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化学进展 2022, Vol. 34 Issue (11): 2331-2339 DOI: 10.7536/PC220319 前一篇   后一篇

• 综述 •

单细胞水生生物金属纳米颗粒的定量分析

张丹丹1,2, 吴琪1,3, 曲广波1,2,3,*(), 史建波1,2,3, 江桂斌1   

  1. 1 中国科学院生态环境研究中心 环境化学与生态毒理学国家重点实验室 北京 100085
    2 中国科学院大学资源与环境学院 北京 100049
    3 中国科学院大学杭州高等研究院环境学院 杭州 310024
  • 收稿日期:2022-03-22 修回日期:2022-04-21 出版日期:2022-11-24 发布日期:2022-06-25
  • 通讯作者: 曲广波
  • 作者简介:

    曲广波 研究员,博士生导师。2011年博士毕业至今在中国科学院生态环境研究中心工作。2014年1月~2015年3月分别在美国印第安纳大学和迈阿密大学任访问学者和博士后。研究方向为新型污染物的转化分析与毒理。通过基于效应导向的分析技术与成组毒理学分析系统,对区域环境的暴露和效应风险进行评价并筛选效应污染物。在Chem. Rev., Angew. Chem. Int. Ed.,Environ. Sci. & Technol.和ACS Nano等期刊发表SCI论文86篇。2017年获得"国家优秀青年基金"支持。

  • 基金资助:
    国家自然科学基金项目(21976189); 国家自然科学基金项目(22036002)
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Quantitative Analysis of Metal Nanoparticles in Unicellular Aquatic Organisms

Dandan Zhang1,2, Qi Wu1,3, Guangbo Qu1,2,3(), Jianbo Shi1,2,3, Guibin Jiang1   

  1. 1 State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences,Beijing 100085, China
    2 College of Resources and Environment, University of Chinese Academy of Sciences,Beijing 100049, China
    3 School of Environment, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences,Hangzhou 310024, China
  • Received:2022-03-22 Revised:2022-04-21 Online:2022-11-24 Published:2022-06-25
  • Contact: Guangbo Qu
  • Supported by:
    National Natural Science Foundation of China(21976189); National Natural Science Foundation of China(22036002)

人类活动释放的金属纳米颗粒不可避免地进入水环境中。大量研究表明,金属纳米颗粒会对水生生物产生生殖毒性和遗传毒性等,金属纳米颗粒还可能沿着食物链传递,对环境生物和人类健康造成威胁。细胞内金属纳米颗粒定量分析是研究金属纳米颗粒生物效应的重要基础。此外,单细胞之间存在异质性,具有特殊生理特性的细胞个体可能影响细胞群体的命运。而基于细胞群体平均值的定量分析则忽略了细胞个体的异质性,遗漏了对群落具有重要功能的细胞群体信息。因此,在单细胞水平上定量分析水环境中底层营养级的单细胞微生物细胞内金属纳米颗粒,对认识金属纳米颗粒与水生生物的相互作用,评估其进入食物链的潜在风险至关重要。本文梳理了已用于单细胞水生生物体内金属纳米颗粒的单细胞定量分析方法,阐述了它们的工作原理和在相关研究中的应用,总结了各方法的优缺点,期望为今后相关研究的方法选择提供参考,最后展望了该领域未来的研究方向。

关键词： 单细胞水生生物, 金属纳米颗粒, 单细胞分析, 定量分析

Metal nanoparticles released by human activities inevitably enter the aquatic environment. Numerous studies have shown that metal nanoparticles could induce reproductive toxicity and genetic toxicity in aquatic organisms. Furthermore, metal nanoparticles could be transmitted along the food chains which is a potential threat to human health. Quantitative analysis of intracellular metal nanoparticles is important for studying the biological effects of metal nanoparticles. In addition, there is heterogeneity among cells, and the individual cell with special physiological characteristics may influence or even determine the destiny of the cell population. However, most traditional methods for the quantitative determination of intracellular metal nanoparticles were based on the whole cell population whereas the heterogeneity of individual cells was neglected, which may result in the loss of significant information about cell populations that have important functions to the community. Therefore, the quantitative analysis of the intracellular metal nanoparticles in unicellular microorganisms, which locates at the bottom of the trophic level in the aquatic environment, is essential for understanding the interaction between metal nanoparticles and aquatic organisms and evaluating the potential risk of metal nanoparticles entering the food chain. In this review, we discuss the single-cell analysis methods for the quantitative determination of metal nanoparticles in aquatic unicellular organisms. The working principle and application of these methods and their pros and cons are summarized. This paper aims to provide a theoretical basis of the method selection for relevant studies in the future. Finally, we prospect the future research directions in this field according to the current research status.

Contents

1 Introduction

2 Quantitative methods based on fluorescence

2.1 Confocal laser scanning microscopy

2.2 Flow cytometry

3 Quantitative methods based on non-fluorescent microscopy

3.1 Scanning transmission electron microscopy

3.2 Hyperspectral imaging with enhanced darkfield microscopy

4 Quantitative methods based on mass spectrometry

4.1 Single cell inductively coupled plasma mass spectrometry

4.2 Mass cytometry

5 Conclusion and perspective

Key words: unicellular aquatic organisms, metal nanoparticles, single-cell analysis, quantitative analysis
()
表1 本综述中提到的单细胞水生生物体内金属纳米颗粒的定量分析方法的应用概述
Table 1 Overview of the application of methods for quantitative analysis of metal nanoparticles in unicellular aquatic organisms mentioned in this review
Method Metal nanoparticles Organism Highlights ref
CLSM CdSe/ZnS QDs T. thermophila Observation of intracellular location of CdSe/ZnS QDs 34
FCM CdTe QDs O. danica Uptake of CdTe QDs in O. danica via micropinocytosis 37
CdSe/ZnS QDs T. thermophila Lower detection sensitivity of FCM compared with CyTOF 34
nTiO2 P. caudatum Increased internalization of nTiO2 in P. caudatum when E. coli coexists 41
STEM nTiO2 T. thermophila Trophic transfer of nTiO2 from P. aeruginosa to T. thermophila via a food web 47
CdSe/ZnS QDs P. aeruginosa Uptake of CdSe QDs in P. aeruginosa 48
HIS-M AgNPs, AuNPs, nCuO,
nTiO2, CdSe/ZnS QDs		 T. thermophila Semi-quantitative analysis of metal NPs in T. thermophila 55
SC-ICP-MS TeNPs S. aureus and
E. coli		 Heterogeneous accumulation of TeNPs in S. aureus and E. coli 65
AuNPs C. ovate Quantitative analysis of AuNPs in single-cell algae by SC-ICP-MS 66
AuNPs P. subcapitata Particle number-based trophic transfer of gold nanomaterials from P. subcapitata to daphnids and fish in an aquatic food chain 16
AgNPs C.vulgaris Dual mass mode of quadrupole-based SC-ICP-MS for quantitative analysis of two metal elements (Mg and Ag) in C.vulgaris 68
CyTOF AuNPs T. thermophila Heterogeneous internalization of AuNPs by T. thermophila at ultra-trace exposure concentration 34
表2 本综述中提到的单细胞定量分析方法之间优点和缺点的概述
Table 2 Overview of the advantage and disadvantage among single-cell quantitative analysis methods mentioned in this review
Method Advantages Disadvantages
CLSM Intracellular location, enabling living organisms Label needed, phototoxicity, light bleaching, fluorescence quenching
FCM High-throughput of samples, multiple parameters, non-destructive Unable to locate NPs within cells, fluorescence quenching, spectral overlap, uncoupling of fluorescent dyes
STEM High resolution (one particle), no need for labeling, no quenching, and no bleaching Requirement for complex sample preparation
HIS-M High resolution (one particle), no need for labeling, no quenching, bleaching Low sample throughput, long analysis time
SC-ICP-MS High throughput for sampling, no need for labeling, low detection limit (ag/cell) Sample destruction, lower transmission efficiency, unable to locate NPs within cells, aggregates of two or more cells
CyTOF High throughput (500 events/s) for sampling, no need for labeling, high detection sensitivity (ag/cell), multiple parameters, multiple element analysis, no need for labeling Sample destruction, lower transmission efficiency
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