English
往期目录
新闻公告
More
化学进展 2009, Vol. 21 Issue (0203): 503-513 前一篇   后一篇

• 环境化学专辑 •

DNA加合物检测*

冯峰;王超;吕美玲;汪海林**   

  1. (中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室　北京100085)
  • 收稿日期:2008-10-30 出版日期:2009-03-24 发布日期:2009-04-03
  • 通讯作者: 汪海林 E-mail:hlwang@rcees.ac.cn
  • 基金资助:

    973计划和前沿项目资助(No. 2007CB407305、2008CB417201);973项目

下载PDF ( 2377 ) 引用
导出

Detection and Quantification of DNA Adducts

Feng Feng; Wang Chao; LüMeiling; Wang Hailin**   

  1. (State Key Laboratory of Environmental Chemistry and Ecotoxicology , Research Center for Eco2Environmental Sciences , Chinese Academy of Sciences , Beijing 100085 , China)
  • Received:2008-10-30 Online:2009-03-24 Published:2009-04-03
  • Contact: Wang Hailin E-mail:hlwang@rcees.ac.cn

DNA加合物是一类极其重要的、具有多种标志作用的生物标志物，可应用于环境致癌物的暴露监测、基因毒性测定、个体敏感性和环境致癌物与基因的相互作用研究、癌症风险评价、环境致癌因素筛查以及癌症预防研究等。可靠、灵敏、准确的分析检测技术是DNA加合物作为生物标志物得到广泛应用的关键。当前DNA加合物分析、检测的方法主要包括：32P后标记法、免疫分析法、免疫毛细管电泳激光诱导荧光法、液相色谱串联质谱法、微分离-质谱联用法等。本文对这些分析技术和方法及其在DNA加合物检测方面的应用仅做一简要综述。

关键词： DNA加合物, 毛细管电泳激光诱导荧光, 32P后标记, 质谱

DNA adducts are an important class of biomarkers for monitoring of environmental carcinogen exposure and evaluation of population susceptibility, and an indicator of cancer risk. DNA adduct analysis has been widely applied in a number of fields, including human biomonitoring of carcinogen exposure, evaluation of genotoxicity and population susceptibility, carcinogen-gene interactions, cancer risk assessment, screening of carcinogenic environmental factors, cancer prevention. The reliable, sensitive and accurate detection and identification of DNA adducts is a key to the wide application of DNA adducts as biomarkers. A number of techniques and methods have been developed for the detection of DNA adducts, such as 32P-postlabelling, immunoassay, immuno-CE/LIF, LC/MS, micro-scale separation coupled MS. Applications of these techniques as well as related future development are briefly reviewed and discussed in this article.

Contents
1 Introduction
2 Analytical technologies for DNA adduct analysis
2.1 32P-postlabelling assays
2.2 Immunological assays
2.3 Capillary electrophoresis-laser induced fluorescence immunoassay
2.4 Liquid chromatography-tandem mass spectrometry
2.4.1 Brief on mass spectrometry
2.4.2 Sample pretreatment
2.4.3 Liquid chromatography-tandem mass spectrometric analysis
2.4.4 Application of LC-MS in DNA adduct analysis
2.5 Microbore and nano capillary LC-tandem mass spectrometry
3 The sensitivity and precision of DNA adduct analysis
4 Stereochemistry of DNA adducts and its analysis
5 Future perspectives

Key words: DNA adducts, capillary electrophoresis-laser induced fluorescence (CE-LIF), 32P-postlabelling, mass spectrometry

中图分类号: 

()

[ 1 ] 　Hemminki K. Arch. Toxicol. , 1983 , 52 : 249 —285
[ 2 ] 　Dipple A. Carcinogenesis , 1995 , 16 : 437 —441
[ 3 ] 　Denissenko M F , Pao A , Tang M, Pfeifer G P. Science , 1996 ,274 : 430 —432
[ 4 ] 　Smith L E , Denissenko M F , Tang M, et al . Journal of the National Cancer Institute , 2000 , 92 : 803 —811
[ 5 ] 　Nair U , Bartsch H , Nair J . Free Radical Biology & Medicine ,2007 , 43 : 1109 —1120
[ 6 ] 　Zhang F , Bartels M, Pottenger L , Gollapudi B , Schisler M. Mass Spectrom. , 2005 , 19 : 2767 —2772
[ 7 ] 　Siethoff C , Feldmann I , Jakubowski N , Linscheid M. J . Mass Spectrom. , 1999 , 34 : 421 —426
[ 8 ] 　Glover R P , Lamb J H , Farmer P B. Rapid. Commun. Mass Spec. ,1998 , 12 : 368 —372
[ 9 ] 　Doerge D R , Yi P , Churchwell M I , et al . Rapid Commun. Mass Spectrom. , 1998 , 12 : 1665 —1672
[10 ] 　Fichtinger-Schepman A MJ , van der Velder-Visser S D , van Dijk-Knijnenburg H C M, et al . Cancer Res. , 1990 , 50 : 7887 —7894
[11 ] 　Egner P A , Yu X, Johnson J K, Nathasingh C K, et al . Chem.Res. Toxicol . , 2003 , 16 : 1174 —1180
[12 ] 　Dennehy M K, Loeppky R N. Chem. Res. Toxicol . , 2005 , 18 :556 —565
[13 ] 　Van Delft J H , van den Ende A M, Keizer H J , et al .Carcinogenesis , 1992 , 13 : 1257 —1259
[14 ] 　Doerge D R , Churchwell M I , Fang J L , Beland F A. Chem. Res.Toxicol . , 2000 , 13 : 1259 —1264
[15 ] 　Da Costa G G, Churchwell M I , Hamilton L P , et al . Chem. Res.Toxicol . , 2003 , 16 : 1328 —1337
[16 ] 　Otteneder M, Lutz W K. Mutat . Res. , 1999 , 424 : 237 —247
[17 ] 　Randerath K, Reddy M V , Gupta R C. Proc. Natl . Acad. Sci .USA , 1981 , 78 : 6126 —6129
[18 ] 　Phillips D H. Handbook of Experimental Pharmacology. Berlin :Springer-Verlag , 1990. 503 —546
[19 ] 　Talaska G, Roh J H , Getek T. J . Chromatogr. , 1992 , 580 : 293 —323
[20 ] 　Beach A C , Gupta R C. Carcinogenesis , 1992 , 13 : 1053 —1074
[21 ] 　Phillips D H. Mutat . Res. , 1997 , 378 : 1 —12
[22 ] 　Hemminki K, Forsti A , Lofgren M, Segerback D , Vaca C , Vodicka P. IARC , Lyon. 1993. 51 —63
[23 ] 　Talaska G, Aljuburi A Z, Kadlubar F F. Proc. Natl . Acad. Sci .USA , 1991 , 88 : 5350 —5354
[24 ] 　Schmeiser H H , Bieter C A , Wiessler M, Cosyns J . Cancer Res. ,1996 , 56 : 2025 —2028
[25 ] 　Kuljukka2Rabb T, Peltonen K, Isotalo S , Mikkonen S , et al .Mutagenesis , 2001 , 16 : 353 —358
[26 ] 　Groopman J D , Donahue P R , Zhu J Q , et al . Proc. Natl . Acad.Sci . USA , 1985 , 82 : 6492 —6496
[27 ] 　Degan P , Shigenaga M K, Park EM, Alperin P E. Carcinogenesis ,1991 , 12 : 865 —871
[28 ] 　Rothman N , Poirier M C , Baser M E , Hansen J A , Gentile C , et al . Carcinogenesis , 1990 , 11 : 1241 —1243
[29 ] 　Santella R M, Hemminki K, Tang D L , Paik M, Ottman R. Cancer Epidemiol . Biom. Prev. , 1993 , 2 : 59 —62
[30 ] 　Santella R M. Cancer Epidemiology , Biomarkers and Prevention ,1999 , 8 : 733 —739
[31 ] 　Piece C P , Newman D J . Principles and Practice of Immunoassay ,New York : Stockton Press , 1991
[32 ] 　Schultz N M, Kennedy R T. Anal . Chem. , 1993 , 65 : 3161 —3165
[33 ] 　Le X C , Xing J Z, Lee J , Leadon S A , Weinfeld M. Science ,1998 , 280 : 1066 —1069
[34 ] 　Tan W G, Carnelley TJ , Le X C , et al . Journal of Chromatography A , 2001 , 924 : 377 —386
[35 ] 　Wang H , Lu M, Mei N , Lee J , Weinfeld M, Le X C. Anal . Chim.Acta , 2003 , 500 : 13 —20
[36 ] 　Wang H , Xing J , Tan W, LamM, Carnelley T, Weinfeld M, Le X C. Anal . Chem. , 2002 , 74 : 3714 —3719
[37 ] 　Wang H , Lu M, Weinfeld M, Le X C. Anal . Chem. , 2003 , 75 :247 —254
[38 ] 　Wang H , Lu M, Le X C. Anal . Chem. , 2005 , 77 : 4985 —4990
[39 ] 　Farmer P B , Lamb J , Lawley P D. IARC , Lyon. 1988. 347 —355
[40 ] 　Esmans E L , Broes D , Hoes I , Lemiere F , Vanhoutte K. J .Chromatogr. A , 1998 , 794 : 109 —127
[41 ] 　Singh R , McEwan M, Lamb J H , Santella R M, Farmer P B. Rapid Commun. Mass Spectrom. , 2003 , 17 : 126 —134
[42 ] 　Paehler A , Richoz J , Soglia J , Vouros P , Turesky RJ . Chem. Res.Toxicol . , 2002 , 15 : 551 —561
[43 ] 　Jones C R , Sabbioni G. Chem. Res. Toxicol . , 2003 , 16 : 1251 —1263
[44 ] 　Marsch GA , Botta S , Martin MV , McCormick WA , et al . Chem.Res. Toxicol . , 2004 , 17 : 45 —54
[45 ] 　Formenton2Catai A , Carrilho E. Anal . Bioanal . Chem. , 2003 ,376 : 138 —141
[46 ] 　Ravanat J L , Duretz B , Guiller A , Douki T, Cadet J . J .Chromatogr. B , 1998 , 715 : 349 —356
[47 ] 　Liao P , Li C , Hung C , Chen S. J . Mass Spectrom. , 2001 , 36 :336 —343
[48 ] 　Regulus P , Spessotto S , Gateau M, Cadet J , et al . Rapid Commun.Mass Spectrom. , 2004 , 18 : 2223 —2228
[49 ] 　Chernushevich I V , Loboda A V , Thomson B A. J . Mass Spectrom. , 2001 , 36 : 849 —865
[50 ] 　Zollner P , Zenker A , Galanski M, Keppler B K, Lindner W. J .Mass. Spectrom. , 2001 , 36 : 742 —753
[51 ] 　Deforce D , Lemiere F , Hoes I , et al . Carcinogenesis , 1998 , 19 :1077 —1086
[52 ] 　Harsch A , Sayer J M, Jerina D M, Vouros P. Chem. Res.Toxicol . , 2000 , 13 : 1342 —1348
[53 ] 　Tretyakova N , Matter B , Jones R , Shallop A. Biochemistry , 2002 ,41 : 9535 —9544
[54 ] 　Matter B , Wang G, Jones R , Tretyakova N. Chem. Res. Toxicol . ,2004 , 17 : 731 —741
[55 ] 　Tretyakova N , Matter B , Jones R , Shallop A. Biochemistry , 2002 ,41 : 9535 —9544
[56 ] 　Singh R , Gaskell M, le Pla R C , et al . Chem. Res. Toxicol . ,2006 , 19 : 868 —878
[57 ] 　Beland F A , Churchwell M I , von Tungeln L , et al . Chem. Res.Toxicol . , 2005 , 18 : 1306 —1315
[58 ] 　Casale G P , Singhal M, Bhattacharya S. Chem. Res. Toxicol . ,2001 , 14 : 192 —201
[59 ] 　Bhattacharya S , Barbacci D C , Shen M, Liu J , Casale G P. Chem.Res. Toxicol . , 2003 , 16 : 479 —486
[60 ] 　Chen L , Wang M, Villalta P W, Hecht S S. Chem. Res. Toxicol . ,2007 , 20 : 1498 —1502
[61 ] 　Podmore I D , Cooper D , Evans MD , Wood M, Lunec J . Biochem.Biophys. Res. Commun. , 2000 , 277 : 764 —770
[62 ] 　Jaruga P , Rodriguez H , Dizdaroglu M. Free Radical Biol . Med. ,2001 , 31 : 336 —341
[63 ] 　Frelon S , Douki T, Ravanat J L , Pouget J P , Torabene C , Cadet J .Chem. Res. Toxicol . , 2000 , 13 : 1002 —1007
[64 ] 　Singh R , Kaur B , Farmer P B. Chem. Res. Toxicol . , 2005 , 18 :249 —256
[65 ] 　Hakala K, Auriola S , Koivisto A , Lonnberg H. J . Pharm. Biomed.Anal . , 1999 , 21 : 1053 —1061
[66 ] 　Abian J , Oosterkamp A J , Gelpi E. J . Mass Spectrom. , 1999 , 34 :244 —254
[67 ] 　Hopfgartner G, Bean K, Henion J , Henry R. J . Chromatogr. ,1993 , 647 : 51 —61
[68 ] 　Bruins A P. J . Chromatogr. A , 1998 , 794 : 345 —357
[69 ] 　Willems A V , Deforce D L , van den Eeckhout E G, et al .Electrophoresis , 2002 , 23 : 4092 —4103
[70 ] 　Barry J P , Norwood C , Vouros P. Anal . Chem. , 1996 , 68 : 1432 —1438
[71 ] 　Gennaro L A , Vadhanam M, Gupta R C. Rapid Commun. Mass Spectrom. , 2004 , 18 : 1541 —1547
[72 ] 　Ding J , Vouros P. J . Chromatogr. A , 2000 , 887 : 103 —113
[73 ] 　Hoes I , Lemiere F , van Dongen W, Vanhoutte K, Esmans E L , et al . J . Chromatogr. B , 1999 , 736 : 43 —59
[74 ] 　Ricicki E M, Soglia J R , Teitel C , Kane R , Kadlubar F , Vouros P.Chem. Res. Toxicol . , 2005 , 18 : 692 —699
[75 ] 　Wang J J , Marshall W D , Law B , Lewis D M. Int . J . Mass Spectrom. , 2003 , 230 : 45 —55
[76 ] 　Wang J J , Marshall WD , Frazer D G, Law B , Lewis D M. Anal .Biochem. , 2003 , 322 : 79 —88
[77 ] 　Ricicki E , Luo W, Fan W, Zhao L , Zarbl H , Vouros P. Anal .Chem. , 2006 , 78 : 6422 —6432
[78 ] 　Vanhoutte K, van Dongen W, Hoes I , et al . Anal . Chem. , 1997 ,69 : 3161 —3168
[79 ] 　Embrechts J , Lemiere F , van Dongen W, et al . J . Am. Soc. Mass Spectrom. , 2003 , 14 : 482 —491
[80 ] 　Soglia J R , Turesky R J , Paehler A , Vouros P. Anal . Chem. ,2001 , 73 : 2819 —2827
[81 ] 　Beland F A , Doerge D R , Churchwell M I , Poirier MC , Schoket B ,Marques MM. Chem. Res. Toxicol . , 1999 , 12 : 68 —77
[82 ] 　Doerge D R , Churchwell M I , Marques M M, Beland F A.Carcinogenesis , 1999 , 20 : 1055 —1061
[83 ] 　Hu C W, Wu M T, Chao M R , et al . Rapid Commun. Mass Spectrom. , 2004 , 18 : 505 —510
[84 ] 　Chen H , Chiang L C , Tseng M C , Zhang L L , Ni J , Chung F L.Chem. Res. Toxicol . , 1999 , 12 : 1119 —1126
[85 ] 　Doerge D R , da Costa G G, Beland F A , et al . Mutat . Res. , 2005 ,580 : 131 —141
[86 ] 　Da Costa G G, Marques M M, Beland F A , et al . Chem. Res.Toxicol . , 2003 , 16 : 357 —366
[87 ] 　Yang S K, Gelboin H V. Biochem. Pharmacol . , 1976 , 25 : 2221 —2225
[88 ] 　Yang S K, McCourt D W, Roller P P , Gelboin H V. Proc. Natl .Acad. Sci . USA , 1976 , 73 : 2594 —2598
[89 ] 　Thakker D R , Yagi H , Levin W, Lu A Y H , Conney A H , Jerina D M. J . Biol . Chem. , 1977 , 252 : 6328 —6334
[90 ] 　Newbold R F , Brookes P. Nature , 1976 , 261 : 52 —54
[91 ] 　Huberman E , Sachs L , Yang S K, Gelboin H V. Proc. Natl . Acad.Sci . USA , 1976 , 73 : 607 —611
[92 ] 　Hsu I C , Bowden GT, Harris C C. Mutat . Res. , 1979 , 63 : 351 —359
[93 ] 　Slaga TJ , Bracken WJ , Gleason G, Levin W, Yagi H , Conney A H. Cancer Res. , 1979 , 394 : 67 —71
[94 ] 　Buening M K, Wislocki P G, Levin W, Yagi H , Thakker D R ,Akagi H , Koreeda M, Jerina DM, Conney A H. Proc. Natl . Acad.Sci . USA , 1978 , 11 : 5358 —5361
[95 ] 　Wood A W, Chang R L , Levin W, Yagi H , Thakker D R , Jerina DM, Conney A H. Biochem. Biophys. Res. Commun. , 1977 , 77 :1389 —1396
[96 ] 　Brookes P , Osborne M R. Carcinogenesis , 1982 , 3 : 1223 —1226
[97 ] 　Burgess J A , Stevens C W, Fahl W E. Cancer Res. , 1985 , 45 :4257 —4262
[98 ] 　Ruan Q , Kim H , Jiang H , Penning T, Harvey R , Blair I. Rapid Commun. Mass Spectrom. , 2006 , 20 : 1369 —1380
[99 ] 　Feng F , Yin J , Song M, Wang H. J . Chromatogr. A , 2008 , 1183 :119 —128
[1] 赵超, 蔡宗苇. 基于质谱成像和组学分析的环境毒理研究[J]. 化学进展, 2021, 33(4): 503-511.
[2] 王子璇, 厉欣, 再帕尔·阿不力孜. 化学衍生用于代谢物异构体质谱分析[J]. 化学进展, 2021, 33(3): 406-416.
[3] 杨笑宇, 贾珊珊, 张娟, 亓英华, 胡雪雯, 沈宝洁, 钟鸿英. 质谱光电离/解离技术和生物分子结构鉴定[J]. 化学进展, 2021, 33(12): 2316-2333.
[4] 蔡乐斯, 夏梦婵, 李展平, 张四纯, 张新荣. 二次离子质谱生物成像[J]. 化学进展, 2021, 33(1): 97-110.
[5] 李瑜玲, 赵君博, 郭寅龙. 常压电喷雾离子化的机理及应用[J]. 化学进展, 2019, 31(1): 94-109.
[6] 任娟, 边申, 王奕允, 孔祥蕾. 幻数团簇丝氨酸八聚体:结构和手性特征[J]. 化学进展, 2018, 30(4): 383-397.
[7] 顾芳婷, 胡敏*, 郑竞, 郭松. 大气颗粒物中有机硝酸酯的研究进展[J]. 化学进展, 2017, 29(9): 962-969.
[8] 刘婧靖, 何晓伟, 何燕, 喻目千, 蒋乐, 陈波. 纸喷雾敞开式质谱法的发展和应用[J]. 化学进展, 2017, 29(6): 659-666.
[9] 王方丽, 洪敏, 许丽丹, 耿志荣. 基于纳米材料的表面辅助激光解吸离子化质谱研究[J]. 化学进展, 2015, 27(5): 571-584.
[10] 田志美, 刘汪丹, 程龙玖. 硫醇保护金团簇的实验和理论研究现状[J]. 化学进展, 2015, 27(12): 1743-1753.
[11] 徐冲, 范艳璇, 范琳媛, 曹洁*, 胡长文*. 质谱在多金属氧簇化学中的应用[J]. 化学进展, 2013, 25(05): 809-820.
[12] 徐刚, 姜平元, 苟少华*. 抗肿瘤多核铂配合物的研究[J]. 化学进展, 2012, (9): 1707-1719.
[13] 储根柏, 陈军, 刘付轶*, 盛六四* . 气相自由基反应动力学的光电离质谱研究[J]. 化学进展, 2012, 24(11): 2097-2105.
[14] 彭晓敏, 张金超, 高愈希, 柴之芳. 金属蛋白的提取分离技术[J]. 化学进展, 2012, 24(05): 834-843.
[15] 张佳玲, 霍飞凤, 周志贵, 白玉, 刘虎威. 实时直接分析质谱的原理及应用[J]. 化学进展, 2012, 24(01): 101-109.
阅读次数
全文


摘要

DNA加合物检测*