English
往期目录
新闻公告
More
化学进展 2009, Vol. 21 Issue (05): 1052-1059 前一篇   后一篇

• 综述与评论 •

电化学DNA生物传感器研究的应用进展*

黄强;刘红英;方宾**   

  1. （安徽师范大学化学与材料科学学院 化学生物传感安徽省重点实验室 芜湖 241000）
  • 收稿日期:2008-05-28 修回日期:2008-08-19 出版日期:2009-05-24 发布日期:2009-05-05
  • 通讯作者: 方宾 E-mail:binfang_47@yahoo.com.cn
  • 基金资助:

    省级资助;国家自然科学基金

下载PDF ( 2482 ) 引用
导出

Development of Electrochemical DNA Biosensors

Huang Qiang; Liu Hongying; Fang Bin**   

  1. (Anhui Key Laboratory of Chemo-Biosensing, School of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China)
  • Received:2008-05-28 Revised:2008-08-19 Online:2009-05-24 Published:2009-05-05
  • Contact: Fang Bin E-mail:binfang_47@yahoo.com.cn

电化学DNA生物传感器因快速、灵敏、低耗和易于操作等优点在基因序列测定中受到了广泛的关注,已逐渐成为分子生物学和生物技术研究的重要领域。具有电活性的小分子和纳米材料因它们独特的性质，已被应用到电化学DNA生物传感器中。本文介绍了电化学DNA生物传感器的基本概念和分类，综述了近年来电活性小分子和纳米材料在电化学DNA生物传感器中的应用进展，并对此领域的未来发展做了展望。

关键词： 电化学DNA生物传感器, 电活性小分子, 纳米材料

Owing to the advantages of its high sensitivity, rapid response, easy handling, and low cost, the electrochemical DNA biosensor has received much attention in the field of genic sequence detection and has gradually become an important field in the study of molecular biology and biological research. Electroactive molecules and nano materials have been applied into the electrochemical DNA biosensors because of their special properties. Here, the principle and classification of the electrochemical DNA biosensors are introduced, and the applications of electroactive molecules and nano materials in electrochemical DNA biosensors in recent years are reviewed. A prospect for the future development in this field is also briefly given.

Contents
1 Principle of electrochemical DNA biosensors
2 The applications of electroactive molecules in electrochemical DNA biosensors
2.1 Non-labeling hybridization indicator as recognition element
2.2 Labeling hybridization indicator as recognition element
3 The applications of nano materials in electrochemical DNA biosensors
3.1 Use of metal nanoparticles
3.2 Use of semiconductor- and oxide- nanoparticles
3.3 Use of magnetic microspheres
3.4 Use of other nano materials
4 Outlook

Key words: electrochemical DNA biosensors, electroactive molecules, nano materials

中图分类号: 

()

[ 1 ] 　Vercoutere W, Akeson M. Cur. Opin. Chem. Biol . , 2002 , 6 :816 —822
[ 2 ] 　Thorp H H , March T. Trends Biotechnol . , 1998 , 16 : 117 —121
[ 3 ] 　Palecek E. Nature , 1960 , 188 : 656 —657
[ 4 ] 　马丽(Ma L) , 白燕(Bai Y) , 刘仲明(Liu Z M) , 刘芳(Liu F) .传感器技术(Journal of Transducer Technology) , 2002 , 21 : 58 —61
[ 5 ] 　汪庆祥(Wang Q X) , 袁显龙(Yuan X L) , 焦奎(Jiao K) , 谢江坤(Xie J K) , 张波(Zhang B) . 化学进展(Progress in Chemistry) ,2007 , 19 : 1007 —1015
[ 6 ] 　王振永(Wang Z Y) , 焦奎(Jiao K) , 孙伟(Sun W) , 李清军(Li Q J ) . 青岛科技大学学报(Journal of Qingdao University of Science and Technology(Natural Science Edition) ) , 2005 , 26 : 5 —8
[ 7 ] 　Kara P , Kagan K, Ozkan D , Meric B , Erdem A , Ozkan Z, Ozsoz M. Electrochem. Commun. , 2002 , 4 : 705 —709
[ 8 ] 　Lin X H , Wu P , Chen W, Zhang Y F , Xia X H. Talanta , 2007 ,72 : 468 —471
[ 9 ] 　Wang A H J , Ughetto G, Quigley G, et al . Biochemistry , 1987 ,26 : 1152 —1163
[10 ] 　Hashimoto K, Ito K, Ishimori Y, et al . Anal . Chem. , 1994 , 66 :3830 —3836
[11 ] 　Revenga-Parra M, García T, Lorenzo E , Pariente F. Biosens.Bioelectron. , 2007 , 22 : 2675 —2681
[12 ] 　Fang B , Jiao S , Li M, Qu Y, Jiang X. Biosens. Bioelectron. ,2008 , 23 : 1175 —1179
[13 ] 　Won B Y, Yoon H C , Park H G. Analyst , 2008 , 133 (1) : 100 —104
[14 ] 　Millan KM, Mikkelsen S R. Anal . Chem. , 1993 , 65 : 2317 —2323
[15 ] 　Garcia T, Revenga-Parra M, Abruna H D , Pariente F , Lorenzo E.Anal . Chem. , 2008 , 80 : 77 —84
[16 ] 　Korri-Youssoufi H , Bouchra M. Anal . Chim. Acta , 2002 , 469 :85 —92
[17 ] 　徐春(Xu C) , 蔡宏(Cai H) , 何品刚(He P G) , 方禹之(Fang Y Z) . 高等学校化学学报(Chem. J . Chin. Univ. ) , 2001 , 22 :1492 —1495
[18 ] 　Xiao Y, Qu X, Plaxco K W, Heeger A J . J . Am. Chem. Soc. ,2007 , 129 : 11896 —11897
[19 ] 　Lu Y, Li X, Zhang L , Yu P , Su L , Mao L. Anal . Chem. , 2008 ,80 : 1883 —1890
[20 ] 　Xiao Y, Lubin A A , Baker B R , et al . Proc. Natl . Acad. Sci .USA , 2006 , 103 : 16677 —16680
[21 ] 　徐春(Xu C) , 何品刚(He P G) , 方禹之(Fang Y Z) . 高等学校化学学报(Chem. J . Chin. Univ. ) , 2000 , 21 : 1187 —1190
[22 ] 　Kim K, Yang H , Park S H , Lee D , Kim S , Lim Y T, Kim Y T.Chem. Commun. , 2004 , 1466 —1467
[23 ] 　Willner I , Willner B. Pure Appl . Chem. , 2002 , 74 : 1773 —1783
[24 ] 　Ozsoz M, Erdem A , Kerman K, Ozkan D , Tugrul B , Topcuoglu N ,Ekren H , Taylan M. Anal . Chem. , 2003 , 75 : 2181 —2187
[25 ] 　Authier L , Grossiord C , Brossier P , Limoges B. Anal . Chem. ,2001 , 73 : 4450 —4456
[26 ] 　Kerman K, Morita Y, Takamura Y, Ozsoz M, Tamiya E. Anal .Chim. Acta , 2004 , 510 : 169 —174
[27 ] 　Cai H , Xu Y, Zhu N , He P , Fang Y. Analyst , 2002 , 127 : 803 —808
[28 ] 　Wang MJ , Sun C Y, Wang L Y, Ji X H , Bai YB , Li TJ , Li J H.J . Pharm. Biomed. Anal . , 2003 , 33 : 1117 —1125
[29 ] 　Karadeniz H , Erdem A , Caliskan A , Pereira C M, Pereira E M,Ribeiro J A. Electrochem. Commun. , 2007 , 9 : 2167 —2173
[30 ] 　Cai H , Wang Y, He P , Fang Y. Anal . Chim. Acta , 2002 , 469 :165 —172
[31 ] 　Lee TM H , Li L L , Hsing I M. Langmuir , 2003 , 19 : 4338 —4343
[32 ] 　Rochelet-Dequaire M, Limoges B , Brossier P. Analyst , 2006 , 131 :923 —929
[33 ] 　Zhang J , Song S P , Zhang L Y, Wang L H , Wu H P , Pan D , Fan C H. J . Am. Chem. Soc. , 2006 , 128 : 8575 —8580
[34 ] 　Li D , Yan Y, Wieckowska A , Willner I. Chem. Commun. , 2007 ,3544 —3546
[35 ] 　Moses S , Brewer S H , Kraemer S , Fuierer R R , Lowe L B , Agbasi C , Sauthier M, Franzen S. Sens. Actuators B , 2007 , 125 : 574 —580
[36 ] 　Cai H , Xu C , He P , Fang Y. J . Electroanal . Chem. , 2001 , 510 :78 —85
[37 ] 　Sun W, Zhong J , Zhang B , Jiao K. Anal . Bioanal . Chem. , 2007 ,389 : 2179 —2184
[38 ] 　Peng H , Soeller C , Cannell M B , Bowmaker G A , Cooney R P ,Travas-Sejdic J . Biosens. Bioelectron. , 2006 , 21 : 1727 —1736
[39 ] 　Hansen J A , Mukhopadhyay R , Hansen J O , Gothelf KV. J . Am.Chem. Soc. , 2006 , 128 : 3860 —3861
[40 ] 　Zhang D , Chen Y, Chen H Y, Xia X H. Anal . Bioanal . Chem. ,2004 , 379 : 1025 —1030
[41 ] 　Shrestha S , Yeung C M Y, Mills C E , Lewington J , Tsang S C.Angew. Chem. Int . Ed. , 2007 , 46 : 3855 —3859
[42 ] 　Erdem A , Sayar F , Karadeniz H , Guven G, Ozsoz M, Piskin E.Electroanalysis , 2007 , 19 : 798 —804
[43 ] 　Wang J , Xu D , Kawde A N , Polsky R. Anal . Chem. , 2001 , 73 :5576 —5581
[44 ] 　Wang J , Xu D , Polsky R. J . Am. Chem. Soc. , 2002 , 124 :4208 —4209
[45 ] 　Castaneda M T, Merkoci A , Pumera M, Alegret S. Biosens.Bioelectron. , 2007 , 22 : 1961 —1967
[46 ] 　Pumera M, Castaneda M T, Pividori M I , Eritja R , Merkoci A ,Alegret S. Langmuir , 2005 , 21 : 9625 —9629
[47 ] 　Liu H Y, Wang G F , Chen D L , Zhang W, Li I , Fang B. Sens.Actuators B. 2008 , 128 : 414 —421
[48 ] 　Wang J , Liu G, Rasul J M, Zhu Q. Electrochem. Commun. , 2003 ,5 : 1000 —1004
[49 ] 　Yang Y, Wang Z, Yang M, Li J , Zheng F , Shen G, Yu R. Anal .Chim. Acta , 2007 , 584 : 268 —274
[50 ] 　Zhu N N , Chang Z, He P G, Fang Y Z. Anal . Chim. Acta , 2005 ,545 : 21 —26
[51 ] 　Zhu N N , Cai H , He P G, Fang Y Z. Anal . Chim. Acta , 2003 ,481 : 181 —189
[52 ] 　Kawde A N , Wang J . Electroanalysis , 2004 , 16 : 1 —2
[53 ] 　Chang H , Yuan Y, Shi N , Guan Y. Anal . Chem. , 2007 , 79 :5111 —5115
[54 ] 　Wang Z, Yang Y, Leng K, Li J , Zheng F , Shen G, Yu R. Anal .Lett . , 2008 , 41 : 24 —35
[55 ] 　Pan J . Biochem. Eng. J . , 2007 , 35 : 183 —190
[56 ] 　Metfies K, Huljic S , Lange M, Medlin L K. Biosens. Bioelectron. ,2005 , 20 : 1349 —1357
[57 ]   Miranda-Castro R , de-los-Santos-Alvarez P , Lobo-Castanon M J ,Miranda-Ordieres A J , Tunon-Blanco P. Anal . Chem. , 2007 , 79 :4050 —4055
[58 ] 　Diaz-Gonzalez M, de la Escosura-Muniz A , Gonzalez-Garcia M B ,Costa-Garcia A. Biosens. Bioelectron. , 2008 , 23 : 1340 —1346
[59 ] 　Lin X H , Wan H Y, Zhang Y F , Chen J H. Talanta , 2008 , 74 :944 —950
[60 ] 　Berganza J , Olabarria G, Garcia R , Verdoy D , Rebollo A , Arana S.Biosens. Bioelectron. , 2007 , 22 : 2132 —2137
[1] 刘峻, 叶代勇. 抗病毒涂层[J]. 化学进展, 2023, 35(3): 496-508.
[2] 陆峰, 赵婷, 孙晓军, 范曲立, 黄维. 近红外二区发光稀土纳米材料的设计及生物成像应用[J]. 化学进展, 2022, 34(6): 1348-1358.
[3] 周晋, 陈鹏鹏. 二维纳米材料的改性及其环境污染物治理方面的应用[J]. 化学进展, 2022, 34(6): 1414-1430.
[4] 李彬, 于颖, 幸国香, 邢金峰, 刘万兴, 张天永. 手性无机纳米材料圆偏振发光的研究进展[J]. 化学进展, 2022, 34(11): 2340-2350.
[5] 郑明心, 谭臻至, 袁金颖. 光响应Janus粒子体系的构建与应用[J]. 化学进展, 2022, 34(11): 2476-2488.
[6] 漆晨阳, 涂晶. 无抗生素纳米抗菌剂:现状、挑战与展望[J]. 化学进展, 2022, 34(11): 2540-2560.
[7] 王嘉莉, 朱凌, 王琛, 雷圣宾, 杨延莲. 循环肿瘤细胞及细胞外囊泡的纳米检测技术[J]. 化学进展, 2022, 34(1): 178-197.
[8] 赵丹, 王昌涛, 苏磊, 张学记. 荧光纳米材料在病原微生物检测中的应用[J]. 化学进展, 2021, 33(9): 1482-1495.
[9] 谢勇, 韩明杰, 徐钰豪, 熊晨雨, 王日, 夏善红. 荧光内滤效应在环境检测领域的应用[J]. 化学进展, 2021, 33(8): 1450-1460.
[10] 程熙萌, 张庆瑞. 功能蛋白纳米材料在环境保护中的应用[J]. 化学进展, 2021, 33(4): 678-688.
[11] 谭莎, 马建中, 宗延. 聚(3,4-乙烯二氧噻吩)∶聚苯乙烯磺酸/无机纳米复合材料的制备及应用[J]. 化学进展, 2021, 33(10): 1841-1855.
[12] 蒋乔, 徐雪卉, 丁宝全. 纳米材料对生物凝聚态的调控[J]. 化学进展, 2020, 32(8): 1128-1139.
[13] 秦瑞轩, 邓果诚, 郑南峰. 金属纳米材料表面配体聚集效应[J]. 化学进展, 2020, 32(8): 1140-1157.
[14] 刘阳, 张新波, 赵樱灿. 二维MoS2纳米材料及其复合物在水处理中的应用[J]. 化学进展, 2020, 32(5): 642-655.
[15] 陈豪登, 徐建兴, 籍少敏, 姬文晋, 崔立峰, 霍延平. MOFs衍生金属氧化物及其复合材料在锂离子电池负极材料中的应用[J]. 化学进展, 2020, 32(2/3): 298-308.