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化学进展 2014, Vol. 26 Issue (08): 1378-1394 DOI: 10.7536/PC140312 前一篇   后一篇

• 综述与评论 •

生物法与化学法制备硫酸软骨素

石玉刚*1,2, 党亚丽3, 刘玉华1, 白雪1   

  1. 1. 浙江工商大学食品与生物工程学院 浙江省食品安全重点实验室 杭州 310035;
    2. 多伦多大学化学系 多伦多M5S;
    3. 浙江省医学科学院保健食品研究所 杭州 310013
  • 收稿日期:2014-03-01 修回日期:2014-04-01 出版日期:2014-08-15 发布日期:2014-06-10
  • 通讯作者: 石玉刚 E-mail:yugangshi@mail.zjgsu.edu.cn
  • 基金资助:

    国家自然科学基金项目（No. 21106131，31101344）、浙江省分析测试项目（No. 2013C37043）、浙江省教育厅科研项目（No. Y201016439）和浙江工商大学人才引进科研基金（No.09-57）资助

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Microbial and Chemical Production of Chondroitin Sulfate

Shi Yugang*1,2, Dang Yali3, Liu Yuhua1, Bai Xue1   

  1. 1. Food Safety Key Lab of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China;
    2. Department of Chemistry, University of Toronto, Toronto M5S;
    3. Institute of Health Food, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China
  • Received:2014-03-01 Revised:2014-04-01 Online:2014-08-15 Published:2014-06-10
  • Supported by:

    The work was supported by the National Natural Science Foundation of China (No. 21106131, 31101344), the Analysis and Measurement Foundation of Zhejiang Province (No. 2013C37043), the Research Foundation of Educational Commission of Zhejiang Province of China (No. Y201016439) and the Scientific Research Fund for the New Talents of Zhejiang Gongshang University (No. 09-57)

硫酸软骨素（ChS）是一种天然的酸性黏多糖，生物活性多样，在医学、生物、制药、食品、化妆品和材料等领域均有广泛应用，特别是在治愈骨关节病上具有颇为理想的效果。本文系统地总结了有关硫酸软骨素及其类似物的生物与化学制备方法的研究进展，如高产微生物菌株筛选与改良，培养条件优化与调控，汇聚式合成策略构建，经济易得的起始物原料等，并指出制备硫酸软骨素中存在的问题以及发展方向。

关键词： 硫酸软骨素, 化学合成, 生物催化, 硫酸化代码

Chondroitin sulfates (ChS) are a complex polysaccharide having important structural and protective functions and play important roles in many biological processes. It has been widely applied in a variety of fields, such as medicine, biotechnology, pharmacy, food, cosmetics and textiles. This mini-review focuses on the recent advances in microbial and chemical synthesis of such molecules. The productive strains, fermentation conditions, convergent strategies, use of new starting materials, and syntheses of all the ChS variant oligosaccharides are discussed. In addition, suggestions for further studies in syntheses of ChS are proposed based on current research.

Contents
1 Introduction
2 Biotechnological production of ChS and ChS-like products
2.1 Enzymatic synthesis of ChS and ChS-like products
2.2 Microbial production of ChS and ChS-like products
3 Chemical synthesis of ChS and ChS-like products
4 Conclusion and outlook

Key words: chondroitin sulfate, chemical synthesis, enzymatic synthesis, sulfo code

中图分类号: 

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生物法与化学法制备硫酸软骨素