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化学进展 2014, Vol. 26 Issue (05): 846-855 DOI: 10.7536/PC131035 前一篇   后一篇

• 综述与评论 •

金表面巯基化DNA单层性能的影响因素研究

李志果*, 张玲玲   

  1. 湛江师范学院化学科学与技术学院 物理化学研究所 湛江 524048
  • 收稿日期:2013-12-01 修回日期:2013-12-01 出版日期:2014-05-15 发布日期:2014-03-13
  • 通讯作者: 李志果，e-mail：zgli_zjnu@163.com E-mail:zgli_zjnu@163.com
  • 基金资助:

    国家自然科学基金项目（No. 21205105）；广东省自然科学基金项目（No. S2012040007348）和湛江师范学院博士专项基金项目（No. ZL1103）资助

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Influence Factors on the Performance of DNA Self-Assembled Monolayers on Gold

Li Zhiguo*, Zhang Lingling   

  1. School of Chemistry Science and Technology, Institute of Physical Chemistry, Zhanjiang Normal University, Zhanjiang 524048, China
  • Received:2013-12-01 Revised:2013-12-01 Online:2014-05-15 Published:2014-03-13
  • Supported by:

    The work was supported by the National Natural Science Foundation of China (No. 21205105), Guangdong Natural Science Foundation (No. S2012040007348) and Doctoral Special Foundation of Zhanjiang Normal University (No. ZL1103)

巯基化DNA在金表面上吸附构建的自组装单层（self-assembled monolayers，SAMs）为DNA研究的理想异相体系，在DNA内电子传递的机制探讨、高灵敏DNA传感器设计以及DNA碱基错配点识别等方面有着广泛的应用。对于不同的研究对象应构建性能适合的DNA单层，以满足研究需要。为了可控构建DNA单层，应理解不同因素对金表面上DNA单层性能的影响。本文从金表面状况、DNA特性以及周围环境三个方面对金表面上巯基化DNA单层性能的影响因素研究进行了系统综述。

关键词： 金, 巯基化DNA, 自组装单层, 影响因素

Thiol-modified DNA self-assembled monolayers (SAMs) on gold is the ideal heterogeneous system for studying DNA charge transfer through DNA duplex, designing DNA sensor with high sensitivity and identifying single base mismatch etc. The performance of DNA SAMs on gold is related to three aspects: gold surface conditions, DNA characteristics and surroundings. Gold surface conditions mainly include substrate configuration by different surface pretreatment, gold shapes, substrate potential and substrate temperature; DNA characteristics include the difference of double-stranded and single-stranded DNA, DNA base types, types for thiols modification and factors related to DNA electron transfer; Surroundings mainly include ionic strength or types of cations, ambient medium, temperature in solution and types of mixed thiols. These factors influence surface density and conformation of DNA SAMs on gold, which determine its charge transfer or hybridization performance. In order to controllably construct DNA SAMs with the optimal performance for satisfying different researches about DNA, it is very necessary to understand the effect of different factors on the performance of DNA SAMs on gold. In this article we review the research progress of influence factors on the performance of DNA SAMs on gold from three aspects including gold surface conditions, DNA characteristics and surroundings.

Contents
1 Introduction
2 Gold surface conditions
2.1 Surface pretreatment
2.2 Gold substrate shapes
2.3 Gold substrate potential
2.4 Gold substrate temperature
3 DNA characteristics
3.1 Double-stranded and single-stranded DNA
3.2 DNA base types
3.3 Types for thiols modification
3.4 Factors related to DNA electron transfer
4 Surroundings
4.1 Ionic strength and types of cations
4.2 Ambient medium
4.3 Temperature in solution
4.4 Types of mixed thiols
5 outlook

Key words: gold, thiol-modified DNA, self-assembled monolayers, influence factor

中图分类号: 

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