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化学进展 2008, Vol. 20 Issue (11): 1761-1767 前一篇   后一篇

• 综述与评论 •

荧光和圆二色谱法用于低密度脂蛋白氧化的研究

孙霞 刘庆平 郑学仿   

  1. 大连大学生物工程学院 大连大学生物工程学院
  • 收稿日期:2007-11-30 修回日期:2008-02-29 出版日期:2008-11-24 发布日期:2008-11-25
  • 通讯作者: 刘庆平

Studies of Oxidation of Low Density Lipoprotein with Flourescence and Circular Dichroism Spectroscopy

孙霞 SUN Xia   

  • Received:2007-11-30 Revised:2008-02-29 Online:2008-11-24 Published:2008-11-25

氧化修饰低密度脂蛋白(oxidized low density lipoprotein,oxLDL)被认为是动脉硬化的关键致病因素。oxLDL的性能与其唯一的组成蛋白—载脂蛋白B-100(apoB-100)的二级结构即构象密切相关。荧光光谱和圆二色(circular dichroism,CD)光谱是研究蛋白质构象的有力手段,因此在oxLDL结构的研究中也有广泛的应用。本文综述了近年来荧光光谱和圆二色光谱在LDL氧化研究方面的应用, 并对今后的研究方向做了展望。

Oxidized low density lipoprotein(oxLDL) is thought to be a key pro-atherogenic event. The function of oxLDL is highly related to the secondary structure , i . e. the conformation of only constitutive protein—apoliprotein B-100(apoB-100). Both florescence and circular dichroism (CD) spectroscopy are the powerful tools for the investigation of the protein conformations , so they are widely used in the study of apoB-100 as well . In this review , the research of the structures on oxLDL by both fluorescence and CD spectroscopy are summarized and the future studies are prospected.

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