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化学进展 2010, Vol. 22 Issue (01): 140-147 前一篇   后一篇

• 综述与评论 •

人体呼出气中挥发性有机化合物的检测方法*

张晨;赵美萍**   

  1. (北京大学化学与分子工程学院 北京分子科学国家实验室(筹) 生物有机与分子工程教育部重点实验室  北京 100871)
  • 收稿日期:2009-02-26 修回日期:2009-04-23 出版日期:2010-01-24 发布日期:2010-01-07
  • 通讯作者: 赵美萍 E-mail:mpzhao@pku.edu.cn
  • 基金资助:

    国家自然科学基金资助项目

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Methods for Detection of Volatile Organic Compounds in Human Exhaled Breath

Zhang Chen;Zhao Meiping**   

  1. (College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China)
  • Received:2009-02-26 Revised:2009-04-23 Online:2010-01-24 Published:2010-01-07
  • Contact: Zhao Meiping E-mail:mpzhao@pku.edu.cn
  • Supported by:

    NSFC

呼出气体检测作为一种潜在的新型临床检测手段受到广泛关注。本文详细综述了人体呼出气中挥发性有机化合物（VOCs）的各类检测方法和技术，分别对色谱法、质谱法和光谱及传感器法的原理、特点和最新研究进展进行了介绍，对照总结了目前已确定的异戊二烯、丙酮等疾病生物标志物的各种分析方法和实测数据，并展望了未来的研究方向。

关键词： 人体呼出气体, 挥发性有机化合物（VOCs）, 检测方法, 生物标志物

Over hundreds of volatile organic compounds (VOCs) have been detected in human exhaled breath. Some of the organic constituents in exhaled breath can indicate a disease state of the individual or a recent exposure to an environmental pollutant. Determination of VOCs in human breath samples shows great potential in clinical diagnosis as a noninvasive method to replace or complement measurements from blood samples. This review focuses on the recent development of various methods for detection of VOCs in human exhaled breath. The advantages and limitations of different methods are highlighted. The analytical methods and concentration data of several important disease biomarkers such as isoprene and acetone are summarized. The future development trends in this field are also briefly discussed.

Contents
1 Introduction
2 Indirect analytical methods by gas chromatography
2.1 Sampling and storage
2.2 Preconcentration
2.3 Separation and detection by gas chromatography
3 Direct analytical methods by mass spectrometry
3.1 Selected ion flow tube mass spectrometry (SIFT-MS)
3.2 Proton transfer reaction mass spectrometry (PTR-MS)
3.3 Ion-molecule reaction mass spectrometry (IMR-MS)
4 Techniques based on spectroscopy, sensors and chemiluminescence
4.1 Spectroscopy
4.2 Electronic noses
4.3 Chemiluminescence
5 Conclusion

Key words: human exhaled breath, volatile organic compounds ( VOCs ), detection methods, biomarker

中图分类号: 

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