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化学进展 2007, Vol. 19 Issue (05): 820-832 前一篇   

• 综述与评论 •

微流控分析芯片上生化反应技术研究进展*

徐溢1,2**;吕君江1;范伟1;温志渝2   

  1. 1.重庆大学化学化工学院 重庆 400044;

    2.重庆大学光电工程学院微系统研究中心 重庆 400044

  • 收稿日期:2006-03-28 修回日期:2006-05-19 出版日期:2007-05-24 发布日期:2007-05-24
  • 通讯作者: 徐溢
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Progress of Biochemical Reaction Technologies on Microfluidic Chips

Xu Yi1,2**;Lv Junjiang1;Fan Wei1;Wen Zhiyu2   

  1. 1. College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044;

    2.School of Optoelectronic Engineering, Chongqing University,Chongqing 400044,China

  • Received:2006-03-28 Revised:2006-05-19 Online:2007-05-24 Published:2007-05-24
综述了近几年来在微流控芯片上以检测或动力学研究为目的而进行的各种生物化学反应技术，包括酶反应、免疫反应、酶联免疫反应、PCR反应，含氮氧化物释放反应。对非均相反应的蛋白质在微管道中的固定技术，均相反应的管道设计技术，以及在微流控芯片上集成生化反应的优越性等问题做了详细的讨论。
关键词： 酶反应, 免疫反应, 酶联免疫反应, 聚合酶链反应, 微流控芯片
Different kinds of biochemical reaction approaches for the goal of detection and kinetics studies on microfluidic chips are reviewed, including enzymatic assay, immunoassay, enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) and nitric oxide release reaction. According to the very characteristics of certain derivative reaction, immobilization of proteins on channel wall for inhomogeneous reactions, channel network design for homogeneous reactions and as well the advantages of performing biochemical reaction on microfluidic chips are investigated in details
Key words: enzymatic assay, immunoassay, ELISA, PCR, microfluidic chip

中图分类号: 

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