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化学进展 2013, Vol. 25 Issue (07): 1198-1207 DOI: 10.7536/PC121161 前一篇   后一篇

所属专题： 酶化学

• 综述与评论 •

固定化酶微反应器的制备及应用

申刚义1*, 于婉婷1, 刘美蓉2, 崔勋1,3*   

  1. 1. 中央民族大学中国少数民族传统医学研究院 北京 100081;
    2. 中国科学院化学研究所 北京 100190;
    3. 韩国又石大学汉药科 全州 565-701 韩国
  • 收稿日期:2012-11-01 修回日期:2013-03-01 出版日期:2013-07-25 发布日期:2013-04-16
  • 通讯作者: 申刚义, 崔勋 E-mail:sgy@iccas.ac.cn; cuixunws@hotmail.com
  • 基金资助:

    国家自然科学基金项目(No.81001595)、中央高校基本科研业务费专项资金(中央民族大学自主科研项目No.0910KYQN67)和校级课题项目(No.60344302)资助

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Preparation and Application of Immobilized Enzyme Micro-Reactor

Shen Gangyi1*, Yu Wanting1, Liu Meirong2, Cui Xun1,3*   

  1. 1. Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China;
    2. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
    3. Department of Oriental Pharmacy, Woosuk University,Wanju-gun,Jeonbuk 565-701,South Korea
  • Received:2012-11-01 Revised:2013-03-01 Online:2013-07-25 Published:2013-04-16

固定化酶微反应器是将生物分子固定技术与生化微反应相结合制备的一种固定化催化装置。这种微型化的反应系统由于兼具固定化酶的特异性催化、可重复利用及微分析的低消耗、易分离等优点,在生命科学如蛋白质组学、酶抑制剂的筛选、生物催化等领域具有非常重要的作用。固定化酶微反应器的性能与其制作方法关系密切。本文着重从酶与固定化载体结合方式的角度,对近年来固定化酶微反应器的各种制备方法和应用进行了较为详细的评述。重点讨论了各种方法的优缺点和最新的发展情况,并对其发展前景进行了展望。

关键词： 固定化酶微反应器, 制备, 蛋白质组学, 酶抑制剂的筛选, 生物催化

As one kind of new biochemical reaction device, immobilized enzyme micro-reactor is the combination of biomolecule immobilizing technique and modern micro-reaction method. In view of its advantages in efficiency, economy and addressable recognition specially, micro-reactor plays a significant role in the research of life science, such as proteomics, screening of enzyme inhibitors, biocatalysis and so on. With the development of immobilizing materials and fabrication methods, the performance of enzyme micro-reactor has been improved greatly, and enzyme micro-reactor has been applied to many research fields. This article focuses on the preparation methods and the applications of immobilized enzyme micro-reactor for the past few years. The advantages and shortcomings of the current state-of-the-art preparation methods are particularly discussed. In addition, the prospects of its future study are outlined. Contents
1 Introduction
2 Preparation of immobilized enzyme micro-reactor
2.1 Convalent bonding
2.2 Physical adsorption
2.3 Encapsulation
2.4 Metal-ion chelated adsorption
2.5 Biological binding
3 Applications
4 Outlook

Key words: immobilized enzyme micro-reactor, preparation, proteomics, screening of enzyme inhibitors, biocatalysis

中图分类号: 

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摘要

固定化酶微反应器的制备及应用