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化学进展 2011, Vol. 23 Issue (10): 2003-2011 前一篇   后一篇

• 综述与评论 •

压力诱导溶液中蛋白质构象变化的谱学研究

张敏, 吴玉清*   

  1. 吉林大学超分子结构与材料国家重点实验室 长春 130012
  • 收稿日期:2011-01-01 修回日期:2011-03-01 出版日期:2011-10-24 发布日期:2011-09-15
  • 作者简介:e-mail:yqwu@jlu.edu.cn
  • 基金资助:

    国家自然科学基金项目(No.20973004,20473028)资助

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Spectroscopic Study on the Pressure-Induced Conformation Change of Protein in Aqueous Solution

Zhang Min, Wu Yuqing*   

  1. State Key Laboratory for Supramolecular Structure and Materials, Jilin University, Changchun 130012, China
  • Received:2011-01-01 Revised:2011-03-01 Online:2011-10-24 Published:2011-09-15

高压诱导蛋白质发生构象变化,引起蛋白质去折叠,并在某种条件下发生聚集,其结果是蛋白质原有的构造被破坏、发生变性。这些过程与蛋白质本身的结构密切相关。同时,折叠中间体结构的揭示对于研究蛋白质去折叠过程也具有重要意义。本文综述了高压诱导下多种谱学方法用于蛋白质构象变化的研究进展,主要包括高压红外光谱、高压荧光光谱及高压小角X射线扫描等多种技术手段在研究中蛋白质构象变化方面所能提供的重要信息,并对相关领域存在的问题及今后的发展作出展望。

关键词： 压力, 蛋白质, 水溶液, 光谱, 构象变化, 聚集

Pressure has been used for investigating the unfolding of proteins and for revealing their folding pathways and intermediates, even aggregate states. Since pressure can stabilize partially unfolded states and molten globules of proteins, which plays an important role both in chemical and biological phenomena in the aqueous solution. Here, we review several spectral methods developed for the study of pressure-induced conformation changes of proteins in solution, focus on infrared spectroscopy, fluorescence spectroscopy, small-angle X-ray scattering and so on, which can offer conformational information of proteins in solution in detail. The developing trends in this field are discussed and the outlook of the research area is also provided.

Contents
1 Introduction
2 FT-IR spectroscopic study on pressure-induced structure change of protein
2.1 Pressure-induced hydration change and reversibility of protein
2.2 Pressure-induced conformation transition of protein
2.3 Disulfide effect on pressure-induced conformation change of protein
2.4 Chaotropic and kosmotropic cosolvents effect on pressure-induced conformation change of protein
3 Fluorescence spectroscopic study on pressure induced conformation change of protein
4 Study of pressure induced conformation change of protein by small angle X-ray scattering and other methods
5 Conclusion and outlook

Key words: pressure, proteins, aqueous solution, spectroscopy, conformation change, aggregation

中图分类号: 

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