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化学进展 1995, Vol. 7 Issue (02): 83-   后一篇

• 综述与评论 •

免疫分析法进展

杨晓达 常文保 慈云祥*   

  1. （北京大学化学与分子工程学院 北京 100871）
  • 收稿日期:1994-12-01 修回日期:1900-01-01 出版日期:1995-06-01 发布日期:1995-06-01
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Immunoassay: A Comprehensive Review and Prospect

Yang Xiaoda; Chang Wenbao; Ci Yunxiang   

  1. (College of Chemistry $. Molecular Engineering, Peking University, Beijing 100871)
  • Received:1994-12-01 Revised:1900-01-01 Online:1995-06-01 Published:1995-06-01

本文首先从试剂分析的观点对免及分析的原理进行了论.述，对抗体作为分析试剂进行了评价并且总结了标记免投分析的三种方式和四个环节;其次，对免疫分析的现状作了综述，并评述了当代免反分析的五个热点:基因工程抗体，生物素一亲合素多重标记体系，时间分辨荧光免疫分析，多组分免疫分析和自动化免疫分析;最后，对免疫分析的发展趋势作了讨论。

关键词： 免疫分析;抗体;基因工程抗体;多重标记;时间分辨荧光免疫分析

In this paper，the principle of immunoassay is restated in terms of reagent analysis. Three types and four key links of label immunoassay are discussed，and some commenu are made on antibody as an analytical reagent. The current situation of immunoassays is reviewed and five hot topics of nowaday immunoassays are. introduced，they are:recombination antibody，biotin-aviding labeling system，time-resolved fluoroimmunoassay，multianalyte immunoassay，and automatic immunoassay. The trend of immunoassay is also discussed.

Key words: immunoassay;antibody;gene-engineering Ah;multilabeling;TR一FIA
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摘要

免疫分析法进展