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化学进展 DOI: 10.7536/PC121236 前一篇   后一篇

• 综述与评论 •

基于硝基苯并呋咱的荧光探针

古振远, 徐勤超, 邢国文*   

  1. 北京师范大学化学学院 北京 100875
  • 收稿日期:2012-12-01 修回日期:2013-01-01 出版日期:2013-08-25 发布日期:2013-06-13
  • 通讯作者: 邢国文 E-mail:gwxing@bnu.edu.cn
  • 基金资助:

    国家自然科学基金项目(No. 21272027)资助

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导出 被引次数 ( 6 | 2 )

Nitrobenzofurazan-Based Fluorescent Probes

Gu Zhenyuan, Xu Qinchao, Xing Guowen*   

  1. College of Chemistry, Beijing Normal University, Beijing 100875, China
  • Received:2012-12-01 Revised:2013-01-01 Online:2013-08-25 Published:2013-06-13

硝基苯并呋咱(NBD)作为一种通常从4-氯-7-硝基-苯并呋咱衍生化的荧光团,在荧光分析中具有简便、高效、灵敏度高以及检测限低等显著优点,已广泛应用于化学、生物、医药和环境等诸多研究领域。基于NBD的荧光探针是检测和识别重要的无机/有机小分子化合物、酶和蛋白质的研究热点之一。本文综述了近年来NBD作为荧光团在重金属离子(Zn2+, Cu2+ 和 Hg2+)、活性氧、DNA、细胞膜、小分子化合物(硫酚、糖类、金刚烷/胆酸、氟甲沙明/埃博霉素、双酚A、多胺、TNT和PPi)、酶和蛋白质等检测领域的新进展。在不久的将来,设计合成结构更加精巧的NBD探针分子仍将是荧光检测和生物成像领域的主要发展趋势之一。这些新颖的NBD探针分子的应用将为全面深入探索与理解复杂的生命过程提供强有力的研究工具。

关键词： 硝基苯并呋咱, 荧光探针, 金属离子, 酶

Nitrobenzofurazan (NBD), a fluorophore usually derivated from 4-chloro-7-nitrobenzofurazan, has been extensively applied to a wide area of chemistry, biology, medicine and environmental science due to its simplicity, effectiveness, high sensitivity and low detection limit in the fluorometric analysis. The fluorescent probes based on NBD have become an important research focus in the sensing and recognition of important inorganic/organic molecules, protein and enzymes. This article reviews the recent progress of NBD-involved detection on heavy metal cations (Zn2+, Cu2+ and Hg2+), reactive oxygen species, DNA, cell membranes, small molecular compounds (thiophenols, sugar compounds, adamantane and bile acid derivatives, fluvoxamine and epothilone, bisphenol A, polyamine, TNT, PPi), proteins, enzymes, etc. In the future, the design and synthesis of more exquisite and complex probes based on NBD is still a flourishing research area for fluorescent detection and biochemical imaging, and will provide a particularly applicable tool to study the intricate microcosmic process of life. Contents
1 Introduction
2 Fluorescence detection of heavy metal ions
2.1 Fluorescent probes for Hg2+
2.2 Fluorescent probes for Zn2+
2.3 Fluorescent probes for Cu2+
3 Fluorescence detection of reactive oxygen species
4 Fluorescent probe for DNA
5 Fluorescence detection of membranes
6 Fluorescence detection of small molecules
6.1 Fluorescence detection of thiophenols
6.2 Fluorescence detection of sugar compounds
6.3 Fluorescence detection of adamantane and bile acid derivatives
6.4 Fluorescence detection of fluvoxamine and epothilone
6.5 Fluorescence detection of bisphenol A
6.6 Fluorescence detection of polyamine
6.7 Fluorescence detection of TNT
6.8 Fluorescence detection of PPi
7 Fluorescence detection of proteins and enzymes
8 Fluorescence detection related to the polarity of micro-environment
9 Conclusion and outlook

Key words: nitrobenzofurazan, fluorescent probe, metal cation, enzyme

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摘要

基于硝基苯并呋咱的荧光探针