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化学进展 2015, Vol. 27 Issue (9): 1251-1259 DOI: 10.7536/PC150212 前一篇   后一篇

所属专题： 酶化学

• 综述与评论 •

碳纳米管固定化酶

万晓梅, 张川, 余定华, 黄和, 胡燚*   

  1. 南京工业大学生物与制药工程学院 材料化学工程国家重点实验室 南京 210009
  • 收稿日期:2015-02-01 修回日期:2015-04-01 出版日期:2015-09-15 发布日期:2015-06-24
  • 通讯作者: 胡燚 E-mail:huyi@njtech.edu.cn
  • 基金资助:
    国家高技术研究发展计划(No.2011AA02A209)和国家杰出青年科学基金项目(No. 21225626)资助
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Enzyme Immobilized on Carbon Nanotubes

Wan Xiaomei, Zhang Chuan, Yu Dinghua, Huang He, Hu Yi*   

  1. College of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 210009, China
  • Received:2015-02-01 Revised:2015-04-01 Online:2015-09-15 Published:2015-06-24
  • Supported by:
    The work was supported by the National High Technology Research and Development of China (No.2011AA02A209) and the National Science Foundation for Distinguished Young Scholars of China (No. 21225626).
在固定化酶技术中,载体材料的选择至关重要,碳纳米管作为一种新型高效的酶固定化载体,具有较大的比表面积、有序的纳米孔道结构、良好的力学/电学/热学性能、突出的化学稳定性、生物相容性和可控的表面官能化修饰等优良特性,应用日益广泛。本文重点介绍了水解酶、氧化还原酶等具有重要工业应用价值的酶在碳纳米管上的固定化研究现状,探讨了载体的表面修饰和固定化方式对固定化酶的酶学性质的影响,并对碳纳米管固定化酶的发展前景进行了展望。
关键词： 碳纳米管, 固定化, 酶, 水解酶, 氧化还原酶
In the technology of enzyme immobilization, the choice of carrier material is very important for the catalytic performance of enzyme. As a new type of high efficient enzyme immobilization carrier with larger specific surface area, ordered nano pore structure, good mechanical/electrical/thermal performance, outstanding chemical stability, biocompatibility and controllable surface functional modifications, carbon nanotubes are used more and more extensively. The recent research progress of enzyme immobilization on carbon nanotubes is reviewed in this paper, focusing on the immobilization of hydrolases and oxido-reductases, which have important industrial application value. The influence of surface modification of carrier and immobilizationmethods on the catalytic properties of immobilized enzymes is introduced. The outlook of potential applications of enzyme immobilization on carbon nanotubes is also prospected.

Contents
1 Introduction
2 Research of enzyme immobilization on carbon nanotubes
2.1 Hydrolase immobilization on carbon nanotubes
2.2 Oxido-reductase immobilization on carbon nanotubes
2.3 Other kinds of enzymes immobilization on carbon nanotubes
2.4 The application of immobilized enzymes in the biosensors
3 Conclusions

Key words: carbon nanotubes, immobilization, enzyme, hydrolase, oxido-reductase

中图分类号: 

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摘要

碳纳米管固定化酶