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化学进展 2005, Vol. 17 Issue (03): 482-498 前一篇   后一篇

• 综述与评论 •

微流控芯片技术在生命科学研究中的应用*

王立凯;冯喜增**   

  1. 南开大学生物活性材料教育部重点实验室 天津 300071
  • 收稿日期:2004-04-01 修回日期:2004-08-01 出版日期:2005-05-24 发布日期:2005-05-24
  • 通讯作者: 冯喜增
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Microfluidic Network for Research and Application in Life Sciences

Wang Likai;Feng Xizeng**   

  1. The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University,Tianjin 300071,China
  • Received:2004-04-01 Revised:2004-08-01 Online:2005-05-24 Published:2005-05-24
  • Contact: Feng Xizeng
微流控芯片最初起源于分析化学领域,是一种采用精细加工技术,在数平方厘米的基片,制作出微通道网络结构及其它功能单元,以实现集微量样品制备、进样、反应、分离及检测于一体的快速、高效、低耗的微型分析实验装置.随着微电子及微机械制作技术的不断进步,近年来微流控芯片技术发展迅猛,并开始在化学、生命科学及医学器件等领域发挥重要作用.本文首先简单介绍了微流控芯片制作材料和工艺,然后主要阐述了其在蛋白质分离、免疫分析、DNA分析和测序、细胞培养及检测等方面的应用进展.
关键词： 微流控芯片, 免疫分析, DNA分析, 细胞培养
Microfluidic chip technology originated from analytical chemisLry, adopts microfabrication technologies to make microchannels on a chip about several square centimeters. The technology can integrate the sample's injection, separation and detection into a single chip. The advantage of microfluidics is rapid, high efficiency and low consumption. With the progress of microelectronics and other microfabrication techniques, the technology of microfluidic chip developed rapidly recent years, and began to play more and more important roles in chemistry, biology and medical instruments. This article introduced the materials and techniques of manufacturing a microfluidic chip first, and then mainly expounded its applications in protein separation and analysis, immunoassay, DNA analysis and detection, and cell culture and detection.
Key words: microfluidic chip, immunoassay, DNA analysis, cell culture

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