English
往期目录
新闻公告
More
化学进展 2020, Vol. 32 Issue (6): 687-697 DOI: 10.7536/PC191020   后一篇

• 综述与评论 •

DNA-多肽复合分子的设计、组装与应用

王子瑄1, 王跃飞1,2,**(), 齐崴1,2,3, 苏荣欣1,2,3, 何志敏1   

  1. 1. 天津大学化工学院 天津 300072
    2. 天津大学化学工程联合国家重点实验室 天津 300072
    3. 天津化学化工协同创新中心 天津 300072
  • 收稿日期:2019-10-29 修回日期:2020-01-19 出版日期:2020-06-05 发布日期:2020-04-13
  • 通讯作者: 王跃飞
  • 作者简介:
    ** Corresponding author e-mail:
  • 基金资助:
    国家自然科学基金项目(21606166, 21621004, 51773149)
Richhtml ( 89 ) 下载PDF ( 1184 ) 引用
导出

Design, Self-Assembly and Application of DNA-Peptide Hybrid Molecules

Zixuan Wang1, Yuefei Wang1,2,**(), Wei Qi1,2,3, Rongxin Su1,2,3, Zhimin He1   

  1. 1. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
    2. State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin 300072, China
    3. Collaborative Innovation Center of Chemical Science and Engineering(Tianjin), Tianjin 300072, China;
  • Received:2019-10-29 Revised:2020-01-19 Online:2020-06-05 Published:2020-04-13
  • Contact: Yuefei Wang
  • Supported by:
    the National Natural Science Foundation of China(21606166, 21621004, 51773149)

DNA-多肽复合分子作为一类新型的自组装分子受到研究人员的广泛关注。DNA分子具有可编程性、高特异性、功能多样等优点,多肽分子是一类重要的生物小分子,能够通过分子自组装形成具有不同结构的纳米材料,因此,将二者通过共价交联,可以获得具有多级自组装行为的DNA-多肽复合分子,能够实现两类重要生物分子功能的集成优化,合成具有不同结构与功能的超分子自组装材料。此外,通过酶催化、DNA杂化、DNA链置换反应等,还可实现对多肽-DNA复合分子自组装行为的动态调控,进而模拟生命系统中复杂动态的自组装结构,强化相关材料在生物、化学、材料等领域的应用。本文讨论了DNA-多肽复合分子的设计、组装与应用方面的最新进展,最后基于目前DNA-多肽复合分子存在的一些问题对DNA-多肽复合分子的研究做了展望。

关键词： DNA-多肽复合分子, 自组装, 动态调控, 功能材料, 生物医药

As a new kind of self-assembled molecules, DNA-peptide hybrid molecules have attracted great interests. DNA has the advantages of programmability, high specificity and versatility. Peptides are a kind of important biological small molecules, which can form nanomaterials with various structures through molecular self-assembly. Therefore, DNA and peptide can be cross-linked with each other to form DNA-peptide hybrid molecules with hierarchical self-assembly behavior. In this way, the integration of the functions of two important biomolecules can be realized, and the supramolecular materials with different structures and functions can be assembled. Moreover, through the combination of enzyme catalysis, DNA hybridization, and strand displacement reactions, we are able to control the dynamic self-assembly of the peptide-DNA conjugates. This allows us to mimic the complex dynamic structures existing in biological systems, which will have great potential applications in biology, chemistry and materials science. In this paper, the latest progress in the design, self-assembly and applications of DNA-polypeptide hybrid molecules are reviewed. Some problems with regard to DNA-polypeptide molecules are summarized, and the prospect of the research on DNA-polypeptide molecules is made.

Contents

1 Introduction
2 Design of DNA-peptide hybrid molecules
3 Assembly and regulation of DNA-peptide hybrid molecules

3.1 Self-assembly mechanism

3.2 Supramolecular self-assembly of DNA-polypeptide hybrid molecules

3.3 Dynamic self-assembly of DNA-amphiphilic peptide molecules

4 Applications of functional materials assembled by DNA-peptide hybrid molecules

4.1 Biomedical applications

4.2 3D bioprinting

5 Conclusion and outlook
Key words: DNA-peptide molecules, self-assembly, dynamic control, functional materials, biomedical applications
()
表1 DNA-多肽复合分子的设计、组装与应用
Table 1 Design, self-assembly and application of DNA-peptide hybrid molecules
Molecular Structure Method Assembly principle Application ref
DNA-polypeptide hybrid molecules Ring-opening polymerization and Click chemistry DNA hybridization Hydrogels 35
Ring-opening polymerization and Click chemistry DNA hybridization 3D bioprinting 3740
DNA-amphiphilic peptide molecules Click chemistry Peptide self-assembly, DNA hybridization, and strand displacement reactions Biomedical applications 18
Click chemistry Peptide self-assembly, DNA hybridization \ 38
Solid-phase synthesis Base pairing \ 39
Click chemistry Peptide self-assembly Biomedical applications 36
peptide C-9R-C, peptide C-RGD-C Ligand-to-metal charge transfer \ Biomedical applications 42
图1 (a)DNA与短肽复合分子以及DNA与聚合物多肽复合分子的结构示意图;(b)两种将功能基团引入DNA-多肽复合分子的方法;(c)PPLG-g-DNA 分子刷的合成路线[35];(d)用双功能交联剂合成DNA-肽复合物[43];(e)环辛炔修饰的寡核苷酸与叠氮基-赖氨酸封端的两亲性多肽之间的交联反应[36];(f)利用配体-金属电荷转移将功能肽与DNA分子结构[42]
Fig. 1 (a) DNA-short peptide hybrid molecule and DNA-polypeptide hybrid molecule; (b) Two methods of introducing functional groups into DNA-peptide molecules; (c) Synthetic routes of PPLG-g-DNA molecular brush[35]; (d) Synthesis of DNA-peptide conjugates using a bifunctional cross-linker[43]; (e) Crosslinking reaction between cyclooctyne modified oligonucleotide and azido-lysine-terminated PA.[36]; (f) Direct incorporation of functional peptides with M-DNA through ligand-to-metal charge transfer[42]
图2 (a,b)多肽α-螺旋与β-折叠片二级结构[54];(c)两亲性多肽自组装示意图[54];(d)DNA链置换反应示意图及其位移动力学[53]
Fig. 2 (a,b) Schematic diagram of the α-helix and β-sheet secondary structures of the peptides[54]; (c) Schematic illustration showing the self-assembly of amphiphilic peptides[54]; (d) Schematic diagram of the DNA chain displacement reaction and its dynamics[53]
图3 (a)具有多修饰位点的多肽-DNA水凝胶的形成机理;(b)水凝胶自愈合示意图;(c)水凝胶的可注射性;(d)聚(γ-丙炔基-L-谷氨酸)与叠氮化物-DNA反应制备分子刷,再分别和ds-DNA、DNA交联剂交联[35]
Fig. 3 (a) The formation mechanism of the polypeptide-DNA hydrogel with rationally designed multi-modification sites; (b) The schematic mechanism of the self-healing and fusion behaviors of the DNA-peptide hydrogels; (c) Injectability of the hydrogels; (d) γ-propargyl-L-glutamate N-carboxyanhydride(PLG-NCA) reacts with azide-functionalized DNA strands to synthesize molecular brushes, which are then crosslinked with ds-DNA and DNA crosslinker, respectively[35]
图4 (a)DNA-PA复合分子与PA共组装成纳米纤维;(b)PA分子与PA-DNA复合分子示意图;(c)两根由DNA-两亲性多肽自组装形成的纳米纤维通过表面互补DNA链的杂化反应进行交联;(d)纤维束生长速率是纤维内(E intra)和纤维间能量(E inter)的函数;(e)可逆凝胶的扫描电镜显微照片,比例尺:10 μm[18]
Fig. 4 (a) Schematic diagram of PA molecule and PA-DNA molecule; (b) Nanofibers formed by co-assembly of PA with DNA-PA molecules; (c) Illustration of peptide amphiphile fibers cross-linked by DNA hybridization; fibers are shown in their initial state prior to monomer exchange; (d) Bundle growth rate as a function of intra- and interfiber energies(E intra, E inter); (e) SEM images showing the reversible fiber structures during heating-cooling cycle or by addition of DNA invader and anti-invader, scale: 10 μm[18]
图5 (a)多肽-DNA复合纤维之间通过DNA链之间的杂化反应进行交联,形成纤维束结构;(b)纤维束结构的TEM图像;(c)升高温度之后,多肽-DNA纤维束解聚为互相独立的单根纳米纤维的TEM图像;(d)通过DNA链置换反应,诱导多肽-DNA纤维束解聚为互相独立的单根纳米纤维的TEM图像;e)在提高pH前后与添加DNA消化酶前后多肽-DNA分子及其组装体结构的TEM照片[38]
Fig. 5 (a) Schematic of fiber bundling with DNA handles; (b) TEM image of the annealed complementary assembly; (c) Schematic and TEM image of the heated complementary pepDNA assembly; (d) TEM image and schematic of the strand displacement reaction products; (e) Molecular graphics representation and corresponding morphologies observed by TEM of pep-DNA before and after raising the pH or adding DNase to digest the DNA block[38]
图6 (a)核肽的聚集状态可通过酶催化ATP与ADP的转化进行可逆控制;(b)利用一对反作用酶相互转化ATP与ADP来控制核酸肽的自组装;(c)与Alexa ATP和核酸肽-NBD(50 mm)孵育的SA/DX5细胞的CLSM图像(比例尺: 5 mm)[39]
Fig. 6 (a) Interaction of assemblies of nucleopeptide with ATP or ADP and the reversible phase transition of the assemblies controlled by a pair of counteracting enzymes; (b) Employ a pair of counteracting enzymes to interconvert ATP and ADP for controlling the self-assembly of nucleopeptide; (c) CLSM images of the MES-SA/dx5 cells incubated with Alexa-ATP and NP1-NBD(50 mm). Scale bar: 5 mm[39]
图7 (a,b)单根纳米纤维与成束纳米纤维之间的转化;(c)激光共聚焦显微镜图像显示动态结构变化(从单个纤维到束状纤维再到单个纤维)对皮质星形胶质细胞性质的影响(从左到右),GFAP(绿色),细胞核(DAPI,蓝色),比例尺: 50 μm[18]
Fig. 7 (a, b) Conversion between individual nanofibers and nanofiber bundles; (c) Confocal microscopy images of astrocytes plated on individual fibers(left), on bundled fibers(center), and after switching from bundles to individual fibers(right). Staining for GFAP(green) and cell nuclei(DAPI, blue) reveals cells with na?ve morphology on substrates of individual fibers and reactive morphology on substrates of bundled fibers. Scale bar: 50 μm[18]
图8 (a)适配体和两亲性肽共组装物成纳米纤维;(b)根据自由适配体和apt-PAN系统的不同DNA浓度的过程曲线计算的初始降解速度的双倒数图[36]
Fig. 8 (a) Molecular representation of the coassembly of aptamer and diluent peptide amphiphiles; (b) Double-reciprocal plot of the initial degradation velocity calculated from the progress curves for various DNA concentrations for the free aptamer and apt-PAN systems[36]
图9 将多肽-DNA水凝胶3D打印成具有不同层次的结构:(a)一维点阵;(b)二维字母;(c)三维形状;(d)表明三维打印体具有一定的机械强度[40]
Fig. 9 3D printing of polypeptide-DNA hydrogel into 3D structures with blue dye added for visualization: (a) an array of printed droplets with an increasing gradient of layers. Inset, a lifted hydorgel with 20 layers; (b) the letters “THU” printed with 5 layers; (c) a triangle printed with 10 layers; (d) shows that the printed hydrogel structure is strong enough to be picked up with tweezers[40]
[1]
Wang J , Song J , Yang Z , He S , Yang Y , Feng X , Dou X , Shan A . J. Med. Chem., 2019,62(5):2286. https://www.ncbi.nlm.nih.gov/pubmed/30742437

doi: 10.1021/acs.jmedchem.8b01348     URL     pmid: 30742437
[2]
Lee H , Lim S I , Shin S H , Lim Y , Koh J W , Yang S . ACS Omega, 2019,4: 15694. https://www.ncbi.nlm.nih.gov/pubmed/31572872

URL     pmid: 31572872
[3]
Bai Q , Tang J , Wang H . Org. Lett., 2019,21(15):5858. https://www.ncbi.nlm.nih.gov/pubmed/31310555

URL     pmid: 31310555
[4]
Gui S , Huang Y , Zhu Y , Jin Y , Zhao R . ACS Appl. Mater. Interfaces, 2019,11(6):5804. https://www.ncbi.nlm.nih.gov/pubmed/30663882

doi: 10.1021/acsami.8b19076     URL     pmid: 30663882
[5]
Lv E , Ding J , Qin W . Anal. Chem., 2018,90(22):13600. https://www.ncbi.nlm.nih.gov/pubmed/30335975

doi: 10.1021/acs.analchem.8b03809     URL     pmid: 30335975
[6]
Singha N , Neogi S , Pramanik B , Das S , Dasgupta A , Ghosh R , Das D. ACS Appl. Polym. Mater., 2019,1: 2267. https://pubs.acs.org/doi/10.1021/acsapm.9b00594

doi: 10.1021/acsapm.9b00594     URL    
[7]
Strzalka J , Xu T , Tronin A , Wu S P , Miloradovic I , Kuzmenko I , Gog T , Therien M J , Blasiert J K. Nano Lett., 2006,6(11):2395. https://www.ncbi.nlm.nih.gov/pubmed/17090064

URL     pmid: 17090064
[8]
Cheng Z , Wu Y , Xiong Z , Gambhir S S , Chen X . Bioconjug. Chem., 2005,16(6):1433. https://www.ncbi.nlm.nih.gov/pubmed/16287239

URL     pmid: 16287239
[9]
Rouhbakhsh Z , Aili D , Martinsson E , Svärd A , Bäck M , Housaindokht M R , Nilsson K P R, Selegård R. ACS Omega, 2018,3(11):15066. https://www.ncbi.nlm.nih.gov/pubmed/31458172

URL     pmid: 31458172
[10]
Micklefield J. Curr. Med. Chem., 2012,8(10):1157. https://www.ncbi.nlm.nih.gov/pubmed/11472234

URL     pmid: 11472234
[11]
Shim T S , Estephan Z G , Qian Z , Prosser J H , Lee S Y , Chenoweth D M , Lee D , Park S J , Crocker J C. Nat. Nanotechnol., 2017,12(1):41. https://www.ncbi.nlm.nih.gov/pubmed/27775726

doi: 10.1038/nnano.2016.192     URL     pmid: 27775726
[12]
Zhang D Y , Seelig G. Nat. Chem.., 2011,3(2):103. https://www.ncbi.nlm.nih.gov/pubmed/21258382

URL     pmid: 21258382
[13]
Jones M R , Seeman N C , Mirkin C A . Science, 2015,347(6224):840.
[14]
Zhou C , Duan X , Liu N. Acc. Chem. Res., 2017,50(12):2906. https://www.ncbi.nlm.nih.gov/pubmed/28953361

doi: 10.1021/acs.accounts.7b00389     URL     pmid: 28953361
[15]
Liu N , Liedl T. . Chem. Rev., 2018,118(6):3032. https://www.ncbi.nlm.nih.gov/pubmed/29384370

URL     pmid: 29384370
[16]
Tan L H , Xing H , Lu Y. Acc. Chem. Res., 2014,47(6):1881. https://www.ncbi.nlm.nih.gov/pubmed/24871359

URL     pmid: 24871359
[17]
Hendrikse S I S , Gras S L , Ellis A V . ACS Nano, 2019,13(8):8512. https://www.ncbi.nlm.nih.gov/pubmed/31415144

doi: 10.1021/acsnano.9b06186     URL     pmid: 31415144
[18]
Freeman R , Han M , Álvarez Z , Lewis J A , Wester J R , Stephanopoulos N , McClendon M T, Lynsky C, Godbe J M, Sangji H, Luijten E, Stupp S I. Science, 2018,362(6416):808. https://www.ncbi.nlm.nih.gov/pubmed/30287619

URL     pmid: 30287619
[19]
Wijnands S P W , Meijer E W , Merkx M . Bioconjug. Chem., 2019,30(7):1905. https://www.ncbi.nlm.nih.gov/pubmed/30860819

doi: 10.1021/acs.bioconjchem.9b00095     URL     pmid: 30860819
[20]
Jia F , Li H , Chen R , Zhang K . Bioconjug. Chem., 2019,30(7):1880.
[21]
Chen Y , Liu Y. Chin. J. Org. Chem., 2012,32: 805. http://sioc-journal.cn/Jwk_yjhx/EN/10.6023/cjoc1201124

doi: 10.6023/cjoc1201124     URL    
[22]
Shahbazi M A , Bauleth-Ramos T , Santos H A. Adv. Ther., 2018,1(4):1800042.
[23]
Noteborn W E M , Zwagerman D N H , Talens V S , Maity C , van der Mee L , Poolman J M , Mytnyk S , van Esch J H , Kros A , Eelkema R , Kieltyka E R . Adv. Mater., 2017,29(12).
[24]
Kahn J S , Hu Y , Willner I . Accounts Chem. Res., 2017,50(4):680. https://pubs.acs.org/doi/10.1021/acs.accounts.6b00542

doi: 10.1021/acs.accounts.6b00542     URL    
[25]
Trinh T , Liao C , Toader V , Barlóg M , Bazzi H S , Li J , Sleiman H F. Nat. Chem., 2018,10(2):184. https://www.ncbi.nlm.nih.gov/pubmed/29359762

doi: 10.1038/nchem.2893     URL     pmid: 29359762
[26]
Rahbani J F , Vengut-Climent E , Chidchob P , Gidi Y , Trinh T , Cosa G , Sleiman H F. Adv. Healthc. Mater., 2018,7(6). https://www.ncbi.nlm.nih.gov/pubmed/29205903

doi: 10.1002/adhm.201701040     URL     pmid: 29205903
[27]
Chidchob P , Sleiman H F. Curr. Opin. Chem. Biol., 2018,46: 63. https://www.ncbi.nlm.nih.gov/pubmed/29751162

doi: 10.1016/j.cbpa.2018.04.012     URL     pmid: 29751162
[28]
Liu K , Zheng L , Ma C , Göstl R , Herrmann A. Chem. Soc. Rev., 2017,46(16):5147. https://www.ncbi.nlm.nih.gov/pubmed/28686247

doi: 10.1039/c7cs00165g     URL     pmid: 28686247
[29]
Ding X , Wang S. Prog. Pharm. Sci., 2015,39(2):105.
[30]
Ruff Y , Moyer T , Newcomb C J , Demeler B , Stupp S I . J. Am. Chem. Soc., 2013,135(16):6211. https://www.ncbi.nlm.nih.gov/pubmed/23574404

doi: 10.1021/ja4008003     URL     pmid: 23574404
[31]
Cao M , Wang Y , Zhao W , Qi R , Han Y , Wu R , Wang Y , Xu H . ACS Appl. Mater. Interfaces, 2018,10(29):24349. https://www.ncbi.nlm.nih.gov/pubmed/29979028

URL     pmid: 29979028
[32]
Ni R , Chau Y . J. Am. Chem. Soc., 2014,136(52):17902. https://www.ncbi.nlm.nih.gov/pubmed/25389763

URL     pmid: 25389763
[33]
Kadam V N , Saikrishnan K , Ganesh K N . J. Phys. Chem. C, 2018,122(25):14004.
[34]
Teengam P , Siangproh W , Tuantranont A , Vilaivan T , Chailapakul O , Henry C S. Anal. Chem., 2017,89(10):5428. https://www.ncbi.nlm.nih.gov/pubmed/28394582

doi: 10.1021/acs.analchem.7b00255     URL     pmid: 28394582
[35]
Chen P , Li C , Liu D , Li Z . Macromolecules, 2012,45(24):9579. https://pubs.acs.org/doi/10.1021/ma302233m

doi: 10.1021/ma302233m     URL    
[36]
Serrano C M , Freeman R , Godbe J , Lewis J A , Stupp S I. ACS Appl. Bio Mater., 2019,2(7):2955. https://pubs.acs.org/doi/10.1021/acsabm.9b00310

doi: 10.1021/acsabm.9b00310     URL    
[37]
Li C , Chen P , Shao Y , Zhou X , Wu Y , Yang Z , Li Z , Weil T , Liu D . Small, 2015,11(9/10):1138. http://doi.wiley.com/10.1002/smll.v11.9-10

doi: 10.1002/smll.v11.9-10     URL    
[38]
Daly M L , Gao Y , Freeman R . Bioconjug. Chem. 2019,30: 1864. https://www.ncbi.nlm.nih.gov/pubmed/31181892

doi: 10.1021/acs.bioconjchem.9b00271     URL     pmid: 31181892
[39]
Wang H , Feng Z , Qin Y , Wang J , Xu B . Angew. Chemie-Int. Ed., 2018,57(18):4931. http://doi.wiley.com/10.1002/anie.201712834

doi: 10.1002/anie.201712834     URL    
[40]
Li C , Faulkner-Jones A , Dun A R , Jin J, Chen P, Xing Y, Yang Z, Li Z, Shu W, Liu D, Duncan R R. Angew. Chemie-Int. Ed., 2015,54(13):3957.
[41]
Breger J C , Buckhout-White S , Walper S A , Oh E , Susumu K , Ancona M G , Medintz I L. Nano Futur., 2017,1(1):011002.
[42]
Lim K S , Lee D Y , Valencia G M , Bull D A , Won Y W. ACS Macro Lett., 2017,6(2):98.
[43]
Stephanopoulos N . Bioconjug. Chem., 2019,30: 1915. https://www.ncbi.nlm.nih.gov/pubmed/31082220

doi: 10.1021/acs.bioconjchem.9b00259     URL     pmid: 31082220
[44]
George M , Grzybowski B . Science, 2002,295(5564):2418. https://www.ncbi.nlm.nih.gov/pubmed/11923529

doi: 10.1126/science.1070821     URL     pmid: 11923529
[45]
Gorin G . J. Chem. Educ., 1964,41(1):44. https://pubs.acs.org/doi/abs/10.1021/ed041p44

doi: 10.1021/ed041p44     URL    
[46]
Fukada K , Maeda H . J. Phys. Chem., 1990,94(9):3843. https://pubs.acs.org/doi/abs/10.1021/j100372a090

doi: 10.1021/j100372a090     URL    
[47]
Jeffrey D , Hartgerink E B S I S. Am. Assoc. Adv. Sci., 2001,294(5547):1684.
[48]
Jeffrey D. Hartgerink E B, Stupp S I. Proc. Natl. Acad. Sci. U S A., 2002,99(8):5133. https://www.ncbi.nlm.nih.gov/pubmed/11929981

doi: 10.1073/pnas.072699999     URL     pmid: 11929981
[49]
Kunitake T. Angew. Chemie Int. Ed., 1992,31(6):709. http://doi.wiley.com/10.1002/%28ISSN%291521-3773

doi: 10.1002/(ISSN)1521-3773     URL    
[50]
Cui H , Webber M J , Stupp S I . Biopolymers, 2010,94(1):1. https://www.ncbi.nlm.nih.gov/pubmed/20091874

doi: 10.1002/bip.21328     URL     pmid: 20091874
[51]
Simmel F C , Yurke B , Singh H R. Chem. Rev., 2019,119: 6326. https://www.ncbi.nlm.nih.gov/pubmed/30714375

URL     pmid: 30714375
[52]
Shao Y , Jia H , Cao T , Liu D. Acc. Chem. Res., 2017,50(4):659. https://www.ncbi.nlm.nih.gov/pubmed/28299927

URL     pmid: 28299927
[53]
Zhang D Y , Seeling G. Nat. Chem.., 2011,3: 103. https://www.ncbi.nlm.nih.gov/pubmed/21258382

doi: 10.1038/nchem.957     URL     pmid: 21258382
[54]
许小丁 ( Xu X D ), 陈昌盛(Chen C S), 陈荆晓(Chen J X), 张先正(Zhang X Z), 卓仁禧(Zhuo R X). 中国科学: 化学(Scientia Sinica Chimica), 2011,41(2):221.
[55]
Datta B , Schuster G B , McCook A, Harvey S C, Zakrzewska K. J. Am. Chem. Soc., 2006,128(45):14428. https://www.ncbi.nlm.nih.gov/pubmed/17090004

URL     pmid: 17090004
[56]
Datta B , Schuster G B . J. Am. Chem. Soc., 2008,130(10):2965. https://www.ncbi.nlm.nih.gov/pubmed/18266359

doi: 10.1021/ja0726106     URL     pmid: 18266359
[57]
Carneiro K M M , Avakyan N , Sleiman H F . Wires. Nanomed. Nanobio., 2013,5(3):266. http://doi.wiley.com/10.1002/wnan.1218

doi: 10.1002/wnan.1218     URL    
[58]
Zhou K , Ke Y , Wang Q . J. Am. Chem. Soc., 2018,140(26):8074. https://www.ncbi.nlm.nih.gov/pubmed/29932333

doi: 10.1021/jacs.8b03914     URL     pmid: 29932333
[59]
Ijäs H , Hakaste I , Shen B , Kostiainen M A , Linko V . ACS Nano, 2019,13(5):5959. https://www.ncbi.nlm.nih.gov/pubmed/30990664

doi: 10.1021/acsnano.9b01857     URL     pmid: 30990664
[60]
Mukhortava A , Schlierf M . Bioconjug. Chem., 2016,27(7):1559. https://www.ncbi.nlm.nih.gov/pubmed/27322198

doi: 10.1021/acs.bioconjchem.6b00120     URL     pmid: 27322198
[61]
Cremers G A O , Rosier B J H M, Riera Brillas R , Albertazzi L , de Greef T F A. Bioconjug. Chem., 2019,30: 2384. https://www.ncbi.nlm.nih.gov/pubmed/31438665

doi: 10.1021/acs.bioconjchem.9b00490     URL     pmid: 31438665
[62]
Trads J B , Tørring T , Gothelf K V. Acc. Chem. Res., 2017,50(6):1367. https://www.ncbi.nlm.nih.gov/pubmed/28485577

URL     pmid: 28485577
[63]
Hernandez-Garcia A , Estrich N A , Werten M W T, Van der Maarel J R C, LaBean T H, De Wolf F A, Cohen Stuart M A, De Vries R. ACS Nano, 2017,11(1):144. https://www.ncbi.nlm.nih.gov/pubmed/27936577

doi: 10.1021/acsnano.6b05938     URL     pmid: 27936577
[64]
Lee H Y , Ren C , Park S Y .J. Phys. Chem. C, 2016,120(32):18307.
[65]
Green L S , Jellinek D , Jenison R , Östman A , Heldin C H , Janjic N . Biochemistry, 1996,35(45):14413. https://www.ncbi.nlm.nih.gov/pubmed/8916928

doi: 10.1021/bi961544+     URL     pmid: 8916928
[1] 李良春, 郑仁林, 黄毅, 孙荣琴. 多组分自组装小分子水凝胶中的自分类组装[J]. 化学进展, 2023, 35(2): 274-286.
[2] 王萌, 宋贺, 李烨文. 三维自组装蓝相液晶光子晶体[J]. 化学进展, 2022, 34(8): 1734-1747.
[3] 韩冬雪, 金雪, 苗碗根, 焦体峰, 段鹏飞. 超分子组装体激发态手性的响应性[J]. 化学进展, 2022, 34(6): 1252-1262.
[4] 尹航, 李智, 郭晓峰, 冯岸超, 张立群, 汤华燊. RAFT链转移剂的选用原则及通用型RAFT链转移剂[J]. 化学进展, 2022, 34(6): 1298-1307.
[5] 刘玉玲, 胡腾达, 李伊莲, 林洋, Borsali Redouane, 廖英杰. 嵌段共聚物薄膜快速自组装方法[J]. 化学进展, 2022, 34(3): 609-615.
[6] 李红, 史晓丹, 李洁龄. 肽自组装水凝胶的制备及在生物医学中的应用[J]. 化学进展, 2022, 34(3): 568-579.
[7] 王玉冰, 陈杰, 延卫, 崔建文. 共轭微孔聚合物的制备与应用[J]. 化学进展, 2021, 33(5): 838-854.
[8] 闫楚璇, 李青璘, 巩正奇, 陈颖芝, 王鲁宁. 纳米有机半导体光催化剂[J]. 化学进展, 2021, 33(11): 1917-1934.
[9] 冯业娜, 刘书河, 张书博, 薛彤, 庄鸿麟, 冯岸超. 基于聚合诱导自组装制备二氧化硅/聚合物纳米复合材料[J]. 化学进展, 2021, 33(11): 1953-1963.
[10] 肖晶晶, 王牧, 张伟杰, 赵秀英, 冯岸超, 张立群. 铅卤钙钛矿-聚合物复合材料的制备及应用[J]. 化学进展, 2021, 33(10): 1731-1740.
[11] 卢芸, 李景鹏, 张燕, 仲国瑞, 刘波, 王慧庆. 木基炭微纳功能骨架[J]. 化学进展, 2020, 32(7): 906-916.
[12] 智康康, 杨鑫. 天然产物凝胶及其凝胶质[J]. 化学进展, 2019, 31(9): 1314-1328.
[13] 林代武, 邢起国, 王跃飞, 齐崴, 苏荣欣, 何志敏. 多肽超分子手性自组装与应用[J]. 化学进展, 2019, 31(12): 1623-1636.
[14] 刘耀华, 刘育. 基于偶氮功能基的光控超分子组装[J]. 化学进展, 2019, 31(11): 1528-1539.
[15] 徐子悦, 张运昌, 林佳乐, 王辉, 张丹维, 黎占亭. 药物输送体系构筑中的超分子组装策略[J]. 化学进展, 2019, 31(11): 1540-1549.