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化学进展 2017, Vol. 29 Issue (2/3): 300-317 DOI: 10.7536/PC160929 前一篇   后一篇

• 综述 •

量子点荧光传感器的设计及应用

胡先运1,2*, 郭庆生1, 刘玉乾1, 孙清江1*, 孟铁宏2, 张汝国2   

  1. 1. 东南大学生物科学与医学工程学院 生物电子学国家重点实验室 南京 210096;
    2. 黔南民族医学高等专科学校 都匀 558000
  • 收稿日期:2016-09-27 修回日期:2016-12-21 出版日期:2017-02-15 发布日期:2017-02-27
  • 通讯作者: 胡先运, 孙清江 E-mail:huxianyun2004@163.com;sunqj@seu.edu.cn
  • 基金资助:
    国家自然科学基金项目（No.21545006，21375015）、贵州省自然科学基金项目（No.2015GZ48861，2016GZ13752）、中央高校基本科研业务费专项资金项目（No.KYLX0187）、黔南科技计划项目（黔南科合工字[2016]12号）以及黔南民族医专科研基金项目（No.QNYZ201601）资助
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Design Strategies and Applications of Quantum Dots Fluorescent Sensing

Xianyun Hu1,2*, Qingsheng Guo1, Yuqian Liu1, Qingjiang Sun1*, Tiehong Meng2, Ruguo Zhang2   

  1. 1. State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;
    2. Qiannan Medical College for Nationalities, Duyun 558000, China
  • Received:2016-09-27 Revised:2016-12-21 Online:2017-02-15 Published:2017-02-27
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (No. 21545006,21375015), the National Science Foundation of Guizhou Province (No. 2015GZ48861, 2016GZ13752), the Fundamental Research Funds for the Central Universities (No. KYLX0187) and the Scientific Research fund of Qiannan Medical College for Nationalities (No.QNYZ201601).
量子点（quantum dots，QDs）是一种新型的纳米荧光材料，具有优良的光电性质，已广泛应用于荧光传感及可视化检测，可实现对靶标分子高灵敏、高特异性分析。本文主要论述了量子点的表面化学修饰，以及量子点传感的作用原理，如荧光共振能量转移、电荷转移、直接荧光传感、生物发光共振能量转移、化学发光共振能量转移以及电化学发光，利用这些原理设计出不同的荧光传感器，并应用于不同分子或离子的可视化检测。同时对量子点的荧光传感存在的问题及挑战进行了总结，并提出量子点荧光传感将向生物相容性好、细胞或生物体内实时可视化检测、复杂体系中进行多靶标同时检测以及量子点的逻辑运算等方向发展。
关键词： 量子点, 荧光传感, 探针, 可视化检测
Quantum dots (QDs), as novel fluorescent nanomaterials, have been widely used in fluorescent sensing and visual detection with high sensitivity and specificity analysis of target, owing to their excellent optical and electrical properties. The review addresses the surface functionalization of QDs that able to the use of sensing, and discusses different sensing mechanism including fluorescence resonance energy transfer (FRET), electron transfer (CT), direct fluorescent sensing, bioluminescence resonance energy transfer (BRET), chemiluminescence resonance energy transfer (CRET) and electrochemiluminescence (ECL), being applied in the different sensing systems. The challenges and existing problems of QDs fluorescent sensing application are summarized. Meanwhile fluorescent sensing of QDs will develop in the field of the good biocompatibility, real time visualization of sensing in cells or in vivo, multiplexed detection in complex systems and the function of sensing with logic-gate operations in the future.

Contents
1 Introduction
2 Photo-properties of quantum dots
3 Surface chemistry and conjugates of quantum dots
4 Mechanisms and design strategies of quantum dots fluorescence sensing
4.1 Fluorescence resonance energy transfer
4.2 Electron transfer
4.3 Direct fluorescent sensing
4.4 Bioluminescence resonance energy transfer
4.5 Chemiluminescence resonance energy transfer
4.6 Electrochemiluminescence
5 Conclusion

Key words: quantum dots, fluorescent sensing, probe, visualization detection

中图分类号: 

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摘要

量子点荧光传感器的设计及应用