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化学进展 2016, Vol. 28 Issue (1): 91-102 DOI: 10.7536/PC150633 前一篇   后一篇

• 综述与评论 •

铁蛋白表面修饰及其应用

杨彩云1,2,3, 曹长乾1,2, 蔡垚1,2,3, 张同伟1,2, 潘永信1,2*   

  1. 1. 中法生物矿化与纳米结构联合实验室 中国科学院地质与地球物理研究所 北京 100029;
    2. 中国科学院地球与行星物理重点实验室 中国科学院地质与地球物理研究所 北京 100029;
    3. 中国科学院大学 北京 100049
  • 收稿日期:2015-06-01 修回日期:2015-09-01 出版日期:2016-01-15 发布日期:2015-12-21
  • 通讯作者: 潘永信 E-mail:yxpan@mail.iggcas.ac.cn
  • 基金资助:
    中科院/国家外国专家局创新团队国际合作伙伴计划项目(No.KZCX2-YW-T10),国家自然科学基金项目(No.41330104,41204053)和国家重大科研装备研制项目(No.ZDYZ2012-1-01-02)资助
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The Surface Modification of Ferritin and Its Applications

Yang Caiyun1,2,3, Cao Changqian1,2, Cai Yao1,2,3, Zhang Tongwei1,2, Pan Yongxin1,2*   

  1. 1. France-China Bio-Mineralization and Nano-Structures Laboratory, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100029, China;
    2. Key Laboratory of Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100029, China;
    3. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2015-06-01 Revised:2015-09-01 Online:2016-01-15 Published:2015-12-21
  • Supported by:
    The work was supported by the CAS/SAFEA International Partnership Program for Creative Research Teams (No. KZCX2-YW-T10), the National Natural Science Foundation of China (No. 41330104, 41204053), and the R&D of Key Instruments and Technologies for Deep Resources Prospecting (No. ZDYZ2012-1-01-02).
铁蛋白是一种广泛存在的储铁蛋白,具有纳米尺寸的水合氧化铁内核和笼形结构的蛋白质外壳。通过对铁蛋白壳的修饰或核的改造已成功构建出多功能肿瘤诊断和药物输送系统。近年来对铁蛋白的修饰研究主要集中在:(1)通过对铁蛋白内表面的修饰使铁蛋白壳内包裹上特定药物或者促进纳米材料的合成;(2)通过对铁蛋白外表面的修饰与PEG或抗体连接以扩展新的功能;(3)通过铁蛋白外表面或亚基间接触面的修饰控制铁蛋白的自组装。目前从铁蛋白修饰角度来阐明铁蛋白应用的研究较少。本文综述了近年来铁蛋白表面修饰的研究进展,介绍了铁蛋白表面化学修饰和生物修饰的方法,并进一步阐述了经修饰后铁蛋白纳米材料在生物医学、诊断学、纳米电子学等领域的应用。最后探讨了目前铁蛋白表面修饰研究方向及需解决的问题,提出了将铁蛋白生物修饰和化学修饰两种方法相结合是未来发展方向之一。本工作旨在为铁蛋白的进一步开发利用提供一些可能的思路。
关键词： 铁蛋白, 纳米材料, 表面修饰, 应用
Ferritin is a spherical protein composed of 24 subunits with an outer diameter of 12 nm and inner cavity diameter of 8 nm. The inner cavity can accommodate up to 4500 iron atoms as an iron mineral core (ferrihydrite in original). This special architecture of ferritin makes it an ideal nanoscale biotemplate. With the modification of the protein shell and the transformation of the inner mineral core, researchers have developed series of multifunctional cancer diagnostic agents and drug delivery systems against tumors. Recent studies of ferritin modification have focused on: (1) interior modification to make the nucleation of ferritin more efficient;(2) exterior modification by connecting PEG or antibody on the protein shell to develop ferritin new functions; (3) modification of interface between subunits to control the self-assemble of ferritin. This review summarizes the recent advances in the surface modifications of ferritin. We firstly review the two different modified methods, the chemical modification and the biological modification, then, discuss the diverse applications with modified ferritin in biomedicine, diagnostics and nano electronics, and the existing problems in the surface modifications of ferritin. We propose that a way to develop novel functionalized ferritin-based nano-materials is to combine the chemical and biological means.

Contents
1 Introduction
2 Structure of ferritin
3 The surface modification of ferritin and its applications
3.1 The surface modification of ferritin with chemical methods
3.2 Applications of chemically modified ferritin
3.3 The surface modification of ferritin with biological methods and its applications
4 Conclusion and outlook

Key words: ferritin, nano-materials, surface modification, application

中图分类号: 

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摘要

铁蛋白表面修饰及其应用