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化学进展 2010, Vol. 22 Issue (11): 2215-2223 前一篇   后一篇

• 综述与评论 •

毛细管电泳单细胞分析*

邓彬  徐见容  汪志明  肖玉秀**   

  1. (武汉大学药学院 武汉 430071)
  • 收稿日期:2010-03-02 修回日期:2010-06-18 出版日期:2010-11-24 发布日期:2010-10-20
  • 通讯作者: 肖玉秀 E-mail:yuxiuxiao@yahoo.com.cn
  • 基金资助:

    国家自然科学基金；高校基本科研业务费专项资金

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Recent Advances in Single-Cell Analysis Using Capillary Electrophoresis

Deng Bin   Xu Jianrong   Wang Zhiming   Xiao Yuxiu **   

  1. (College of Pharmacy, Wuhan University, Wuhan 430071, China)
  • Received:2010-03-02 Revised:2010-06-18 Online:2010-11-24 Published:2010-10-20
  • Contact: Xiao Yuxiu E-mail:yuxiuxiao@yahoo.com.cn

细胞是生命活动的基本单位，单细胞分析能够阐明细胞的结构与功能，揭示细胞之间的差异性，在细胞分化、生理病理研究及疾病早期诊断中具有重要作用。毛细管电泳作为一种高效的分离手段，所需样品体积小，能与高灵敏度检测器联用，特别适于单细胞分析。本文从单细胞进样技术、单细胞裂解技术、检测方法及应用等方面，对2007年以来毛细管电泳用于单细胞分析的最新进展做了相应叙述，并对未来的发展方向进行了展望。表明毛细管电泳不仅适于单细胞分析，而且仍有巨大的发展潜力和广阔的应用空间。

关键词： 毛细管电泳, 单细胞分析, 单细胞进样, 检测, 应用

Cells are the fundamental building blocks of life. Single-cell analysis can explain structures and functions of individual cells and uncovers the heterogeneity within populations of cells. It plays an important role in researches of cellular differentiation, physiology, pathology and early clinic diagnosis. As a high efficient separation method, capillary electrophoresis is uniquely suited for single-cell analysis because it needs ultrasmall samples and can be coupled  with sensitive detectors. The recent advances since 2007 in single-cell analysis using capillary electrophoresis are described, including single-cell injection techniques, single-cell lysis techniques, detection methods and applications. Future directions of single-cell analysis by capillary electrophoresis are also predicted. It is shown that capillary electrophoresis is not only suited for single-cell analysis but also still has tremendous development potentials and wide application fields.

Contents
1 Introduction
2 Single-cell injection techniques
3 Single-cell lysis techniques
4 Detection methods
4.1 Optical absorption-based detection
4.2 Laser-induced fluorescence
4.3 Chemiluminescence and electrochemiluminescence
4.4 Electrochemical detection
4.5 MS
5 Applications
5.1 Proteins and proteome
5.2 Metabolome
5.3 Subcellular structures
5.4 Neurotransmitters
5.5 Microorganisms
5.6 Others
6 Conclusions and outlook

Key words: capillary electrophoresis, single-cell analysis, single-cell injection, detection, applications

中图分类号: 

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摘要

毛细管电泳单细胞分析*