。本文介绍了氧化应激的有关概念,包括活性氧物种、抗氧化剂、氧化损伤等,并重点针对生物体内和体外的各种活性氧、活性氮物种清除能力检测方法以及本研究小组最新发展的利用金纳米壳复合材料和其他小组基于纳米材料的评价方法进行了综述与比较,包括化学原理、优缺点及实际应用等。简要分析了目前检测方法存在的问题并对未来标准化检测方法进行了展望。

关键词： 氧化应激, 抗氧化, 纳米材料, 检测方法

The imbalance between oxidants and antioxidants in biological systems may lead to oxidative stress, which is associated with the pathogenesis of several human diseases. The putative protective effects of antioxidants against these oxidative-induced reactions have received increasing attention. Here, the concept of oxidative stress is introduced, including reactive oxygen species, antioxidants, oxidative injury, etc. Furthermore, the methods most widely used for the determination of reactive oxygen species/reactive nitrogen species scavenging capacity as well as the newly developed evaluation assay using gold nanoshells composites by our research group and other methods based on nanomaterials are evaluated, presenting the general principles, recent applications, and their strengths and limitations. Finally, current problems and the prospect of the standardized analytical methods are proposed.

Contents
1 Introduction
2 Scavenging capacity assays against specific reactive oxygen species/reactive nitrogen species
2.1 ROO· scavenging capacity assay
2.2 O2·- scavenging capacity assay
2.3 H2O2 scavenging capacity assay
2.4 HO· scavenging capacity assay
2.5 HOCl scavenging capacity assay
2.6 1O2 scavenging capacity assay
2.7 NO· scavenging capacity assay
2.8 ONOO- scavenging capacity assay
3 Scavenging capacity assays against stable, non-biological radicals and evaluation of total reduction capacity
3.1 Trolox equivalent antioxidant capaity assay
3.2 DPPH radical scavenging capacity assay
3.3 Ferric reducing antioxidant power assay
3.4 Total reducing capacity estimated by electrochemical methods
4 Nanoparticles-based antioxidant capacity assay
4.1 Antioxidant capacity assay based on the growth process of gold nanoshells
4.2 Antioxidant capacity assay utilizing gold nanoparticles
4.3 Electrochemiluminescence sensors for HO· scavenging capacity assay based on CdSe quantum dots
4.4 Nanosensor for oxidative stress determination utilizing polymer spheres
4.5 Nanomaterials as antioxidants against harmful oxidative stress responses
4.6 Nanomaterials induced oxidative damage
5 Conclusions and outlook

Key words: oxidative stress, antioxidant, nanomaterials, detection method

中图分类号: 

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摘要

抗氧化能力评价方法