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化学进展 2014, Vol. 26 Issue (06): 1050-1064 DOI: 10.7536/PC131115 前一篇   后一篇

• 综述与评论 •

基于微流控技术的单细胞生物物理特性表征

唐文来, 项楠, 黄笛, 张鑫杰, 顾兴中, 倪中华*   

  1. 东南大学 江苏省微纳生物医疗器械设计与制造重点实验室 南京 211189
  • 收稿日期:2013-11-01 修回日期:2014-02-01 出版日期:2014-06-15 发布日期:2014-03-31
  • 通讯作者: 倪中华 E-mail:nzh2003@seu.edu.cn
  • 基金资助:

    国家重点基础研究发展计划（973）项目（No.2011CB707601）、国家自然科学基金项目（No.91023024，51375089）、高等学校博士学科点专项科研基金项目（No.20110092110003）和江苏省自然科学基金项目（No.BK2011336）资助

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Microfluidics-Based Single-Cell Biophysical Characterization

Tang Wenlai, Xiang Nan, Huang Di, Zhang Xinjie, Gu Xingzhong, Ni Zhonghua*   

  1. Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing 211189, China
  • Received:2013-11-01 Revised:2014-02-01 Online:2014-06-15 Published:2014-03-31
  • Supported by:

    The work was supported by the National Basic Research Program of China (No.2011CB707601), the National Natural Science Foundation of China (No.91023024, 51375089), the Specialized Research Fund for the Doctoral Program of Higher Education (No.20110092110003) and the Natural Science Foundation of Jiangsu Province (No.BK2011336)

单细胞水平的生物物理特性表征，可有效阐明细胞的功能和状态，揭示细胞的单体差异性，对于细胞的分化和病理研究，以及疾病的早期临床诊断和治疗具有非常重要的意义。由于具有与细胞尺度相匹配的微米级腔道，微流控芯片比传统生化方法更适合单细胞样本的微环境精确控制、高通量定向操纵及多参数非特异性检测，已成为单细胞表征与分析的一项重要技术平台。本文总结了基于微流控技术的单细胞生物物理特性表征方法及其应用的最新进展，着重分析微流控芯片在常规方法难以达成的单细胞和高通量研究中的独特优势，最后探讨了微流控单细胞生物物理特性检测芯片在临床应用中面临的挑战和未来的发展动向，并提出一种新型的单细胞多参数同时表征的微流控分析器件。

关键词： 单细胞, 生物物理特性, 微流控, 高通量, 多参数

Single-cell biophysical characterization has been widely employed for demonstrating the physiological activity and status of individual cells, or revealing the heterogeneity among various populations. It also plays an important role in the studies on cellular differentiation and pathology, as well as early clinical diagnosis and treatment. Compared to conventional biochemical schemes, the feature of scale compatibility between cell and microchannel makes microfluidics more suitable for precise microenvironment control, high-throughput manipulation, and multi-parameter label-free detection of individual cells. Therefore, microfluidics has become an important platform for single-cell characterization and analysis. This review covers the recent advances in microfluidics for characterizing the single-cell biophysical properties, and then focuses on the discussion of specific advantages, such as single-cell resolution level and high-throughput feature, offered by microfluidics. Finally, the challenges and future directions concerning the application of this scheme in clinical practices are also discussed, and a novel single-cell microdevice for multi-parameter characterization is proposed.

Contents
1 Introduction
2 Density(Mass) characterization
3 Electrical characterization
3.1 Microfluidic Coulter counter
3.2 Microfluidic impedance cytometer
4 Mechanical characterization
4.1 Optical stretcher
4.2 Electroporation-induced deformation
4.3 Structure-induced deformation
4.4 Fluid-induced deformation
5 Multi-parameter biophysical characterization
6 Conclusion and outlook

Key words: single-cell, biophysical properties, microfluidics, high-throughput, multi-parameter

中图分类号: 

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