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化学进展 DOI: 10.7536/PC240304   

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细胞内单链DNA及高通量分析技术研究进展

李锐奇1,2, 赖玮毅1, 汪海林1,2,*   

  1. 1.中国科学院生态环境研究中心 北京 100085;
    2.国科大杭州高等研究院 杭州 310024
  • 收稿日期:2024-03-04 修回日期:2024-03-30
  • 基金资助:
    国家自然科学基金项目（No.22234008,21927807,22021003,22274166）资助
引用
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Advances in intracellular single strand DNA research and high-throughput analysis techniques

Ruiqi Li1,2, Weiyi Lai1, Hailin Wang1,2,*   

  1. 1. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;
    2. Hangzhou Institute for Advance Study, University of Chinese Academy of Sciences, Hangzhou 310024, China
  • Received:2024-03-04 Revised:2024-03-30
  • Contact: *e-mail: hlwang@rcees.ac.cn
  • Supported by:
    National Natural Science Foundation of China (No. 22234008,21927807,22021003,22274166).
在DNA复制、转录、双链断裂损伤修复等生命过程中,双链DNA会暂时解旋形成单链DNA（single strand DNA, ssDNA）。ssDNA可能影响基因组的稳定性,也可能参与构成非B型结构DNA,反过来调节和影响某些关键细胞和有机体过程。本综述简要介绍了单链DNA形成的原因、参与构成的结构及在细胞中可能产生的功能,并总结了部分单链DNA高通量分析技术,为后续单链DNA研究提供方法启示,促进单链DNA分析技术和方法的进一步发展。
关键词： 单链DNA, R环, G-四链体, 高通量测序
During many life processes such as replication, transcription, and double-strand breaks repair and so on, double-stranded DNA will temporarily unwind and form single strand DNA (ssDNA). ssDNA may affect genomic stability and may also participate in the formation of non-B DNA structure, which in turn regulates and influences many key cellular processes. This review briefly describes the causes of the formation of single-stranded DNA, the structures containing single-stranded DNA and their possible functions in cells, and summarizes some high-throughput analysis techniques of single-stranded DNA, which provides the method inspiration for the subsequent ssDNA research and promotes the further development of ssDNA analysis techniques and methods．
Key words: single strand DNA, R-loop, G-quadruplex, high-throughput sequencing
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