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化学进展 2008, Vol. 20 Issue (06): 975-983 前一篇   后一篇

• 综述与评论 •

蛋白质中二硫键的定位及其质谱分析*

仇晓燕1,2 　崔勐1 刘志强1 刘淑莹1**   

  1. (1. 中国科学院长春应用化学研究所 长春质谱中心 长春 130022;
    2. 中国科学院研究生院 北京 100039)
  • 收稿日期:2007-07-31 修回日期:2007-09-17 出版日期:2008-06-24 发布日期:2008-06-24
  • 通讯作者: 刘淑莹
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Protein Disulfide Bond Determination and Its Analysis by Mass Spectrometry

Qiu Xiaoyan1,2　 Cui Meng1 Liu Zhiqiang1 Liu Shuying1**   

  1. (1. Changchun Center of Mass spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; 2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China)
  • Received:2007-07-31 Revised:2007-09-17 Online:2008-06-24 Published:2008-06-24
  • Contact: Shuying Liu
二硫键是一种常见的蛋白质翻译后修饰，对稳定蛋白质的空间结构，保持及调节其生物活性等都有着非常重要的作用。因此，确定二硫键在蛋白质中的位置是全面了解含二硫键蛋白化学结构的重要方面。在众多实验方法中，现代质谱技术因其操作简单、快速、灵敏等优点而成为分析二硫键的重要手段。本文介绍了目前主要的定位二硫键的方法，以及质谱在二硫键定位分析中的应用与进展。
关键词： 二硫键定位, 质谱, 串联质谱, 三羧乙基膦, 稳定同位素标记

Disulfide bonds are one of the most common covalent posttranslational modifications of proteins. They play an important role in maintaining the three-dimensional structures of proteins, and their biological activities. Therefore, the determination of disulfide bonds becomes an important aspect of obtaining a comprehensive understanding of the chemical structure of a protein. Numerous experimental methods have been developed for the determination of disulfide bonds in proteins. Modern mass spectrometry has developed as an important tool for the analysis of disulfide bond patterns due to its advantages of being simple, rapid and sensitive. Some useful methods for assignment of disulfide bonds in proteins are introduced, the developments and applications of mass spectrometry in this area are reviewed.

Key words: assignment of disulfide bond, mass spectrometry, MS/MS, TCEP, Stable isotope labeling

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