PDF(969 KB)
PDF(969 KB)
PDF(969 KB)
生命分析化学的重要领域——电化学微阵列芯片
- 许丹科; 陈洪渊*
作者信息
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Electrochemical Detection for Arrayed Electrode Chips
- Xu Danke; Chen Hongyuan*
Author information
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文章历史
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摘要
生命分析化学是研究与生命活动相关的化学分子检测原理与方法的新兴交叉科学领域。将电化学分析方法与生物芯片相结合形成的电化学生物芯片正在向集成化、通量化、微型化和自动化等方向迅速发展。本文介绍近年来电化学检测在DNA芯片、蛋白质芯片以及适配体芯片等方面有代表性的研究进展。文中阐述了高、低密度电化学DNA芯片的制备与多种伏安检测方法；讨论了多酶电极芯片结构与测定干扰之间的关系，介绍了伏安法、阻抗法等电化学方法在蛋白芯片中的应用进展。此外，还简要介绍了电化学适配体芯片的制备及检测方法，并展望了电化学生物芯片的发展前景。
Abstract
Ananlytical chemistry for life science is an interdisciplinary scientific field to develop novel analytical principles, methods and techniques for detecting chemical molecules and providing scientific data and information for life science. Combining electrochemical methods with microarray technology, electrochemical biochips are being accelerated towards integration, throughput, miniaturization, automation. Recent progresses in the development of electrochemical detection methods for DNA arrays and protein arrays as well as aptamer arrays are summarized in this review. In the case of electrochemical DNA arrays, numerous fabrication methods of DNA capture probes for high throughput and focused DNA arrays are presented. Electrochemical detection is focused on voltammetry by using various electrochemical probes or label-free approach. The development of arrayed electrodes immobilized with antibodies and aptamers is also discussed, in which voltammetry and electrochemical impedance measurements have been used to assay multiplexed protein targets.
Contents
1 Electrochemical detection for DNA chips
1.1 Focused DNA chips
1.2 High throughput DNA chips
2 Electrochemical detection for protein array chips
2.1 Enzyme arrayed electrodes
2.2 Electrochemical multiplex measurement for proteins
3 Aptamer arrays
关键词
电化学检测 / 微阵列芯片 / DNA芯片 / 蛋白质芯片 {{custom_keyword}} /
Key words
electrochemical detection / microarray chips / DNA chips / protein chips {{custom_keyword}} /
中图分类号：
O657.1
O629.7
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