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化学进展 2010, Vol. 22 Issue (01): 133-139 前一篇   后一篇

• 综述与评论 •

磁场控制技术在微流控芯片中的应用*

沈玉勤;姚波**;方群**   

  1. （浙江大学化学系 微分析系统研究所 杭州 310058）
  • 收稿日期:2009-02-04 修回日期:2009-06-08 出版日期:2010-01-24 发布日期:2010-01-07
  • 通讯作者: 姚波;方群 E-mail:yaobo08@zju.edu.cn;fangqun@zju.edu.cn
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Application of Magnetic Control Technique in Microfluidic Chips

Shen Yuqin; Yao Bo**; Fang Qun**   

  1. （Institute of Microanalytical Systems, Department of Chemistry，Zhejiang University, Hangzhou 310058, China）
  • Received:2009-02-04 Revised:2009-06-08 Online:2010-01-24 Published:2010-01-07
  • Contact: Yao Bo;Fang Qun E-mail:yaobo08@zju.edu.cn;fangqun@zju.edu.cn

磁场作为除了电场和力场之外的另一个有力的驱动和控制手段，由于不需与溶液接触即可实现对被分析物的操纵，极大降低污染的可能，近年来被越来越广泛地用于微流控芯片系统，尤其在细胞、病毒甚至单分子的捕获、分选以及操纵等方面显示出较大优势。本文综述了微流控芯片系统中磁场控制技术的最新进展，分别从理论分析，磁场加工技术、泵阀的实现，微流体控制和磁分离等方面介绍了该领域近几年的发展状况，并重点分析了微流控芯片磁场操控技术在临床分析和现场检测方面的应用，及其未来发展趋势和需解决的主要问题。

关键词： 微流控芯片, 磁场控制, 生物分析

Magnetic force has become a promising tool for microfluid manipulation besides electrodynamic and hydrodynamic forces in microfluidic devices, because it can work under a non-contact mode without the limitations of the surface property of microchannels and the composition of working solutions, especially for capturing, sorting and manipulation of cells, virus and even single molecules. In this review, the recent developments within the field of magnetic microfluidics are presented including theory, fabrication of microcoils, design of magnetic pumps and valves, manipulation and separation based on magnetic control. The great potential of magnetic microfluidic systems in high throughput clinical diagnosis and point-of-care testing (POCT) is demonstrated and the difficulties for further application are also discussed.

Contents
1 Introduction
2 Fabrication of microcoils
3 Fluid control
3.1 Pump and valve
3.2 Mixing
4 Manipulation
4.1 Magnetic beads/cell manipulation
4.2 Micro droplets manipulation
5 Separation
5.1 Beads and cells sorting
5.2 Nucleic acids isolation
6 Bio assay
6.1 Immunoassay
6.2 Virus detection
6.3 Proteomics
6.4 NMR
7 Conclusion and prospects

Key words: microfluidic chip, magnetism control, bio-analysis

中图分类号: 

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