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化学进展 2006, Vol. 18 Issue (05): 627-634 前一篇   后一篇

• 综述与评论 •

DNA体外缩合的研究进展

张炳琦 刘文广 唐聪 吉伟航 姚康德   

  1. 天津大学高分子材料研究所 天津大学高分子材料研究所 天津大学高分子材料研究所
  • 收稿日期:2005-07-01 修回日期:1900-01-01 出版日期:2006-01-01 发布日期:2006-01-01

Advances in the Study of DNA Condensation in Vitro

Bingqi Zhang Wenguang Liu Cong Tang Weihang Ji Kangde Yao   

  1. Institute of Polymeric Materials, Tianjin University Institute of Polymeric Materials, Tianjin University Institute of Polymeric Materials, Tianjin University
  • Received:2005-07-01 Revised:1900-01-01 Online:2006-01-01 Published:2006-01-01
DNA缩合不仅是自然界常见的生理现象,也是非病毒载体介导基因转染的关键步骤。了解DNA体外缩合的性质与特征,将有助于设计出高效的非病毒转基因载体。本文综述了近十几年来以多价阳离子为缩合剂诱发的DNA体外缩合的研究进展,着重介绍了缩合的机理、过程、影响缩合的因素以及应用于转基因的最新成果。
DNA condensation is a common physiological phenomenon in nature and also a key step in non-viral vector-mediated gene transfection. Understanding the characteristics of DNA condensation in vitro will aid in designing highly efficient non-viral vectors. In this article, the latest progress in the study of DNA condensation in vitro induced by multivalent cations is reviewed covering the mechanism, process, influencing factors of DNA condensation and its recent applications in gene transfection.

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[1] 陈晓东,姜思光,刘鸣华. DNA分子器件*[J]. 化学进展, 2003, 15(04): 332-.
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摘要

DNA体外缩合的研究进展