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化学进展 2010, Vol. 22 Issue (01): 125-132 前一篇   后一篇

• 综述与评论 •

智能聚合物的双水相体系在生物分子分离纯化中的应用*

屈锋**;吕锋华;张慧娟   

  1. (北京理工大学生命学院  北京 100081)
  • 收稿日期:2009-01-06 修回日期:2009-04-01 出版日期:2010-01-24 发布日期:2010-01-07
  • 通讯作者: 屈锋 E-mail:qufengqu@bit.edu.cn
  • 基金资助:

    国家重点基础研究发展计划（2007CB914101）；国家自然科学面上基金（20875009）

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Smart Polymers Based Aqueous Two-Phase Systems Applied in Bio-molecules Separation and Purification

Qu Feng**;  Lv Fenghua;  Zhang Huijuan   

  1. ( School of Life Science & Technology, Beijing Institute of Technology, Beijing 100081, China)
  • Received:2009-01-06 Revised:2009-04-01 Online:2010-01-24 Published:2010-01-07
  • Contact: Qu Feng E-mail:qufengqu@bit.edu.cn

简单地改变智能聚合物组成的双水相体系的外界条件，如温度、酸度、离子强度、光照强度、电场、磁场强度等，就可使成相聚合物与生物分子分离。基于智能聚合物的双水相体系分离过程简单，聚合物材料易于回收。近年来，以智能聚合物组成的双水相体系在分离纯化生物分子的研究中发展迅速。本文简要介绍了双水相萃取的原理，综述了十多年来温度敏感型、酸度敏感型、光敏型和具有亲和功能的双水相体系和双水相萃取与其他相关技术的结合在生物分子分离纯化中的研究进展。

关键词： 智能聚合物, 双水相体系, 生物分子, 分离纯化

Smart polymer based aqueous two-phase systems (ATPS) exhibit fast separation of target molecules with polymers and convenient recycle of polymers by simply changing environmental conditions such as temperature, pH, ionic strength and light or radiation forces. In recent years, the application of smart polymer based aqueous two-phase systems in separation and purification of bio-molecules is developing fast. In this paper, the principle of ATPS is introduced briefly. The applications of smart polymer based aqueous two-phase systems such as temperature-sensitive, pH-sensitive, photo-response, affinity functional, and combination of aqueous two-phase systems with related techniques in bio-molecule separation and purification in more than ten years are reviewed.

Contents
1 Introduction
2 The principle and the theoretical model of aqueous two-phase extraction
3 Studied of smart polymers based aqueous two-phase systems
3.1 Temperature-sensitive aqueous two-phase systems
3.2 pH-sensitive aqueous two-phase systems
3.3 Photo-response aqueous two-phase systems
3.4 Affinity aqueous two-phase systems
3.5 Combination of aqueous two-phase systems with related techniques
4 Prospects

Key words: smart polymers, aqueous two-phase systems, bio-molecules, separation and purification

中图分类号: 

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