PDF(293 KB)
PDF(293 KB)
PDF(293 KB)
关于中药质量控制与体内代谢研究的思考
- 叶敏;果德安*
作者信息
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Strategies on the Quality Control and DMPK Studies of Traditional Chinese Medicine
- Ye Min; Guo Dean*
Author information
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文章历史
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摘要
质量控制是保证中药安全、有效的重要基础。体内代谢研究可以为阐明中药的治病机制提供依据。本文以蟾酥为例,讨论了当前中药质量控制及体内代谢研究存在的主要问题和解决办法:认为把中药指纹图谱的全面指认与多指标成分定量相结合是有效控制中药质量的可行途径;液相色谱2质谱联用技术(LCPMS) 将在中药化学研究、体内代谢研究等方面发挥重要作用;中药的化学指纹图谱应与药理活性相关联,从而建立合理的中药质量评价体系;结构修饰对基于天然产物的新药发现具有重要意义。
Abstract
Quality control is critically important for the safety and efficacy of traditional Chinese medicine. DMPK (drug metabolism and pharmacokinetic) studies facilitate the clarification of the mechanism of action of traditional Chinese medicine. In this paper , we discuss the current barriers in the quality control and DMPK studies of traditional Chinese medicine by using our studies on toad venom (Chan-Su) as example. It is concluded that the combination of full assignments of the chemical fingerprint and the simultaneous quantitation of multiple compounds is a feasible way for the comprehensive quality control of traditional Chinese medicines. Liquid chromatographyPmass spectrometry (LCPMS) will play an important role in chemistry and DMPK studies of traditional Chinese medicine. The chemical fingerprints of traditional Chinese medicines should be correlated with their pharmacological activities so as to establish a reasonable traditional Chinese medicine quality evaluation system. Structural modification could be valuable for natural product-based drug discovery.
Contents
1 Introduction
2 Studies on the traditional Chinese medicine Chan-Su (toad venom)
2. 1 Chemical constituents of toad venom
2. 2 Chemical fingerprint of toad venom and peak interpretation
2. 3 Quantitative analysis of major bufadienolides in toad venom
2. 4 In vivo metabolism of toad venom
2. 5 Structural modification of bufadienolides
3 Perspectives
3. 1 How to study the chemistry of traditional Chinese medicines ?
3. 2 How to establish the chemical fingerprint of traditional Chinese medicines ?
3. 3 How to study the DMPK of traditional Chinese medicines ?
3. 4 How to do the structural modification of natural products ?
4 Conclusions
关键词
中药 / 质量控制 / 体内代谢 / 指纹图谱 {{custom_keyword}} /
Key words
traditional Chinese medicines / quality control / DMPK studies / fingerprinting {{custom_keyword}} /
中图分类号：
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参考文献
[ 1 ] Ye M, Guo H , Guo H Z, et al . J . Chromatogr. B , 2006 , 838 (2) :86 —95
[ 2 ] Ye M, Guo D A. Rapid Commun. Mass Spectrom. , 2005 , 19 (13) :1881 —1892
[ 3 ] Liang Y, Liu A H , Qin S , et al . J . Pharm. Biomed. Anal . , 2008 ,46 : 442 —448
[ 4 ] Ye M, Dai J G, Guo H Z, et al . Tetrahedron Lett . , 2002 , 43(47) : 8535 —8538
[ 5 ] Ye M, Han J , Guo H Z, et al . Magn. Reson. Chem. , 2002 , 40(12) : 786 —788
[ 6 ] Ye M, Ning L L , Zhan J X, et al . J . Mol . Catal . B : Enzym. ,2003 , 22 (1P2) : 89 —95
[ 7 ] Ye M, Han J , Tu G Z, et al . J . Nat . Prod. , 2005 , 68 ( 4) :626 —628
[ 8 ] Ye M, Han J , An D G, et al . Tetrahedron , 2005 , 61 ( 37) :8947 —8955
[ 9 ] Ye M, Guo D A. Nat . Prod. Res. , 2008 , 22 (1) : 26 —30
[10 ] Ye M, Qu GQ , Guo H Z, et al . Appl . Environ. Microbiol . , 2004 ,70 (6) : 3521 —3527
[11 ] Ye M, Guo D A. J . Biotechnol . , 2005 , 117 (3) : 253 —262
[12 ] Ye M, Qu GQ , Guo H Z, et al . J . Steroid Biochem. Mol . Biol . ,2004 , 91 (1P2) : 87 —98
[13 ] Ye M, Yan Y N , Guo D A , et al . Rapid Commun. Mass Spectrom. , 2005 , 19 (11) : 1469 —1484
[14 ] Ye M, Han J , Chen H B , et al . J . Am. Soc. Mass Spectrom. ,2007 , 18 (1) : 82 —91
[15 ] Ye M, Guo D A , Ye G, et al . J . Am. Soc. Mass Spectrom. ,2005 , 16 (2) : 234 —243
[16 ] Ma X C , Cui J , Zheng J , et al . J . Mol . Catal . B : Enzym. , 2007 ,48 : 42 —50
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