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化学进展 2023, Vol. 35 Issue (10): 1492-1504 DOI: 10.7536/PC230301 前一篇   后一篇

• 综述 •

金属组学和金属蛋白质组学技术于生物医药研究的应用

王宇传*()   

  1. 华北理工大学基础医学院 河北省慢性疾病重点实验室 唐山 063210
  • 收稿日期:2023-03-07 修回日期:2023-04-09 出版日期:2023-10-24 发布日期:2023-06-12
  • 作者简介:
    王宇传 香港大学博士,先后于中山大学化学学院和中国科学院深圳先进技术研究院任副研究员。在金属组学方法开发、细胞内金属蛋白鉴定、金属蛋白功能及金属药物作用机理研究等方面做出了系统的研究工作。以第一或共同通讯作者发表学术论文10余篇,主持或作为课题骨干参加多项国家级课题。研究方向为生物样品中金属元素的检测分析、生物金属机理探究等。
  • 基金资助:
    河北省高校基本科研业务费(JQN2022026)
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Applications of Metallomics and Metalloproteomics Techniques in Biomedical Research

Yuchuan Wang*()   

  1. Heibei Key Laboratory for Chronic Diseases, School of Basic Medical Sciences, North China University of Science and Technology,Tangshan 063210, China
  • Received:2023-03-07 Revised:2023-04-09 Online:2023-10-24 Published:2023-06-12
  • Contact: *e-mail: wangych@ncst.edu.cn
  • Supported by:
    Fundamental Research Funds for the Central Universities(JQN2022026)

金属是生命过程中必不可少的辅助因子,是许多关键细胞进程中的必需元素。金属组学作为一门新兴的研究领域,旨在了解并揭示基于金属的生命过程的分子机制及金属的生物活性,相关研究在近年来得以蓬勃发展并受到广泛关注。本文详述了金属组学的概念及相关研究技术,重点介绍金属组学的一个重要研究分支——金属蛋白质组学,并对该领域应用于生物医药研究取得的进展进行综述,内容涵盖金属/金属药物在单细胞层面的摄取研究,组织和器官中的金属/金属药物分布研究、及其在细胞内结合靶点蛋白的鉴定及表征,金属蛋白的生物信息学分析等方面。基于以上研究现状,进一步探讨了金属组学技术在生物医药研究中所面临的挑战及发展前景。

关键词： 金属组学, 金属蛋白质组学, 金属药物, 药物靶点蛋白

Metals are recognized as essential cofactors in life processes and are fundamental elements in many key cellular processes. Metallomics, as an emerging research field, aims to understand and reveal the functions of bio-active metals and the molecular mechanisms of metal-based life processes, and the related studies have received growing attention due to its rapid development as a frontier science. In this review, we first introduce the concept of metallomics and the related research technologies, and focuses on an important research branch in this field, metalloproteomics, which aims to recognize the relationships between biometals and cellular proteins in a systematic manner. The development of this field has provided a number of practical research tools. We summarize and highlight the recent applications, major progress and important research findings of metallomics and metalloproteomics in biomedical research, which cover the studies of metals/metallodrugs uptake at the single-cell level, the distributions of metals/metallodrugs in cells, tissues and organs, the identification and characterization of intracellular metal-binding proteins, as well as the bioinformatics analysis of metalloproteins. Based on the current research status, the challenges and prospects of the applications of metallomics techniques in biomedical research are further discussed. Moreover, popularization of the metalloproteomics research would be an innovate and efficient way to obtain a complete understanding of the role of bioactive metals in cells. We believe that the development of new methodologies in metallomics and metalloproteomics, as well as the discovery of novel metal-related biological mechanisms will facilitate, support and expand the research perspectives in biomedicine and clinical research.

Contents

1 Introduction

2 Metallomics and metalloproteomics

2.1 Definition

2.2 Research methods and techniques

3 Applications and progress in biomedical research

3.1 Metals/metallodrugs uptake studies at single cell levels

3.2 Distribution studies of metals/metallodrugs in cells and tissues

3.3 Identification of metallodrug-targeting proteins in cells

4 Conclusion and outlook

Key words: metallomics, metalloproteomics, metallodrugs, drug-targeting proteins
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图1 金属蛋白质组学方法及应用。(A) 由ICP-MS和LC-MS/MS技术组成的传统整合分析方法;(B) 大肠杆菌胞浆中与Ag+相关的蛋白质图谱;(C)固定化金属亲和色谱法(IMAC);(D) 药物下拉实验的工作流程
Fig.1 Illustration of metalloproteomics approaches and the applications. (A) Conventional integrated analytical methods consisted of ICP-MS and LC-MS/MS; (B) Map of Ag+-associated proteins in the E. coli cytosol; (C) The workflow of immobilized metal affinity chromatography (IMAC); (D) The workflow of drug pull-down assay
图2 单剂量钌和锇化合物在CT-26肿瘤小鼠组织中的分布。图像为连续H&E染色组织切片图与LA-ICP-MS分析得到的图像
Fig.2 Metal distribution in tissues of mice bearing a CT-26 tumour treated with a single dose of ruthenium and osmium compounds. The images are obtained from consecutive H&E stained tissue sections and LA-ICP-MS analysis
图3 铋剂药物抑制幽门螺旋杆菌的多靶点作用模式示意图
Fig.3 A model for the multi-targeted mode of action of Bi drug in eradicating H. pylori
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