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化学进展 2009, Vol. 21 Issue (01): 200-209 前一篇   后一篇

• 综述与评论 •

芯片毛细管电泳-安培检测系统*

王钰蓉;陈恒武**   

  1. (浙江大学化学系 微分析系统研究所 杭州 310058)
  • 收稿日期:2008-05-15 修回日期:2008-07-07 出版日期:2009-01-24 发布日期:2009-01-25
  • 通讯作者: 陈恒武 E-mail:hwchen@zju.edu.cn
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Microchip Capillary Electrophoresis with Amperometric Detection

Wang Yurong;Chen Hengwu**   

  1. (Institute of Micro Analytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China)
  • Received:2008-05-15 Revised:2008-07-07 Online:2009-01-24 Published:2009-01-25
  • Contact: Chen Hengwu E-mail:hwchen@zju.edu.cn

由于安培检测具有的高灵敏度、低成本、低能耗、易集成化便携化、与微加工技术匹配等特点，芯片毛细管电泳-安培检测系统（μCE-AD）的研究近年来得到人们广泛的关注。本文结合本课题组的研究工作，对近年来μCE-AD的研究进展进行评述；重点讨论了近年来在芯片的设计、集成化电极的制备、消除分离电压的干扰等方面的进展；同时介绍了利用分离电场拓展检测范围、阵列电极和阵列通道、化学修饰电极的应用、新型进样技术和试样预处理等方面的新成就；最后展望了未来μCE-AD的发展趋势。

关键词： 安培检测, 微流控芯片, 芯片毛细管电泳

Micochip capillary electrophoresis with amperometric detection (μCE-AD) has attracted increasing interest due to the features offered by amperometric detection, such as high sensitivity, low cost and power consumption, inherent miniaturization and portability, and compatibility with microfabrication technologies. This article reviews on recent advances in μCE-AD systems, including microchip design, fabrication of integrated electrodes, elimination of high-voltage interference with the detection. Field-non-isolating detection, electrode array and channel array, application of chemically modified electrodes, novel approaches for sample injection, and on-chip sample preparation are also discussed. The future development of the μCE-AD is foreseen.

Contents
1 Introduction
2 Design of μCE-AD chip
3 Fabrication of integrated electrodes
4 Elimination of high-voltage interference with the detection
5 Field-non-isolating detection
6 Electrode array and channel array
7 Chemically modified electrodes
8 Novel approaches for sample injection
9 On-chip sample preparation
10 Future prospects

Key words: amperometric detection, microfluidic chip, capillary electrophoresis chip

中图分类号: 

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摘要

芯片毛细管电泳-安培检测系统*