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化学进展 2019, Vol. 31 Issue (11): 1615-1622 DOI: 10.7536/PC190808 前一篇   

• •

外场强化环境响应固相萃取技术

杨良嵘1,3, 邢慧芳1,3, 屈虹男1,3, 于杰淼1,3, 刘会洲1,2,3,**()   

  1. 1. 中国科学院过程工程研究所 北京 100190
    2. 中国科学院文献情报中心 北京 100190
    3. 中国科学院大学化学工程学院 北京 100049
  • 收稿日期:2019-08-08 出版日期:2019-11-15 发布日期:2019-11-06
  • 通讯作者: 刘会洲
  • 基金资助:
    国家自然科学基金项目(21922814); 国家自然科学基金项目(21676273); 国家自然科学基金项目(U1507203); 国家自然科学基金项目(31961133019); 国家自然科学基金项目(21921005); 中科院青年创新促进人才项目(2016043); 北京市自然科学基金项目(2194086)
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External Field Enhanced Environmental Responsive Solid Extraction Technology

Liangrong Yang1,3, Huifang Xing1,3, Hongnan Qu1,3, Jiemiao Yu1,3, Huizhou Liu1,2,3,**()   

  1. 1. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
    2. National Science Library, Chinese Academy of Sciences, Beijing 100190, China
    3. College of Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2019-08-08 Online:2019-11-15 Published:2019-11-06
  • Contact: Huizhou Liu
  • About author:
    ** E-mail:
  • Supported by:
    National Natural Science Foundation of China(21922814); National Natural Science Foundation of China(21676273); National Natural Science Foundation of China(U1507203); National Natural Science Foundation of China(31961133019); National Natural Science Foundation of China(21921005); Chinese Academy of Sciences Youth Innovation Promotion Talent Project(2016043); Beijing Natural Science Foundation Project(2194086)

低浓度大体量复杂溶液中目标物的高效、精准、可控分离是当今化工分离科学领域的世界前沿课题。固相萃取技术目前用于低浓度复杂体系工业分离面临两方面挑战:一方面是高选择性精准捕获与温和绿色解吸难以兼具。另一方面是固相萃取技术缺少规模连续化分离的高效分离工艺设备。本文综述了为解决当前固相萃取技术存在的问题,实现低浓度复杂体系的高效精准和可控分离,新型环境响应固相萃取技术,磁场响应固相萃取技术以及电场、超声场辅助固相萃取技术取得的研究进展。最后,关于该应用领域固相萃取技术的研究发展方向进行了展望。本文对外场强化环境响应固相萃取技术的关键科学问题,包括环境响应问题、可控分离问题和过程放大问题进行了深入探讨,并对相关领域的发展提出了措施与建议。

关键词： 外场强化, 环境响应, 固相萃取, 磁分离, 萃取分离

Efficient, precise and controllable separation of target compounds in low concentration and large volume complex solutions is a world frontier in the field of chemical separation science. Solid phase extraction technology currently faces two challenges in industrial separation of low-concentration complex systems. On the one hand, it is difficult to give consideration to both of the high selective precision capture and the mild green desorption. On the other hand, solid phase extraction technology lacks efficient separation process and equipment for large scaled continuous separation. This review summarizes the research progress of new environmental responsive solid phase extraction technology, magnetic field responsive solid phase extraction technology, electric field- and ultrasound field- assisted solid phase extraction technology in order to solve the problems of current solid phase extraction technology, and achieve the efficient and precise separation for low concentration complex systems. Finally, the research and development direction of solid phase extraction technology in this application field is prospected. In this review, the key scientific issues of external field enhanced environmental responsive solid phase extraction technology, including environmental responsive problems, controllable separation problems and process amplification problems, are further discussed. Besides, measures and suggestions are proposed for the development of related fields.

Key words: external field enhancement, environmental responsive, solid phase extraction, magnetic separation, extraction separation
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图1 固相萃取示意图
Fig. 1 Schematic diagram of solid phase extraction
图2 固相萃取应用于低浓度复杂体系工业分离挑战
Fig. 2 The challenges of solid phase extraction applied in complex industrial separation systems with low concentrations
图3 新型环境响应固相萃取技术及磁场响应固相萃取技术示意图
Fig. 3 Schematic diagram of novel environmental responsive solid phase extraction technology and magnetic field responsive solid phase extraction technology
图4 二维红外同步相关光谱(a)和异步相关光谱(b)[12]
Fig. 4 Two-dimensional infrared synchronization correlation spectrum(a) and asynchronous correlation spectrum(b)[12]
图5 温度/pH响应的磁性纳米颗粒界面调控聚集解聚行为[14]
Fig. 5 Temperature /pH responsive magnetic nanoparticle and interface regulated aggregation and depolymerization behavior[14]
图6 温度响应适配体固相萃取吸附剂界面调控凝血酶分离
Fig. 6 Temperature responsive aptamer solid phase extraction adsorbent and interface regulated separation of thrombin
图7 光响应适配体固相萃取吸附剂界面调控凝血酶可控分离
Fig. 7 Light responsive aptamer solid phase extraction adsorbent and interface regulated separation of thrombin
图8 新型固相萃取技术与传统方法对比
Fig. 8 The comparation between novel solid phase extraction technology and the traditional method
图9 磁响应固相萃取技术
Fig. 9 Magnetic responsive solid phase extraction technology
图10 吸附速率慢原因分析
Fig. 10 Analysis of reasons for slow adsorption rate
表1 国内外同行比较
Table 1 Comparison of domestic and foreign counterparts
Adsorbent qm
(mg/g)		 E. T.
(min)		 pH range Ref
γ-Fe2O3@δ-FeOOH 25.8 — 2.5 25
Fe3O4@PAA-DETA 11.24 — 5.0 26
NiFe2O4 30 — 5.0 27
Fe3O4-PEI-MMT 8.8 — 5.0 28
Fe3O4-Cyanex-301 30.8 120 2.0 29
ZnFe2O4-Ce3+ 57.24 4320 2.0 30
Poly(MMA-DVB-GMA)-EDA 61.35 60 2.0~3.0 31
Fe3O4@polypyrrole 169.49 180 1.0~6.0 32
Poly(GMA-EGDMA)-PEI 137.7 120 1.0~6.0 33
Poly(MA-DVB)-EDA ~36 60 3.0 34
Magnetic chitosan beads 69.4 > 60 3.0~5.0 19b
Magnetic chitosan-EDA 51.813 10 2.0 35
Magnetic chitosan-CAGS 58.48 110 2.0 19c
Chitosan/ montmorillonite 35.71 100 1.0~3.0 36
Magnetic chitosan nanoparticles 55.80 150 2.0~3.0 37
Cyclodextrin-chitosan/GO 67.66 — 1.0~3.0 38
MnFe2O4/Chitosan 15.4 360 6.0 39
MnFe2O4-Chitosan 51.79 480 5.0 39
Fe3O4-Fungus@alginate-PAA 6.97 720 1.0 40
Fe3O4@PVA-PEI 88.4 8 1.0~3.0 41
Fe3O4@PGMA-PEI 492.6 10 1.0~7.0 42
Fe3O4@Pst-MIMCl 104.0 30 1.0~7.0 43
Fe3O4@SiO2@CE-EDA 171.5 10 1.0~6.0 44
Fe3O4@SiO2@CTS-PEI 236.4 60~120 1.0~6.0 45
Fe3O4@SiO2@CTS-GTMAC 233.1 40~120 1.0~7.0 43
图11 磁分离装置连续放大难点
Fig. 11 Difficulties in continuous amplification of magnetic separation devices
图12 气助超顺磁性连续化萃取分离新工艺和设备
Fig. 12 Novel gas-assisted superparamagnetic continuous extraction technology and equipment
图13 气助磁分离新工艺用于蛋白连续分离纯化[46]
Fig. 13 Gas-assisted magnetic separation used for continuous separation and purification of protein[46]
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摘要

外场强化环境响应固相萃取技术