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化学进展 2009, Vol. 21 Issue (11): 2397-2410 前一篇   后一篇

• 《化学进展》创刊20周年纪念专辑 •

生物芯片进展*

肖守军**;陈凌;许宁   

  1. (南京大学化学化工学院 配位化学国家重点实验室 南京微结构国家实验室 南京210093)
  • 收稿日期:2009-05-08 出版日期:2009-11-24 发布日期:2009-10-09
  • 通讯作者: 肖守军 E-mail:sjxiao@nju.edu.cn
  • 基金资助:

    国家自然科学基金资助项目

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Biochip Development

Xiao Shoujun**; Chen Ling; Xu Ning   

  1. (School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination Chemistry, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing 210093, China)
  • Received:2009-05-08 Online:2009-11-24 Published:2009-10-09
  • Contact: Xiao Shoujun E-mail:sjxiao@nju.edu.cn
  • Supported by:

    NSFC

生物芯片是近二十年来生物技术领域发展迅速和获得重大突破的高新技术，该综述介绍了经典的平铺生物芯片（DNA和蛋白质芯片）之后，重点介绍了近十年来发展的新的生物芯片的概念和内容：质谱为读出机制的表面增强激光解析离子化飞行时间质谱（SELDI）芯片、高通量DNA测序技术、基于胶体光子晶体的微球高通量生物检测技术、三维阵列生物芯片技术、和微流控等生物芯片技术。描述了各种生物芯片技术的优点、应用范围、和有待解决的问题，最后展望了生物芯片技术的未来。

关键词： 生物芯片, 读出机制, 蛋白质芯片, 三维阵列芯片, 微球高通量检测, 高通量DNA测序, 微流控生物芯片

Biochip is one of the most breakthrough technologies developed in the recent twenty years. In this review we first introduce the planar DNA and protein microarrays, then focus on the newly developed high technologies of SELDI (surface enhanced laser desorption/ionization) biochip, high throughput DNA sequencing, high throughput photonic crystal micro-bead bio-coding, three dimensional gel-pad biochip, and microfluidic biochip. We describe the advantages of each type of biochips, limits of its applications, and challenges to be solved. The future of biochip technologies will be prosperous.

Contents
1 Introduction
2 DNA microarray
2.1 Introduction of DNA miroarray
2.2 Preparation of DNA Microarray
3 Protein microarray
3.1 Introduction of protein microarray
3.2 Preparation of protein microarray
4 Biochip read-out mechanism
4.1 Fluorescence
4.2 Mass spectroscopy
4.3 Electrical read-out mechanism
5 SELDI mass protein biochip
6 High throughput DNA sequencing
7 High throughput photonic crystal micro-bead bio-coding
8 Three dimensional biochip
8.1 3-D gel-pad microarray
8.2 3-D porous silicon biochip
9 Microfluidic biochip
10 Outlook

Key words: biochip, read-out mechanism, protein microarray, three dimensional biochip, high throughput micro-bead bio-coding, high throughput DNA sequencing, microfluidic biochip

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[ 1 ] 　Stipp D. Fortune , 1997 , 135 : 56 —65
[ 2 ] 　Fodor S P , Read J L , Pirrung M C , et al . Science , 1991 , 251 :763 —773
[ 3 ] 　Fodor S P , Rava R P , Huang X C , et al . Nature , 1993 , 364 :555 —556
[ 4 ] 　Editorial , Nature Genetics , 1996 , 14 : 367 —370
[ 5 ] 　Sigal G B , Bamdad C , Barberis A. Analytical Chemistry , 1996 , 68 :490 —497
[ 6 ] 　Heyries K A , Marquette C A , Blum L J . Langmuir , 2007 , 23 :4523 —4527
[ 7 ] 　Lee J Y, Shah S S , Zimmer C C. Langmuir , 2008 , 24 : 2232 —2239
[ 8 ] 　Schena M. Microarray Analysis. 北京: 科学出版社(Beijing :Science Press) , 2004. 86
[ 9 ] 　Zhu H , Snyder M. Current Opinion in Chemical Biology , 2003 , 7 :55 —63
[10 ] 　Talapatra A , Rouse R , Hardiman G. Pharmacogenomics , 2002 , 3 :527 —536
[11 ] 　Hutchens T W, Yip T T. Rapid Commun. Mass Spectrom. , 1993 ,7 : 576 —580
[12 ] 　Ching J , Voivodov K I , Hutchens TW. J . Org. Chem. , 1996 , 61 :3582 —3583
[13 ] 　Mrksich M. ACS Nano , 2008 , 2 : 7 —18
[14 ] 　Margulies M, Egholm M, Rothberg J M, et al . Nature , 2005 , 437 :376 —380
[15 ] 　Rothberg J M, Leamon J H. Nature Biotechnology , 2008 , 26 :1117 —1124
[16 ] 　Shendure J , Porreca GJ , Reppas N B , et al . Science , 2005 , 309 :1728 —1732
[17 ] 　Zhao X W, Cao Y, Ito F , Gu Z Z, et al . Angew. Chem. Int . Ed. ,2006 , 45 : 6835 —6838
[18 ] 　Zhao Y J , Zhao X W, Hu J , Gu Z Z, et al . Adv. Mater. , 2009 ,21 : 569 —572
[19 ] 　Zhao Y J , Zhao X W, Gu Z Z, et al . Anal . Chem. , 2008 , 80 :1598 —1605
[20 ] 　Sun C , Zhao X W, Gu Z Z, et al . Small , 2008 , 4 : 592 —596
[21 ] 　Guschin D , Yershov G, Mirzabekov A , et al . Anal . Biochem. ,1997 , 250 : 203 —211
[22 ] 　Rubina A Y, Pan ’kov S V , Mirzabekov A D , et al . Anal .Biochem. , 2004 , 325 : 92 —106
[23 ] 　Rubina A Y, Dyukova V I , Dementieva E I , et al . Anal .Biochem. , 2005 , 340 : 317 —329
[24 ] 　Ressine A , Marko-Varga G, Laurell T. Biotechnol . Annu. Rev. ,2007 , 13 : 149 —200
[25 ] 　Laurell T, Nilsson J , Marko-Varga G. Anal . Chem. , 2005 , 77 :265 —272
[26 ] 　Davidsson R , Genin F , Bengtsson M, Laurell T. Lab on a Chip ,2004 , 4 : 481 —487
[27 ] 　Ressine A , Ekstrêm S , Marko2Varga G, Laurell T. Anal . Chem. ,2003 , 75 : 6968 —6974
[28 ] 　Zhou H , Xu S , Ye M, Feng S , Pan C , Jiang X, Li X, Han G, Fu Y, Zou H. J . Proteome Res. , 2006 , 5 : 2431 —2437
[29 ] 　Chen L , Chen Z T, Xiao S J , et al . Lab on a Chip , 2009 , 9 :756 —760
[30 ] 　Guo D J , Xiao S J , Xia B , et al . J . Phys. Chem. B , 2005 , 109 :20620 —20628
[31 ] 　Chen Y Q , Bi F , Xiao S J , et al . J . Chromat . B , 2008 , 875 :502 —508
[32 ] 　Yan H , Xu N , Xiao SJ , et al . Inter. J . Mass Spectrometry , 2009 ,281 : 1 —7
[33 ] 　Xia B , Xiao S J , Guo D J , et al . J . Mater. Chem. ,2006 , 16 :570 —578
[34 ] 　Thorsen T, Maerkl SJ , Quake S R. Science , 2002 , 298 : 580 —584
[35 ] 　Quake S R , Scherer A. Science , 2000 , 290 : 1536 —1540
[36 ] 　Unger M A , Chou H P , Thorsen T, Scherer A , Quake S R.Science , 2000 , 288 : 113 —116
[37 ] 　Zhu H , Snyder M. Curr. Opin. Chem. Biol . , 2003 , 7 : 55 —63
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生物芯片进展*