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化学进展 2011, Vol. 23 Issue (12): 2598-2606 前一篇   后一篇

• 综述与评论 •

电形成法制备脂质体

景婧1, 李轶1*, 刘剑1, 展思辉2*   

  1. 1. 天津大学理学院化学系 天津 30007;
    2. 南开大学环境科学与工程学院 天津 300071
  • 收稿日期:2011-04-01 修回日期:2011-08-01 出版日期:2011-12-24 发布日期:2011-09-29
  • 作者简介:e-mail:liyi@tju.edu.cn; sihuizhan@nankai.edu.cn
  • 基金资助:

    国家自然科学基金青年基金项目(No. 21003094, 20907022)、高等学校博士学科点专项科研基金项目(No. 20100032120066, 200800551003)和科技部科技人员服务企业项目(No. 2009GJA10021)资助

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Liposome Formation with Electroformation Method

Jing Jing1, Li Yi1*, Liu Jian1, Zhan Sihui2*   

  1. 1. Department of Chemistry, Chemistry Department of Tianjin University, Tianjin 30007;
    2. College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China
  • Received:2011-04-01 Revised:2011-08-01 Online:2011-12-24 Published:2011-09-29
脂质体具有类似生物膜的双分子层结构,利用电形成法制备的脂质体多为10—100μm的巨型单层囊泡,在光学显微镜下容易观察,因此利用电形成法制备的脂质体在模拟和替代细胞等方面的研究和应用具有较大潜力。本文对电形成法制备脂质体的研究进行了综述。文中介绍了传统的及改进后的电形成装置,对双分子层厚度、渗透压、电解质、固体表面、电压、频率、温度、pH值等过程参数对脂质体形成的影响作了详细阐述。
关键词： 脂质体, 磷脂分子, 电场, 电形成装置, 过程参数
Liposomes have bilayer structure,which is similar to that of bio-membrane.Most liposomes obtained by the electroformation method are giant unilamellar vesicles (diameter 10—100 μm), which can be observed under optical microscope easily.So they have great potential in simulation,replacement cells and other aspects. This paper reviews the influence of electric field on liposome formation and introduces the traditional and modified electroformation setup. We also elaborate the influence of process parameters on liposome formation, such as bilayer thickness, osmotic pressure, electrolyte, solid surface, voltage, frequency, temperature, pH value, etc. Contents 1 Introduction 2 Electroformation setup 2.1 Traditional electroformation setup 2.2 Modified electroformation setup 3 Effects of parameters on liposome formation 3.1 Effects of liposome materials 3.2 Effects of electricity factors 3.3 Effects of other factors 4 Conclusions
Key words: liposomes, phospholipids, electric field, electroformation setup, process parameters

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摘要

电形成法制备脂质体