English
往期目录
新闻公告
More
化学进展 2022, Vol. 34 Issue (3): 568-579 DOI: 10.7536/PC210613 前一篇   后一篇

• 综述 •

肽自组装水凝胶的制备及在生物医学中的应用

李红1, 史晓丹1, 李洁龄2,*()   

  1. 1 西安石油大学化学化工学院 西安 710065
    2 中国科学院过程工程研究所生化工程国家重点实验室 北京 100190
  • 收稿日期:2021-06-16 修回日期:2021-08-23 出版日期:2021-09-27 发布日期:2021-09-27
  • 通讯作者: 李洁龄
  • 基金资助:
    国家自然科学基金项目(22072155); 国家自然科学基金项目(21703169); 陕西省创新人才推进计划-青年科技新星项目(2021KJXX-39)
Richhtml ( 63 ) 下载PDF ( 836 ) 引用
导出

Self-Assembled Peptide Hydrogel for Biomedical Applications

Hong Li1, Xiaodan Shi1, Jieling Li2()   

  1. 1 College of Chemistry and Chemical Engineering, Xi'an Shiyou University,Xi'an 710065, China
    2 State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences,Beijing 100190, China
  • Received:2021-06-16 Revised:2021-08-23 Online:2021-09-27 Published:2021-09-27
  • Contact: Jieling Li
  • Supported by:
    National Natural Science Foundation of China(22072155); National Natural Science Foundation of China(21703169); Scientific Research Plan of Shaanxi Province of China(2021KJXX-39)

短肽自组装水凝胶作为一种新型的生物材料,具有生物相容性高、免疫原性低、含水量高、降解产物可被机体重吸收利用、结构与天然细胞外基质类似等优点,使其在材料科学、生物医药及临床医学等领域具有广阔的应用前景。在这篇综述中,我们主要介绍了常用的几种制备稳定的肽自组装水凝胶方法,包括酶催化的水凝胶化、化学/物理交联的水凝胶化以及光催化的水凝胶化。进一步,我们介绍一些关于肽自组装水凝胶在药物递送和抗肿瘤治疗、抗菌和伤口愈合以及3D生物打印和组织工程中的应用。我们希望通过本文的论述能引起更多的人对肽自组装水凝胶的关注,以推进其在生物医学领域应用的发展。

关键词： 肽自组装, 水凝胶, 药物递送, 抗菌, 组织工程

As a new type of biological material, short peptide self-assembled hydrogel holds the advantages of high biocompatibility, low immunogenicity, high water content, degradation products that can be absorbed by the body, and structure similar to natural extracellular matrix. It has broad application prospects in the fields of materials science, biomedicine and clinical medicine. In this review, we mainly introduce several commonly used methods for preparing stable and tough peptide self-assembled hydrogels, including enzyme-catalyzed hydrogelation and chemical/physical cross-linked hydrogelation and photocatalytic hydrogelation. Furthermore, some applications of peptide self-assembled hydrogels in drug delivery and anti-tumor therapy, antibacterial and wound healing, 3D bioprinting and tissue engineering are introduced. We hope that the discussion in this review can arouse more people's attention to peptide self-assembled hydrogels and promote the development of their applications in the biomedical field.

Contents

1 Introduction

2 Preparation of peptide self-assembled hydrogel

2.1 Enzyme-catalyzed hydrogelation

2.2 Chemical/physical cross-linked hydrogelation

2.3 Photocatalytic hydrogelation

3 Biomedical applications

3.1 Drug delivery and anti-tumor therapy

3.2 Antibacterial and wound healing

3.3 3D bioprinting and tissue engineering

4 Conclusion and perspective

Key words: peptide self-assembly, hydrogel, drug delivery, antibacterial, tissue engineering
()
图1 离子诱导酶网络结构的改变调控肽的组装结构示意图[49]
Fig.1 Schematic diagram of the regulation of peptide assembled structure by changing the structure of the ion-induced enzyme network[49]
图2 A) 肽分子结构式;B)水凝胶形貌表征图;C) 水凝胶可注射性表征;D) 3D打印水凝胶支架[54]
Fig.2 A) Peptide molecular structure; B) Morphology characterization of the hydrogel; C) Injectability characterization of the hydrogel; D) 3D printed hydrogel scaffold[54]
图3 Ru ( b p y ) 3 2 +介导的含酪氨酸短肽的光致化学交联以制备稳定性增强的肽基水凝胶示意图[59]
Fig.3 Schematic of Ru ( b p y ) 3 2 + mediated photocross-linking of tyrosine contained peptide for hydrogel formation with enhanced mechanical stability[59]
表1 肽自组装水凝胶制备方法汇总表
Table 1 Summary table of preparation methods for self-assembled peptide hydrogels
Peptide molecular species Crosslinking
methods		 ref
Fmoc-Phe /(Phe)2 Thermolysin 45
Fmoc-YL-OMe Subtilisin 49
Ac-YYYpY-OMe Tyrosinase 44
CRB-GDFDFpDY Alkaline phosphatase 50
Fmoc- (Phe)3 Genipin 52
LIVAGKC Cysteine disulfide bond 53
Nap-YYF PEGMA 54
Fmoc-FF/PLL-SH Disulfide bond 56
Short peptide hyaluronic acid complex UV 57
YYAYY White light 60
Fmoc-FFEEK(D)GGY Visible light 61,62
图4 A) 肽分子结构式;B) G3 (G(IIKK)3-NH2) 在含与不含MMP-2的Ac-I3SLGK-NH2凝胶中的释放情况;C) NIH 3T3 和 HeLa 细胞在负载和未负载 G3 的两种肽凝胶上孵育 24 h 后的存活率[65]
Fig.4 A) Molecular structures of the designed peptides; B) G3 (G(IIKK)3-NH2) release from the G3-loaded Ac-I3SLGK-NH2+MMP2 and Ac-I3SLGK-NH2 gels in the presence and absence of MMP-2; C) Viability of NIH 3T3 and HeLa cells on the two peptide gels with and without G3 loading. After incubation for 24 h, the cell viability was determined by MTT assays[65]
图5 A) 静电相互作用驱动的Fmoc-FF/PLL-SH共组装形成水凝胶的示意图; B) 在有或没有 Fmoc-FF/PLL-SH 水凝胶处理的情况下的肿瘤组织生长情况,其中插图为水凝胶植入12 天后切除的 B16 肿瘤组织的照片; C) 不同处理后 CD4+T/CD8+T 的比值[56]
Fig.5 A) Schematic diagram of the formation of Fmoc-FF/PLL-SH hydrogels based on electrostatic interaction-triggered co-assembly; B) Suppression of tumor growth with or without Fmoc-FF/PLL-SH hydrogel treatment. Images of the excised B16 tumor tissues at 12 days post implantation (inset); C) The ratio of CD4+T/CD8+T after different treatment[56]
图6 肽A9K2自组装水凝胶选择性抗菌示意图[88]
Fig.6 Schematic diagram of peptide A9K2 self-assembled hydrogel for antibacterial applications[88]
图7 全层烧伤模型示意图:A) 将预热的铝板涂在麻醉小鼠剃光的背上;然后去除受伤的皮肤并涂抹水凝胶; B) 水凝胶植入伤口部位的示意图[92]
Fig.7 Schematic representation of the full-thickness burn wound model: A) A pre-heated aluminum plaque was applied to the shaved dorsum of the anesthetized mouse; followed by the removal of the injured skin and the application of the hydrogel. B) A schematic of the hydrogel-implanted wound site[92]
图8 A) 脂肪族超短肽的分子结构; B)肽自组装水凝胶可连续注射性能表征; C)通过连续沉积肽溶液和含有细胞和/或小分子的 PBS 缓冲液打印的液滴阵列;D)在水凝胶中培养人胚胎干细胞H1[96]
Fig.8 A) Molecular structure of aliphatic ultrashort peptides; B) characterization of continuous injectable properties of peptide assembled hydrogel by extruding hydrogel into a concentrated salt bath; C) printed droplet arrays via sequential deposition of peptide and PBS containing cells and/or small molecules; D) Human H1 embryonic stem cells encapsulated in the hydrogels[96]
图9 肽组装水凝胶中碳纳米管的存在对细胞行为影响的示意图[98]
Fig.9 Effect of presence of SWNTs in the peptide assembled hydrogel on the cell behavior[98]
[1]
van den Ent F, Amos L A, Löwe J. Nature, 2001, 413(6851): 39.

doi: 10.1038/35092500     URL    
[2]
Salgado E N, Radford R J, Tezcan F A. Acc. Chem. Res., 2010, 43(5): 661.

doi: 10.1021/ar900273t     URL    
[3]
Qi G B, Gao Y J, Wang L, Wang H. Adv. Mater., 2018, 30(22): 1703444.

doi: 10.1002/adma.201703444     URL    
[4]
Abbas M, Zou Q L, Li S K, Yan X H. Adv. Mater., 2017, 29(12): 1605021.

doi: 10.1002/adma.201605021     URL    
[5]
Tao K, Levin A, Adler-Abramovich L, Gazit E. Chem. Soc. Rev., 2016, 45(14): 3935.

doi: 10.1039/C5CS00889A     URL    
[6]
Wang J, Liu K, Xing R R, Yan X H. Chem. Soc. Rev., 2016, 45(20): 5589.

doi: 10.1039/C6CS00176A     URL    
[7]
Han J J, Gong H N, Ren X K, Yan X H. Nano Today, 2021, 41: 101295.

doi: 10.1016/j.nantod.2021.101295     URL    
[8]
Bai S, Debnath S, Javid N, Frederix P W J M, Fleming S, Pappas C, Ulijn R V. Langmuir, 2014, 30(25): 7576.

doi: 10.1021/la501335e     URL    
[9]
Wang A H, Cui L Y, Debnath S, Dong Q Q, Yan X H, Zhang X, Ulijn R V, Bai S. ACS Appl. Mater. Interfaces, 2017, 9(25): 21390.

doi: 10.1021/acsami.7b05661     URL    
[10]
Yan X H, Zhu P L, Li J B. Chem. Soc. Rev., 2010, 39(6): 1877.

doi: 10.1039/b915765b     URL    
[11]
Zou Q L, Zhang L, Yan X H, Wang A H, Ma G H, Li J B, Möhwald H, Mann S. Angew. Chem. Int. Ed., 2014, 53(9): 2366.

doi: 10.1002/anie.201308792     URL    
[12]
Liu X C, Zhu P L, Fei J B, Zhao J, Yan X H, Li J B. Chem. Eur. J., 2015, 21(26): 9461.

doi: 10.1002/chem.201500580     URL    
[13]
Lam K S, Salmon S E, Hersh E M, Hruby V J, Kazmeierski W M, Knapp R J. Nature, 1992, 360(6406): 768.
[14]
Zhang H, Fei J B, Yan X H, Wang A H, Li J B. Adv. Funct. Mater., 2015, 25(8): 1193.

doi: 10.1002/adfm.201403119     URL    
[15]
Wu J H, Chen A P, Qin M, Huang R, Zhang G, Xue B, Wei J W, Li Y, Cao Y, Wang W. Nanoscale, 2015, 7(5): 1655.

doi: 10.1039/C4NR05798H     URL    
[16]
Ma K, Xing R R, Jiao T F, Shen G Z, Chen C J, Li J B, Yan X H. ACS Appl. Mater. Interfaces, 2016, 8(45): 30759.

doi: 10.1021/acsami.6b10754     URL    
[17]
Cui L Y, Jiao Y, Wang A H, Zhao L Y, Dong Q Q, Yan X H, Bai S. Chem. Commun., 2018, 54(18): 2208.

doi: 10.1039/C8CC00177D     URL    
[18]
Feng L, Wang A H, Ren P, Wang M Y, Dong Q Q, Li J L, Bai S. Colloid Interface Sci. Commun., 2018, 23: 29.

doi: 10.1016/j.colcom.2018.01.006     URL    
[19]
Sun J, Guo Y, Xing R, Jiao T, Zou Q, Yan X. Colloids Surf. A, 2017, 514, 155.

doi: 10.1016/j.colsurfa.2016.11.062     URL    
[20]
Zou Q L, Liu K, Abbas M, Yan X H. Adv. Mater., 2016, 28(6): 1031.

doi: 10.1002/adma.201502454     URL    
[21]
Liu K, Xing R R, Zou Q L, Ma G H, Möhwald H, Yan X H. Angew. Chem. Int. Ed., 2016, 55(9): 3036.

doi: 10.1002/anie.201509810     URL    
[22]
Liu K, Xing R, Chen C, Shen G, Yan L, Zou Q, Ma G, Moehwald H, Yan X. Angew. Chem. Int. Edit., 2015, 54 (2): 500.
[23]
Liu K, Yuan C Q, Zou Q L, Xie Z C, Yan X H. Angew. Chem. Int. Ed., 2017, 56(27): 7876.

doi: 10.1002/anie.201704678     URL    
[24]
Yan X H, Li J B, Möhwald H. Adv. Mater., 2011, 23(25): 2796.

doi: 10.1002/adma.201100353     URL    
[25]
Du X W, Zhou J, Shi J F, Xu B. Chem. Rev., 2015, 115(24): 13165.

doi: 10.1021/acs.chemrev.5b00299     URL    
[26]
Jonker A M, Löwik D W P M, van Hest J C M. Chem. Mater., 2012, 24(5): 759.

doi: 10.1021/cm202640w     URL    
[27]
Li Y, Qin M, Cao Y, Wang W. Sci. China Phys. Mech. Astron., 2014, 57(5): 849.

doi: 10.1007/s11433-014-5427-z     URL    
[28]
Wang H M, Yang Z M. Nanoscale, 2012, 4(17): 5259.

doi: 10.1039/c2nr31149f     URL    
[29]
Fu M, Zhang C Y, Dai Y X, Li X, Pan M B, Huang W L, Qian H, Ge L. Biomater. Sci., 2018, 6(6): 1480.

doi: 10.1039/C8BM00006A     URL    
[30]
Yu S J, Zhang D L, He C L, Sun W J, Cao R J, Cui S S, Deng M X, Gu Z, Chen X S. Biomacromolecules, 2017, 18(12): 4341.

doi: 10.1021/acs.biomac.7b01374     URL    
[31]
Li X, Fu M, Wu J, Zhang C Y, Deng X, Dhinakar A, Huang W L, Qian H, Ge L. Acta Biomater., 2017, 51: 294.

doi: 10.1016/j.actbio.2017.01.016     URL    
[32]
Wan S, Borland S, Richardson S M, Merry C L R, Saiani A, Gough J E. Acta Biomater., 2016, 46: 29.

doi: 10.1016/j.actbio.2016.09.033     URL    
[33]
Koss K M, Unsworth L D. Acta Biomater., 2016, 44: 2.

doi: 10.1016/j.actbio.2016.08.026     pmid: 27544809
[34]
Takeuchi T, Bizenjima T, Ishii Y, Imamura K, Suzuki E, Seshima F, Saito A. J. Clin. Periodontol., 2016, 43(3): 279.

doi: 10.1111/jcpe.12515     URL    
[35]
Paladini F, Meikle S T, Cooper I R, Lacey J, Perugini V, Santin M. J. Mater. Sci. Mater. Med., 2013, 24(10): 2461.

doi: 10.1007/s10856-013-4986-2     URL    
[36]
Altunbas A, Lee S J, Rajasekaran S A, Schneider J P, Pochan D J. Biomaterials, 2011, 32(25): 5906.

doi: 10.1016/j.biomaterials.2011.04.069     pmid: 21601921
[37]
Semino C E, Kasahara J, Hayashi Y, Zhang S G. Tissue Eng., 2004, 10(3/4): 643.

doi: 10.1089/107632704323061997     URL    
[38]
Koutsopoulos S, Zhang S G. Acta Biomater., 2013, 9(2): 5162.

doi: 10.1016/j.actbio.2012.09.010     pmid: 22995405
[39]
Huang Z, Sargeant T D, Hulvat J F, Mata A, Bringas P, Koh C Y, Stupp S I, Snead M L. J. Bone Miner. Res., 2008, 23(12): 1995.

doi: 10.1359/jbmr.080705     pmid: 18665793
[40]
Silva G A, Czeisler C, Niece K L, Beniash E, Harrington D A, Kessler J A, Stupp S I. Science, 2004, 303(5662): 1352.

doi: 10.1126/science.1093783     URL    
[41]
Standley S M, Toft D J, Cheng H, Soukasene S, Chen J, Raja S M, Band V, Band H, Cryns V L, Stupp S I. Cancer Res., 2010, 70(8): 3020.

doi: 10.1158/0008-5472.CAN-09-3267     URL    
[42]
Soukasene S, Toft D J, Moyer T J, Lu H, Lee H K, Standley S M, Cryns V L, Stupp S I. ACS Nano, 2011, 5(11): 9113.

doi: 10.1021/nn203343z     pmid: 22044255
[43]
Gahane A Y, Ranjan P, Singh V, Sharma R K, Sinha N, Sharma M, Chaudhry R, Thakur A K. Soft Matter, 2018, 14(12): 2234.

doi: 10.1039/C7SM02317K     URL    
[44]
Gao J, Zheng W T, Kong D L, Yang Z M. Soft Matter, 2011, 7(21): 10443.

doi: 10.1039/c1sm06192e     URL    
[45]
Toledano S, Williams R J, Jayawarna V, Ulijn R V. J. Am. Chem. Soc., 2006, 128(4): 1070.

pmid: 16433511
[46]
Wang H M, Feng Z, Xu B. Chem. Soc. Rev., 2017, 46(9): 2421.

doi: 10.1039/C6CS00656F     URL    
[47]
Feng Z, Zhang T F, Wang H M, Xu B. Chem. Soc. Rev., 2017, 46(21): 6470.

doi: 10.1039/C7CS00472A     URL    
[48]
Hughes M, Debnath S, Knapp C W, Ulijn R V. Biomater. Sci., 2013, 1(11): 1138.

doi: 10.1039/c3bm60135h     URL    
[49]
Roy S, Javid N, Sefcik J, Halling P J, Ulijn R V. Langmuir, 2012, 28(48): 16664.

doi: 10.1021/la303388s     URL    
[50]
Liang C H, Zheng D B, Shi F, Xu T Y, Yang C H, Liu J F, Wang L, Yang Z M. Nanoscale, 2017, 9(33): 11987.

doi: 10.1039/C7NR04370H     URL    
[51]
Liyanage W, Ardoña H A M, Mao H Q, Tovar J D. Bioconjugate Chem., 2017, 28(3): 751.

doi: 10.1021/acs.bioconjchem.6b00593     URL    
[52]
Chronopoulou L, Daniele M, Perez V, Gentili A, Gasperi T, Lupi S, Palocci C. Process. Biochem., 2018, 70: 110.

doi: 10.1016/j.procbio.2018.04.005     URL    
[53]
Seow W Y, Salgado G, Lane E B, Hauser C A E. Sci. Rep., 2016, 6: 32670.

doi: 10.1038/srep32670     URL    
[54]
Wei Q C, Xu M C, Liao C N, Wu Q, Liu M Y, Zhang Y, Wu C T, Cheng L M, Wang Q G. Chem. Sci., 2016, 7(4): 2748.

doi: 10.1039/C5SC02234G     URL    
[55]
Fernández-Muiños T, Recha-Sancho L, López-Chicón P, Castells-Sala C, Mata A, Semino C E. Acta Biomater., 2015, 16: 35.

doi: 10.1016/j.actbio.2015.01.008     pmid: 25595471
[56]
Xing R R, Li S K, Zhang N, Shen G Z, Möhwald H, Yan X H. Biomacromolecules, 2017, 18(11): 3514.

doi: 10.1021/acs.biomac.7b00787     URL    
[57]
Li R X, Sun Y, Cai Z W, Li Y, Sun J, Bi W, Yang F, Zhou Q R, Ye T J, Yu Y C. Chem. Eng. J., 2021, 415: 129015.

doi: 10.1016/j.cej.2021.129015     URL    
[58]
Kim S H, Sun Y, Kaplan J A, Grinstaff M W, Parquette J R. New J. Chem., 2015, 39(5): 3225.

doi: 10.1039/C5NJ00038F     URL    
[59]
Ding Y, Li Y, Qin M, Cao Y, Wang W. Langmuir, 2013, 29(43): 13299.

doi: 10.1021/la4029639     pmid: 24090141
[60]
Min K I, Kim D H, Lee H J, Lin L W, Kim D P. Angew. Chem. Int. Ed., 2018, 57(20): 5630.

doi: 10.1002/anie.201713261     URL    
[61]
Kim I, Bang W Y, Park W H, Han E H, Lee E. Nanoscale, 2019, 11(37): 17327.

doi: 10.1039/C9NR06115K     URL    
[62]
Liu C, Hua J C, Ng P F, Fei B. J. Mater. Sci. Technol., 2021, 63: 182.

doi: 10.1016/j.jmst.2020.02.086     URL    
[63]
Karavasili C, Panteris E, Vizirianakis I S, Koutsopoulos S, Fatouros D G. Pharm. Res., 2018, 35(8): 1.
[64]
Liu J, Zhang L, Yang Z, Zhao X. Int. J. Nanomed., 2011, 6:2143.
[65]
Chen C X, Zhang Y, Hou Z, Cui X J, Zhao Y R, Xu H. Biomacromolecules, 2017, 18(11): 3563.

doi: 10.1021/acs.biomac.7b00911     URL    
[66]
Jiang T Y, Wang T, Li T, Ma Y D, Shen S Y, He B F, Mo R. ACS Nano, 2018, 12(10): 9693.

doi: 10.1021/acsnano.8b03800     URL    
[67]
Abbas M, Xing R R, Zhang N, Zou Q L, Yan X H. ACS Biomater. Sci. Eng., 2018, 4(6): 2046.

doi: 10.1021/acsbiomaterials.7b00624     URL    
[68]
Wang H M, Feng Z, Wu D D, Fritzsching K J, Rigney M, Zhou J, Jiang Y J, Schmidt-Rohr K, Xu B. J. Am. Chem. Soc., 2016, 138(34): 10758.

doi: 10.1021/jacs.6b06075     URL    
[69]
Wang H M, Feng Z, Wang Y Z, Zhou R, Yang Z M, Xu B. J. Am. Chem. Soc., 2016, 138(49): 16046.

doi: 10.1021/jacs.6b09783     URL    
[70]
Cai Y B, Shen H S, Zhan J, Lin M L, Dai L H, Ren C H, Shi Y, Liu J F, Gao J, Yang Z M. J. Am. Chem. Soc., 2017, 139(8): 2876.

doi: 10.1021/jacs.6b12322     URL    
[71]
Zhan J, Cai Y B, He S S, Wang L, Yang Z M. Angew. Chem. Int. Ed., 2018, 57(7): 1813.

doi: 10.1002/anie.201710237     pmid: 29276818
[72]
Liu Y, Xu Y Y, Tian Y, Chen C Y, Wang C, Jiang X Y. Small, 2014, 10(22): 4505.

doi: 10.1002/smll.201401707     pmid: 25238620
[73]
Hubbell J A, Thomas S N, Swartz M A. Nature, 2009, 462(7272): 449.

doi: 10.1038/nature08604     URL    
[74]
Petersen L K, Ramer-Tait A E, Broderick S R, Kong C S, Ulery B D, Rajan K, Wannemuehler M J, Narasimhan B. Biomaterials, 2011, 32(28): 6815.

doi: 10.1016/j.biomaterials.2011.05.063     URL    
[75]
He Q, Mitchell A R, Johnson S L, Wagner-Bartak C, Morcol T, Bell S J D. Clin. Diagn. Lab. Immunol., 2000, 7(6): 899.

doi: 10.1128/CDLI.7.6.899-903.2000     pmid: 11063495
[76]
de Temmerman M L, Rejman J, Demeester J, Irvine D J, Gander B, de Smedt S C. Drug Discov. Today, 2011, 16(13/14): 569.

doi: 10.1016/j.drudis.2011.04.006     URL    
[77]
Luo Z C, Li P, Deng J Z, Gao N N, Zhang Y J, Pan H, Liu L L, Wang C, Cai L T, Ma Y F. J. Control. Release, 2013, 170(2): 259.

doi: 10.1016/j.jconrel.2013.05.027     URL    
[78]
Delgado M, Lee K J, Altobell , Spanka C, Wentworth P, Janda K D. J. Am. Chem. Soc., 2002, 124(18): 4946.

doi: 10.1021/ja025715b     URL    
[79]
Wu Y B, Wei W, Zhou M, Wang Y Q, Wu J, Ma G H, Su Z G. Biomaterials, 2012, 33(7): 2351.

doi: 10.1016/j.biomaterials.2011.11.068     URL    
[80]
Singh A, Peppas N A. Adv. Mater., 2014, 26(38): 6530.

doi: 10.1002/adma.201402105     URL    
[81]
Rudra J S, Tian Y F, Jung J P, Collier J H. PNAS, 2010, 107(2): 622.

doi: 10.1073/pnas.0912124107     pmid: 20080728
[82]
Huang Z H, Shi L, Ma J W, Sun Z Y, Cai H, Chen Y X, Zhao Y F, Li Y M. J. Am. Chem. Soc., 2012, 134(21): 8730.

doi: 10.1021/ja211725s     URL    
[83]
Wang H M, Luo Z C, Wang Y Z, He T, Yang C B, Ren C H, Ma L S, Gong C Y, Li X Y, Yang Z M. Adv. Funct. Mater., 2016, 26(11): 1822.

doi: 10.1002/adfm.201505188     URL    
[84]
Liu Y H, Wang Y Z, Yu F, Zhang Z Q, Yang Z M, Zhang W P, Wang P G, Zhao W. Chem. Commun., 2017, 53(68): 9486.

doi: 10.1039/C7CC04386D     URL    
[85]
Malhotra K, Shankar S, Rai R, Singh Y. Biomacromolecules, 2018, 19(3): 782.

doi: 10.1021/acs.biomac.7b01582     pmid: 29384665
[86]
Nandi N, Gayen K, Ghosh S, Bhunia D, Kirkham S, Sen S K, Ghosh S, Hamley I W, Banerjee A. Biomacromolecules, 2017, 18(11): 3621.

doi: 10.1021/acs.biomac.7b01006     URL    
[87]
Wan Y M, Liu L B, Yuan S S, Sun J, Li Z B. Langmuir, 2017, 33(13): 3234.

doi: 10.1021/acs.langmuir.6b03986     URL    
[88]
Bai J K, Chen C X, Wang J X, Zhang Y, Cox H, Zhang J, Wang Y M, Penny J, Waigh T, Lu J R, Xu H. ACS Appl. Mater. Interfaces, 2016, 8(24): 15093.

doi: 10.1021/acsami.6b03770     URL    
[89]
Bai J K, Gong Z Y, Wang J X, Wang C D. RSC Adv., 2017, 7(77): 48631.

doi: 10.1039/C7RA09743C     URL    
[90]
Carrejo N C, Moore A N, Lopez Silva T L, Leach D G, Li I C, Walker D R, Hartgerink J D. ACS Biomater. Sci. Eng., 2018, 4(4): 1386.

doi: 10.1021/acsbiomaterials.8b00031     pmid: 29687080
[91]
Loo Y, Wong Y C, Cai E Z, Ang C H, Raju A, Lakshmanan A, Koh A G, Zhou H J, Lim T C, Moochhala S M, Hauser C A E. Biomaterials, 2014, 35(17): 4805.

doi: 10.1016/j.biomaterials.2014.02.047     URL    
[92]
Yergoz F, Hastar N, Cimenci C E, Ozkan A D, Tekinay T, Guler M O, Tekinay A B. Biomaterials, 2017, 134: 117.

doi: S0142-9612(17)30277-6     pmid: 28458029
[93]
Raphael B, Khalil T, Workman V L, Smith A, Brown C P, Streuli C, Saiani A, Domingos M. Mater. Lett., 2017, 190: 103.

doi: 10.1016/j.matlet.2016.12.127     URL    
[94]
Sun W X, Xue B, Li Y, Qin M, Wu J Y, Lu K, Wu J H, Cao Y, Jiang Q, Wang W. Adv. Funct. Mater., 2016, 26(48): 9044.

doi: 10.1002/adfm.201603512     URL    
[95]
Li L, Zhang K J, Wang T K, Wang P, Xue B, Cao Y, Zhu L Y, Jiang Q. Mater. Des., 2020, 189: 108492.

doi: 10.1016/j.matdes.2020.108492     URL    
[96]
Loo Y, Lakshmanan A, Ni M, Toh L L, Wang S, Hauser C A E. Nano Lett., 2015, 15(10): 6919.

doi: 10.1021/acs.nanolett.5b02859     URL    
[97]
Hoffman A S. Adv. Drug Deliv. Rev., 2012, 64: 18.

doi: 10.1016/j.addr.2012.09.010     URL    
[98]
Sheikholeslam M, Wheeler S D, Duke K G, Marsden M, Pritzker M, Chen P. Acta Biomater., 2018, 69: 107.

doi: S1742-7061(17)30768-7     pmid: 29248638
[99]
Cheng B C, Yan Y F, Qi J J, Deng L F, Shao Z W, Zhang K Q, Li B, Sun Z L, Li X M. ACS Appl. Mater. Interfaces, 2018, 10(15): 12474.

doi: 10.1021/acsami.8b01725     URL    
[1] 陈一明, 李慧颖, 倪鹏, 方燕, 刘海清, 翁云翔. 含儿茶酚基团的湿态组织粘附水凝胶[J]. 化学进展, 2023, 35(4): 560-576.
[2] 李良春, 郑仁林, 黄毅, 孙荣琴. 多组分自组装小分子水凝胶中的自分类组装[J]. 化学进展, 2023, 35(2): 274-286.
[3] 宫悦, 程一竹, 胡银春. 高分子导电水凝胶的制备及在柔性可穿戴电子设备中的应用[J]. 化学进展, 2022, 34(3): 616-629.
[4] 牛小连, 刘柯君, 廖子明, 徐慧伦, 陈维毅, 黄棣. 基于骨组织工程的静电纺纳米纤维[J]. 化学进展, 2022, 34(2): 342-355.
[5] 郑明心, 谭臻至, 袁金颖. 光响应Janus粒子体系的构建与应用[J]. 化学进展, 2022, 34(11): 2476-2488.
[6] 漆晨阳, 涂晶. 无抗生素纳米抗菌剂:现状、挑战与展望[J]. 化学进展, 2022, 34(11): 2540-2560.
[7] 荆晓东, 孙莹, 于冰, 申有青, 胡浩, 丛海林. 肿瘤微环境响应药物递送系统的设计[J]. 化学进展, 2021, 33(6): 926-941.
[8] 李立清, 吴盼旺, 马杰. 双网络凝胶吸附剂的构建及其去除水中污染物的应用[J]. 化学进展, 2021, 33(6): 1010-1025.
[9] 许惠凤, 董永强, 朱希, 余丽双. 新型二维材料MXene在生物医学的应用[J]. 化学进展, 2021, 33(5): 752-766.
[10] 杨宇州, 李政, 黄艳凤, 巩继贤, 乔长晟, 张健飞. MOF基水凝胶材料的制备及其应用[J]. 化学进展, 2021, 33(5): 726-739.
[11] 李超, 乔瑶雨, 李禹红, 闻静, 何乃普, 黎白钰. MOFs/水凝胶复合材料的制备及其应用研究[J]. 化学进展, 2021, 33(11): 1964-1971.
[12] 张开宇, 高国伟, 李延生, 宋钰, 温永强, 张学记. DNA水凝胶在生物传感中的应用和发展[J]. 化学进展, 2021, 33(10): 1887-1899.
[13] 吴晴, 唐一源, 余淼, 张悦莹, 李杏梅. 基于肿瘤微环境响应的DNA纳米结构递药系统[J]. 化学进展, 2020, 32(7): 927-934.
[14] 薛一凡, 孟文卉, 汪润泽, 任俊杰, 衡伟利, 张建军. 过饱和度理论及过饱和药物递送系统[J]. 化学进展, 2020, 32(6): 698-712.
[15] 刘阳, 张新波, 赵樱灿. 二维MoS2纳米材料及其复合物在水处理中的应用[J]. 化学进展, 2020, 32(5): 642-655.